Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7259 and GFF7260 are separated by 30 nucleotides GFF7260 and GFF7261 are separated by 2 nucleotides GFF7261 and GFF7262 overlap by 4 nucleotides GFF7262 and GFF7263 are separated by 6 nucleotides
GFF7259 - no description, at 6,685 to 7,509
GFF7259
GFF7260 - hypothetical protein, at 7,540 to 7,989
GFF7260
GFF7261 - 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26), at 7,992 to 9,428
GFF7261
GFF7262 - no description, at 9,425 to 9,718
GFF7262
GFF7263 - Malate dehydrogenase (EC 1.1.1.37), at 9,725 to 10,819
GFF7263
Position (kb) 7
8
9
10 Strain fitness (log2 ratio) -2
-1
0
1
2 at 7.125 kb on + strand, within GFF7259 at 7.207 kb on + strand, within GFF7259 at 7.207 kb on + strand, within GFF7259 at 7.208 kb on - strand, within GFF7259 at 7.208 kb on - strand, within GFF7259 at 7.261 kb on + strand, within GFF7259 at 7.551 kb on + strand at 7.552 kb on - strand at 7.555 kb on + strand at 7.859 kb on - strand, within GFF7260 at 7.900 kb on + strand, within GFF7260 at 7.901 kb on - strand, within GFF7260 at 7.901 kb on - strand, within GFF7260 at 7.901 kb on - strand, within GFF7260 at 8.333 kb on + strand, within GFF7261 at 9.590 kb on + strand, within GFF7262 at 9.590 kb on + strand, within GFF7262 at 9.635 kb on + strand, within GFF7262 at 9.683 kb on + strand, within GFF7262 at 9.683 kb on + strand, within GFF7262 at 9.707 kb on + strand at 9.761 kb on + strand at 9.860 kb on + strand, within GFF7263 at 10.047 kb on - strand, within GFF7263 at 10.164 kb on - strand, within GFF7263 at 10.226 kb on - strand, within GFF7263
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 7,125 + GFF7259 0.53 -0.2 7,207 + GFF7259 0.63 -0.5 7,207 + GFF7259 0.63 +0.1 7,208 - GFF7259 0.63 +0.0 7,208 - GFF7259 0.63 -0.0 7,261 + GFF7259 0.70 +1.8 7,551 + +2.1 7,552 - -0.4 7,555 + -2.0 7,859 - GFF7260 0.71 -0.8 7,900 + GFF7260 0.80 -0.4 7,901 - GFF7260 0.80 +0.9 7,901 - GFF7260 0.80 -0.3 7,901 - GFF7260 0.80 -1.0 8,333 + GFF7261 0.24 -1.5 9,590 + GFF7262 0.56 -0.8 9,590 + GFF7262 0.56 -0.4 9,635 + GFF7262 0.71 -0.5 9,683 + GFF7262 0.88 -1.2 9,683 + GFF7262 0.88 -0.7 9,707 + -0.3 9,761 + -0.9 9,860 + GFF7263 0.12 +0.2 10,047 - GFF7263 0.29 +0.4 10,164 - GFF7263 0.40 -1.8 10,226 - GFF7263 0.46 +0.8
Or see this region's nucleotide sequence