Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1265 and GFF1266 are separated by 66 nucleotides GFF1266 and GFF1267 are separated by 4 nucleotides
GFF1265 - cAMP-binding protein, at 580,449 to 581,381
GFF1265
GFF1266 - Cysteine desulfurase (EC 2.8.1.7), at 581,448 to 583,253
GFF1266
GFF1267 - Probable lipoprotein signal peptide, at 583,258 to 584,262
GFF1267
Position (kb) 581
582
583
584 Strain fitness (log2 ratio) -2
-1
0
1
2 at 580.599 kb on + strand, within GFF1265 at 580.600 kb on - strand, within GFF1265 at 580.770 kb on + strand, within GFF1265 at 581.271 kb on + strand, within GFF1265 at 581.272 kb on - strand, within GFF1265 at 581.287 kb on - strand, within GFF1265 at 581.352 kb on + strand at 581.353 kb on - strand at 581.353 kb on - strand at 581.373 kb on + strand at 581.373 kb on + strand at 581.374 kb on - strand at 581.374 kb on - strand at 581.379 kb on + strand at 581.380 kb on - strand at 581.380 kb on - strand at 581.612 kb on + strand at 581.613 kb on - strand at 581.925 kb on + strand, within GFF1266 at 581.925 kb on + strand, within GFF1266 at 582.820 kb on + strand, within GFF1266 at 582.820 kb on + strand, within GFF1266 at 582.821 kb on - strand, within GFF1266 at 582.821 kb on - strand, within GFF1266 at 582.900 kb on + strand, within GFF1266 at 582.901 kb on - strand, within GFF1266 at 582.901 kb on - strand, within GFF1266 at 582.901 kb on - strand, within GFF1266 at 582.957 kb on + strand, within GFF1266 at 582.958 kb on - strand, within GFF1266 at 582.958 kb on - strand, within GFF1266 at 583.051 kb on - strand, within GFF1266 at 583.179 kb on + strand at 583.179 kb on + strand at 583.180 kb on - strand at 583.782 kb on - strand, within GFF1267 at 583.997 kb on + strand, within GFF1267
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 580,599 + GFF1265 0.16 +0.1 580,600 - GFF1265 0.16 +0.2 580,770 + GFF1265 0.34 +0.0 581,271 + GFF1265 0.88 -0.0 581,272 - GFF1265 0.88 -0.9 581,287 - GFF1265 0.90 +0.1 581,352 + -0.2 581,353 - -0.2 581,353 - -0.4 581,373 + +0.7 581,373 + -0.4 581,374 - -0.2 581,374 - +1.0 581,379 + +1.0 581,380 - +0.9 581,380 - -0.3 581,612 + -0.1 581,613 - +0.5 581,925 + GFF1266 0.26 +0.1 581,925 + GFF1266 0.26 +0.1 582,820 + GFF1266 0.76 -0.3 582,820 + GFF1266 0.76 -1.3 582,821 - GFF1266 0.76 +0.2 582,821 - GFF1266 0.76 +0.4 582,900 + GFF1266 0.80 -1.2 582,901 - GFF1266 0.80 +0.2 582,901 - GFF1266 0.80 -1.8 582,901 - GFF1266 0.80 -0.1 582,957 + GFF1266 0.84 +0.4 582,958 - GFF1266 0.84 +0.2 582,958 - GFF1266 0.84 -1.0 583,051 - GFF1266 0.89 -0.3 583,179 + +0.2 583,179 + -1.1 583,180 - +1.9 583,782 - GFF1267 0.52 +0.0 583,997 + GFF1267 0.74 -0.5
Or see this region's nucleotide sequence