Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF85 and GFF86 are separated by 41 nucleotides GFF86 and GFF87 overlap by 4 nucleotides GFF87 and GFF88 are separated by 7 nucleotides
GFF85 - Transcriptional regulator, LysR family, at 101,576 to 102,475
GFF85
GFF86 - Cell morphology protein, at 102,517 to 103,191
GFF86
GFF87 - no description, at 103,188 to 103,868
GFF87
GFF88 - Probable poly(beta-D-mannuronate) O-acetylase (EC 2.3.1.-), at 103,876 to 105,270
GFF88
Position (kb)
103
104 Strain fitness (log2 ratio)
-1
0
1
2 at 102.444 kb on + strand at 102.444 kb on + strand at 102.482 kb on + strand at 102.483 kb on - strand at 102.483 kb on - strand at 102.589 kb on + strand, within GFF86 at 102.590 kb on - strand, within GFF86 at 102.590 kb on - strand, within GFF86 at 102.590 kb on - strand, within GFF86 at 102.590 kb on - strand, within GFF86 at 102.590 kb on - strand, within GFF86 at 102.670 kb on + strand, within GFF86 at 102.968 kb on - strand, within GFF86 at 102.980 kb on - strand, within GFF86 at 102.980 kb on - strand, within GFF86 at 103.273 kb on - strand, within GFF87 at 103.273 kb on - strand, within GFF87 at 103.405 kb on - strand, within GFF87 at 103.602 kb on + strand, within GFF87 at 103.602 kb on + strand, within GFF87 at 103.603 kb on - strand, within GFF87 at 103.603 kb on - strand, within GFF87 at 103.603 kb on - strand, within GFF87 at 103.934 kb on - strand at 103.934 kb on - strand at 103.934 kb on - strand at 103.993 kb on + strand at 103.993 kb on + strand at 103.994 kb on - strand at 103.994 kb on - strand at 103.994 kb on - strand at 103.994 kb on - strand at 103.994 kb on - strand at 104.138 kb on + strand, within GFF88 at 104.139 kb on - strand, within GFF88 at 104.139 kb on - strand, within GFF88 at 104.139 kb on - strand, within GFF88 at 104.139 kb on - strand, within GFF88 at 104.139 kb on - strand, within GFF88 at 104.139 kb on - strand, within GFF88 at 104.139 kb on - strand, within GFF88 at 104.150 kb on - strand, within GFF88 at 104.272 kb on + strand, within GFF88 at 104.272 kb on + strand, within GFF88 at 104.272 kb on + strand, within GFF88 at 104.273 kb on - strand, within GFF88 at 104.273 kb on - strand, within GFF88 at 104.335 kb on + strand, within GFF88 at 104.335 kb on + strand, within GFF88 at 104.335 kb on + strand, within GFF88 at 104.335 kb on + strand, within GFF88 at 104.336 kb on - strand, within GFF88 at 104.336 kb on - strand, within GFF88 at 104.336 kb on - strand, within GFF88 at 104.336 kb on - strand, within GFF88 at 104.569 kb on + strand, within GFF88 at 104.570 kb on - strand, within GFF88 at 104.570 kb on - strand, within GFF88 at 104.604 kb on + strand, within GFF88 at 104.604 kb on + strand, within GFF88 at 104.712 kb on + strand, within GFF88 at 104.712 kb on + strand, within GFF88 at 104.712 kb on + strand, within GFF88 at 104.713 kb on - strand, within GFF88 at 104.713 kb on - strand, within GFF88 at 104.713 kb on - strand, within GFF88 at 104.713 kb on - strand, within GFF88 at 104.740 kb on + strand, within GFF88 at 104.741 kb on - strand, within GFF88 at 104.741 kb on - strand, within GFF88 at 104.746 kb on + strand, within GFF88 at 104.857 kb on + strand, within GFF88
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 102,444 + -1.0 102,444 + +0.6 102,482 + -0.3 102,483 - +0.2 102,483 - -0.3 102,589 + GFF86 0.11 +0.1 102,590 - GFF86 0.11 -0.7 102,590 - GFF86 0.11 +0.9 102,590 - GFF86 0.11 -0.9 102,590 - GFF86 0.11 +0.0 102,590 - GFF86 0.11 -0.4 102,670 + GFF86 0.23 -0.2 102,968 - GFF86 0.67 +0.5 102,980 - GFF86 0.69 -0.6 102,980 - GFF86 0.69 +2.0 103,273 - GFF87 0.12 -0.6 103,273 - GFF87 0.12 +1.1 103,405 - GFF87 0.32 -0.4 103,602 + GFF87 0.61 -0.7 103,602 + GFF87 0.61 -0.9 103,603 - GFF87 0.61 +0.3 103,603 - GFF87 0.61 -1.2 103,603 - GFF87 0.61 +0.3 103,934 - +0.2 103,934 - -0.7 103,934 - -0.7 103,993 + -1.1 103,993 + -1.3 103,994 - +0.0 103,994 - +1.1 103,994 - -0.7 103,994 - -0.4 103,994 - -0.0 104,138 + GFF88 0.19 -1.6 104,139 - GFF88 0.19 +0.0 104,139 - GFF88 0.19 +0.4 104,139 - GFF88 0.19 -0.0 104,139 - GFF88 0.19 -0.9 104,139 - GFF88 0.19 -0.1 104,139 - GFF88 0.19 -0.1 104,139 - GFF88 0.19 -0.3 104,150 - GFF88 0.20 -1.0 104,272 + GFF88 0.28 -0.5 104,272 + GFF88 0.28 -0.5 104,272 + GFF88 0.28 -1.7 104,273 - GFF88 0.28 +0.2 104,273 - GFF88 0.28 -0.5 104,335 + GFF88 0.33 -0.7 104,335 + GFF88 0.33 -0.3 104,335 + GFF88 0.33 -1.1 104,335 + GFF88 0.33 -1.3 104,336 - GFF88 0.33 -0.9 104,336 - GFF88 0.33 -0.2 104,336 - GFF88 0.33 -1.0 104,336 - GFF88 0.33 +0.2 104,569 + GFF88 0.50 -1.7 104,570 - GFF88 0.50 +0.3 104,570 - GFF88 0.50 +0.3 104,604 + GFF88 0.52 -0.0 104,604 + GFF88 0.52 -0.0 104,712 + GFF88 0.60 -1.1 104,712 + GFF88 0.60 -0.3 104,712 + GFF88 0.60 -0.5 104,713 - GFF88 0.60 +0.1 104,713 - GFF88 0.60 -0.3 104,713 - GFF88 0.60 +0.7 104,713 - GFF88 0.60 +0.1 104,740 + GFF88 0.62 -0.3 104,741 - GFF88 0.62 +1.0 104,741 - GFF88 0.62 -0.1 104,746 + GFF88 0.62 -1.1 104,857 + GFF88 0.70 -1.3
Or see this region's nucleotide sequence