Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1110 and GFF1111 are separated by 27 nucleotides GFF1111 and GFF1112 are separated by 137 nucleotides GFF1112 and GFF1113 are separated by 55 nucleotides GFF1113 and GFF1114 overlap by 1 nucleotides
GFF1110 - Recombination protein RecR, at 427,822 to 428,412
GFF1110
GFF1111 - probable membrane protein NMA1176, at 428,440 to 428,832
GFF1111
GFF1112 - Hydroxymethylpyrimidine ABC transporter, substrate-binding component, at 428,970 to 430,007
GFF1112
GFF1113 - ABC transporter, ATP-binding protein (cluster 1, maltose/g3p/polyamine/iron); ABC transporter, ATP-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 430,063 to 430,878
GFF1113
GFF1114 - ABC transporter, permease protein (cluster 10, nitrate/sulfonate/bicarbonate), at 430,878 to 431,720
GFF1114
Position (kb) 428
429
430
431 Strain fitness (log2 ratio) -2
-1
0
1
2 at 428.131 kb on + strand, within GFF1110 at 428.131 kb on + strand, within GFF1110 at 428.132 kb on - strand, within GFF1110 at 428.132 kb on - strand, within GFF1110 at 428.132 kb on - strand, within GFF1110 at 428.132 kb on - strand, within GFF1110 at 428.132 kb on - strand, within GFF1110 at 428.132 kb on - strand, within GFF1110 at 428.132 kb on - strand, within GFF1110 at 428.365 kb on + strand at 428.365 kb on + strand at 428.365 kb on + strand at 428.366 kb on - strand at 428.366 kb on - strand at 428.467 kb on + strand at 428.467 kb on + strand at 428.467 kb on + strand at 428.467 kb on + strand at 428.468 kb on - strand at 428.468 kb on - strand at 428.468 kb on - strand at 428.468 kb on - strand at 428.468 kb on - strand at 428.468 kb on - strand at 428.468 kb on - strand at 428.468 kb on - strand at 428.468 kb on - strand at 428.501 kb on - strand, within GFF1111 at 428.713 kb on + strand, within GFF1111 at 428.713 kb on + strand, within GFF1111 at 428.713 kb on + strand, within GFF1111 at 428.713 kb on + strand, within GFF1111 at 428.713 kb on + strand, within GFF1111 at 428.713 kb on + strand, within GFF1111 at 428.714 kb on - strand, within GFF1111 at 428.714 kb on - strand, within GFF1111 at 428.714 kb on - strand, within GFF1111 at 428.714 kb on - strand, within GFF1111 at 428.714 kb on - strand, within GFF1111 at 428.734 kb on + strand, within GFF1111 at 428.734 kb on + strand, within GFF1111 at 428.734 kb on + strand, within GFF1111 at 428.734 kb on + strand, within GFF1111 at 428.734 kb on + strand, within GFF1111 at 428.734 kb on + strand, within GFF1111 at 428.735 kb on - strand, within GFF1111 at 428.735 kb on - strand, within GFF1111 at 428.735 kb on - strand, within GFF1111 at 428.735 kb on - strand, within GFF1111 at 428.735 kb on - strand, within GFF1111 at 428.746 kb on + strand, within GFF1111 at 428.746 kb on + strand, within GFF1111 at 428.747 kb on - strand, within GFF1111 at 428.747 kb on - strand, within GFF1111 at 428.747 kb on - strand, within GFF1111 at 428.747 kb on - strand, within GFF1111 at 428.747 kb on - strand, within GFF1111 at 428.830 kb on + strand at 428.830 kb on + strand at 428.869 kb on + strand at 428.869 kb on + strand at 428.869 kb on + strand at 428.869 kb on + strand at 428.869 kb on + strand at 428.869 kb on + strand at 428.870 kb on - strand at 428.870 kb on - strand at 428.921 kb on + strand at 428.921 kb on + strand at 428.922 kb on - strand at 429.105 kb on + strand, within GFF1112 at 429.105 kb on + strand, within GFF1112 at 429.105 kb on + strand, within GFF1112 at 429.105 kb on + strand, within GFF1112 at 429.105 kb on + strand, within GFF1112 at 429.106 kb on - strand, within GFF1112 at 429.106 kb on - strand, within GFF1112 at 429.108 kb on + strand, within GFF1112 at 429.108 kb on + strand, within GFF1112 at 429.108 kb on + strand, within GFF1112 at 429.108 kb on + strand, within GFF1112 at 429.108 kb on + strand, within GFF1112 at 429.109 kb on - strand, within GFF1112 at 429.109 kb on - strand, within GFF1112 at 429.141 kb on + strand, within GFF1112 at 429.141 kb on + strand, within GFF1112 at 429.142 kb on - strand, within GFF1112 at 429.142 kb on - strand, within GFF1112 at 429.145 kb on + strand, within GFF1112 at 429.247 kb on - strand, within GFF1112 at 429.247 kb on - strand, within GFF1112 at 429.247 kb on - strand, within GFF1112 at 429.357 kb on + strand, within GFF1112 at 429.357 kb on + strand, within GFF1112 at 429.358 kb on - strand, within GFF1112 at 429.358 kb on - strand, within GFF1112 at 429.359 kb on + strand, within GFF1112 at 429.359 kb on + strand, within GFF1112 at 429.360 kb on - strand, within GFF1112 at 429.477 kb on + strand, within GFF1112 at 429.477 kb on + strand, within GFF1112 at 429.477 kb on + strand, within GFF1112 at 429.477 kb on + strand, within GFF1112 at 429.477 kb on + strand, within GFF1112 at 429.477 kb on + strand, within GFF1112 at 429.477 kb on + strand, within GFF1112 at 429.478 kb on - strand, within GFF1112 at 429.478 kb on - strand, within GFF1112 at 429.478 kb on - strand, within GFF1112 at 429.478 kb on - strand, within GFF1112 at 429.478 kb on - strand, within GFF1112 at 429.478 kb on - strand, within GFF1112 at 429.478 kb on - strand, within GFF1112 at 429.666 kb on + strand, within GFF1112 at 429.666 kb on + strand, within GFF1112 at 429.667 kb on - strand, within GFF1112 at 429.667 kb on - strand, within GFF1112 at 429.667 kb on - strand, within GFF1112 at 429.795 kb on + strand, within GFF1112 at 429.795 kb on + strand, within GFF1112 at 429.795 kb on + strand, within GFF1112 at 429.795 kb on + strand, within GFF1112 at 429.796 kb on - strand, within GFF1112 at 429.796 kb on - strand, within GFF1112 at 429.796 kb on - strand, within GFF1112 at 429.796 kb on - strand, within GFF1112 at 429.796 kb on - strand, within GFF1112 at 429.796 kb on - strand, within GFF1112 at 429.796 kb on - strand, within GFF1112 at 429.796 kb on - strand, within GFF1112 at 429.819 kb on + strand, within GFF1112 at 429.819 kb on + strand, within GFF1112 at 429.820 kb on - strand, within GFF1112 at 429.960 kb on + strand at 429.960 kb on + strand at 429.960 kb on + strand at 429.960 kb on + strand at 429.960 kb on + strand at 429.961 kb on - strand at 429.961 kb on - strand at 430.135 kb on + strand at 430.135 kb on + strand at 430.135 kb on + strand at 430.315 kb on + strand, within GFF1113 at 430.316 kb on - strand, within GFF1113 at 430.316 kb on - strand, within GFF1113 at 430.316 kb on - strand, within GFF1113 at 430.316 kb on - strand, within GFF1113 at 430.849 kb on + strand at 430.849 kb on + strand at 430.850 kb on - strand at 430.850 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 428,131 + GFF1110 0.52 -0.4 428,131 + GFF1110 0.52 +1.1 428,132 - GFF1110 0.52 -0.8 428,132 - GFF1110 0.52 +0.6 428,132 - GFF1110 0.52 -0.6 428,132 - GFF1110 0.52 -0.1 428,132 - GFF1110 0.52 +0.0 428,132 - GFF1110 0.52 -0.2 428,132 - GFF1110 0.52 +0.0 428,365 + +1.9 428,365 + -1.0 428,365 + +0.3 428,366 - -1.7 428,366 - -0.7 428,467 + +1.6 428,467 + -1.4 428,467 + +0.1 428,467 + +0.7 428,468 - -0.3 428,468 - -0.4 428,468 - -0.4 428,468 - -0.0 428,468 - -1.4 428,468 - -1.6 428,468 - -1.3 428,468 - -0.3 428,468 - -0.3 428,501 - GFF1111 0.16 +1.6 428,713 + GFF1111 0.69 +0.1 428,713 + GFF1111 0.69 +0.5 428,713 + GFF1111 0.69 -0.3 428,713 + GFF1111 0.69 +0.8 428,713 + GFF1111 0.69 +0.6 428,713 + GFF1111 0.69 -0.6 428,714 - GFF1111 0.70 -0.0 428,714 - GFF1111 0.70 +1.1 428,714 - GFF1111 0.70 -0.4 428,714 - GFF1111 0.70 +0.8 428,714 - GFF1111 0.70 -0.8 428,734 + GFF1111 0.75 +1.0 428,734 + GFF1111 0.75 -0.6 428,734 + GFF1111 0.75 -0.9 428,734 + GFF1111 0.75 +0.7 428,734 + GFF1111 0.75 -0.1 428,734 + GFF1111 0.75 -0.4 428,735 - GFF1111 0.75 +0.5 428,735 - GFF1111 0.75 -0.8 428,735 - GFF1111 0.75 -1.5 428,735 - GFF1111 0.75 -0.1 428,735 - GFF1111 0.75 -0.9 428,746 + GFF1111 0.78 +0.2 428,746 + GFF1111 0.78 -0.0 428,747 - GFF1111 0.78 +0.6 428,747 - GFF1111 0.78 +0.3 428,747 - GFF1111 0.78 +0.8 428,747 - GFF1111 0.78 +0.7 428,747 - GFF1111 0.78 +0.2 428,830 + -0.3 428,830 + -0.7 428,869 + +1.0 428,869 + -0.3 428,869 + +0.4 428,869 + -1.4 428,869 + -1.3 428,869 + +0.3 428,870 - +0.7 428,870 - -1.3 428,921 + -0.0 428,921 + +0.1 428,922 - -0.3 429,105 + GFF1112 0.13 -0.0 429,105 + GFF1112 0.13 +0.7 429,105 + GFF1112 0.13 -0.4 429,105 + GFF1112 0.13 +1.1 429,105 + GFF1112 0.13 +0.6 429,106 - GFF1112 0.13 -0.3 429,106 - GFF1112 0.13 -1.6 429,108 + GFF1112 0.13 +0.7 429,108 + GFF1112 0.13 +0.1 429,108 + GFF1112 0.13 -1.2 429,108 + GFF1112 0.13 -0.4 429,108 + GFF1112 0.13 +1.3 429,109 - GFF1112 0.13 -0.1 429,109 - GFF1112 0.13 +1.1 429,141 + GFF1112 0.16 +0.9 429,141 + GFF1112 0.16 -0.6 429,142 - GFF1112 0.17 +1.6 429,142 - GFF1112 0.17 -1.0 429,145 + GFF1112 0.17 -0.4 429,247 - GFF1112 0.27 +0.6 429,247 - GFF1112 0.27 -0.0 429,247 - GFF1112 0.27 +1.0 429,357 + GFF1112 0.37 +0.0 429,357 + GFF1112 0.37 -0.5 429,358 - GFF1112 0.37 +0.1 429,358 - GFF1112 0.37 -0.4 429,359 + GFF1112 0.37 -0.1 429,359 + GFF1112 0.37 -0.8 429,360 - GFF1112 0.38 -2.4 429,477 + GFF1112 0.49 -1.0 429,477 + GFF1112 0.49 -0.2 429,477 + GFF1112 0.49 -0.2 429,477 + GFF1112 0.49 -0.7 429,477 + GFF1112 0.49 -0.3 429,477 + GFF1112 0.49 +1.3 429,477 + GFF1112 0.49 +0.8 429,478 - GFF1112 0.49 -1.1 429,478 - GFF1112 0.49 -0.6 429,478 - GFF1112 0.49 -0.1 429,478 - GFF1112 0.49 +1.6 429,478 - GFF1112 0.49 -0.0 429,478 - GFF1112 0.49 -0.7 429,478 - GFF1112 0.49 -0.4 429,666 + GFF1112 0.67 -0.2 429,666 + GFF1112 0.67 +0.8 429,667 - GFF1112 0.67 -0.5 429,667 - GFF1112 0.67 +0.1 429,667 - GFF1112 0.67 -1.3 429,795 + GFF1112 0.79 +0.3 429,795 + GFF1112 0.79 -2.0 429,795 + GFF1112 0.79 -1.6 429,795 + GFF1112 0.79 -1.0 429,796 - GFF1112 0.80 +0.8 429,796 - GFF1112 0.80 -0.4 429,796 - GFF1112 0.80 -0.9 429,796 - GFF1112 0.80 -1.3 429,796 - GFF1112 0.80 -0.0 429,796 - GFF1112 0.80 +0.1 429,796 - GFF1112 0.80 -0.3 429,796 - GFF1112 0.80 +0.9 429,819 + GFF1112 0.82 +1.4 429,819 + GFF1112 0.82 +1.0 429,820 - GFF1112 0.82 -0.1 429,960 + -0.4 429,960 + -0.2 429,960 + +0.7 429,960 + +0.5 429,960 + +0.8 429,961 - +1.9 429,961 - -0.2 430,135 + -0.4 430,135 + -0.3 430,135 + +2.0 430,315 + GFF1113 0.31 -0.2 430,316 - GFF1113 0.31 +0.2 430,316 - GFF1113 0.31 -1.0 430,316 - GFF1113 0.31 -0.4 430,316 - GFF1113 0.31 -0.3 430,849 + -1.6 430,849 + -0.1 430,850 - -0.0 430,850 - -1.1
Or see this region's nucleotide sequence