Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7226 and GFF7227 are separated by 7 nucleotides GFF7227 and GFF7228 are separated by 175 nucleotides GFF7228 and GFF7229 are separated by 28 nucleotides
GFF7226 - hypothetical protein, at 7,496 to 8,335
GFF7226
GFF7227 - no description, at 8,343 to 8,612
GFF7227
GFF7228 - Ribonucleotide reductase of class II (coenzyme B12-dependent) (EC 1.17.4.1), at 8,788 to 10,638
GFF7228
GFF7229 - no description, at 10,667 to 11,926
GFF7229
Position (kb)
8
9
10
11 Strain fitness (log2 ratio)
-1
0
1
2 at 8.144 kb on + strand, within GFF7226 at 8.144 kb on + strand, within GFF7226 at 8.144 kb on + strand, within GFF7226 at 8.145 kb on - strand, within GFF7226 at 8.145 kb on - strand, within GFF7226 at 8.145 kb on - strand, within GFF7226 at 8.638 kb on + strand at 8.639 kb on - strand at 8.703 kb on + strand at 8.704 kb on - strand at 8.875 kb on + strand at 8.875 kb on + strand at 8.876 kb on - strand at 9.127 kb on + strand, within GFF7228 at 9.127 kb on + strand, within GFF7228 at 9.128 kb on - strand, within GFF7228 at 9.128 kb on - strand, within GFF7228 at 9.537 kb on - strand, within GFF7228 at 9.538 kb on + strand, within GFF7228 at 9.539 kb on - strand, within GFF7228 at 10.078 kb on + strand, within GFF7228 at 10.079 kb on - strand, within GFF7228 at 10.291 kb on + strand, within GFF7228 at 10.292 kb on - strand, within GFF7228 at 10.427 kb on - strand, within GFF7228 at 10.478 kb on - strand at 10.498 kb on + strand at 10.498 kb on + strand at 10.498 kb on + strand at 10.498 kb on + strand at 10.498 kb on + strand at 10.498 kb on + strand at 10.498 kb on + strand at 10.498 kb on + strand at 10.499 kb on - strand at 10.499 kb on - strand at 10.540 kb on + strand at 10.541 kb on - strand at 10.743 kb on + strand at 10.743 kb on + strand at 10.743 kb on + strand at 10.744 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 8,144 + GFF7226 0.77 -0.6 8,144 + GFF7226 0.77 -0.7 8,144 + GFF7226 0.77 +0.5 8,145 - GFF7226 0.77 +0.4 8,145 - GFF7226 0.77 +1.2 8,145 - GFF7226 0.77 -0.3 8,638 + -0.7 8,639 - +0.4 8,703 + +1.0 8,704 - +0.6 8,875 + +0.2 8,875 + -0.1 8,876 - +0.7 9,127 + GFF7228 0.18 +0.8 9,127 + GFF7228 0.18 +0.8 9,128 - GFF7228 0.18 -0.8 9,128 - GFF7228 0.18 -1.4 9,537 - GFF7228 0.40 +2.2 9,538 + GFF7228 0.41 +1.8 9,539 - GFF7228 0.41 -0.6 10,078 + GFF7228 0.70 -0.7 10,079 - GFF7228 0.70 -0.4 10,291 + GFF7228 0.81 +1.3 10,292 - GFF7228 0.81 +0.1 10,427 - GFF7228 0.89 +0.9 10,478 - +0.1 10,498 + +0.3 10,498 + +0.4 10,498 + +0.2 10,498 + -0.4 10,498 + -0.2 10,498 + +0.5 10,498 + +0.6 10,498 + -1.7 10,499 - +0.9 10,499 - +2.1 10,540 + +2.2 10,541 - -0.8 10,743 + -1.6 10,743 + +0.4 10,743 + -0.9 10,744 - -0.3
Or see this region's nucleotide sequence