Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7189 and GFF7190 are separated by 57 nucleotides GFF7190 and GFF7191 overlap by 4 nucleotides
GFF7189 - dioxygenase, TauD/TfdA family, at 6,110 to 7,027
GFF7189
GFF7190 - Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases, at 7,085 to 8,455
GFF7190
GFF7191 - Acyl-CoA dehydrogenase; probable dibenzothiophene desulfurization enzyme, at 8,452 to 9,633
GFF7191
Position (kb) 7
8
9 Strain fitness (log2 ratio) -1
0
1 at 6.350 kb on + strand, within GFF7189 at 6.651 kb on - strand, within GFF7189 at 6.836 kb on + strand, within GFF7189 at 6.893 kb on + strand, within GFF7189 at 6.893 kb on + strand, within GFF7189 at 6.894 kb on - strand, within GFF7189 at 6.895 kb on + strand, within GFF7189 at 6.895 kb on + strand, within GFF7189 at 7.455 kb on + strand, within GFF7190 at 7.455 kb on + strand, within GFF7190 at 7.456 kb on - strand, within GFF7190 at 7.595 kb on + strand, within GFF7190 at 7.596 kb on - strand, within GFF7190 at 7.658 kb on + strand, within GFF7190 at 7.658 kb on + strand, within GFF7190 at 7.659 kb on - strand, within GFF7190 at 7.659 kb on - strand, within GFF7190 at 7.703 kb on + strand, within GFF7190 at 7.704 kb on - strand, within GFF7190 at 7.704 kb on - strand, within GFF7190 at 7.976 kb on + strand, within GFF7190 at 8.905 kb on + strand, within GFF7191 at 8.905 kb on + strand, within GFF7191 at 8.905 kb on + strand, within GFF7191 at 8.906 kb on - strand, within GFF7191 at 8.981 kb on - strand, within GFF7191 at 8.981 kb on - strand, within GFF7191 at 8.981 kb on - strand, within GFF7191
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 6,350 + GFF7189 0.26 -0.3 6,651 - GFF7189 0.59 +0.5 6,836 + GFF7189 0.79 -1.0 6,893 + GFF7189 0.85 +0.7 6,893 + GFF7189 0.85 +0.2 6,894 - GFF7189 0.85 +0.5 6,895 + GFF7189 0.86 -0.2 6,895 + GFF7189 0.86 +0.2 7,455 + GFF7190 0.27 -0.4 7,455 + GFF7190 0.27 +0.2 7,456 - GFF7190 0.27 -0.6 7,595 + GFF7190 0.37 +0.3 7,596 - GFF7190 0.37 +0.1 7,658 + GFF7190 0.42 +1.3 7,658 + GFF7190 0.42 -0.4 7,659 - GFF7190 0.42 +0.1 7,659 - GFF7190 0.42 +0.8 7,703 + GFF7190 0.45 -0.1 7,704 - GFF7190 0.45 +0.1 7,704 - GFF7190 0.45 +1.3 7,976 + GFF7190 0.65 -0.1 8,905 + GFF7191 0.38 +0.1 8,905 + GFF7191 0.38 -0.2 8,905 + GFF7191 0.38 -0.7 8,906 - GFF7191 0.38 +0.1 8,981 - GFF7191 0.45 +0.1 8,981 - GFF7191 0.45 +0.3 8,981 - GFF7191 0.45 +0.6
Or see this region's nucleotide sequence