Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6944 and GFF6945 are separated by 101 nucleotides GFF6945 and GFF6946 overlap by 8 nucleotides GFF6946 and GFF6947 are separated by 21 nucleotides
GFF6944 - Xylulose-5-phosphate phosphoketolase (EC 4.1.2.9) @ Fructose-6-phosphate phosphoketolase (EC 4.1.2.22), at 18,689 to 21,061
GFF6944
GFF6945 - Two-component transcriptional response regulator, OmpR family, at 21,163 to 21,891
GFF6945
GFF6946 - Sensory box histidine kinase/response regulator, at 21,884 to 24,001
GFF6946
GFF6947 - transcriptional regulator, Crp/Fnr family, at 24,023 to 24,790
GFF6947
Position (kb) 21
22
23
24
25 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 21.002 kb on + strand at 21.002 kb on + strand at 21.002 kb on + strand at 21.002 kb on + strand at 21.002 kb on + strand at 21.003 kb on - strand at 21.003 kb on - strand at 21.003 kb on - strand at 21.003 kb on - strand at 21.003 kb on - strand at 21.083 kb on + strand at 21.083 kb on + strand at 21.084 kb on - strand at 21.106 kb on + strand at 21.106 kb on + strand at 21.130 kb on + strand at 21.130 kb on + strand at 21.146 kb on + strand at 21.146 kb on + strand at 21.147 kb on - strand at 21.147 kb on - strand at 21.207 kb on + strand at 21.208 kb on - strand at 21.229 kb on + strand at 21.230 kb on - strand at 21.230 kb on - strand at 21.449 kb on + strand, within GFF6945 at 21.449 kb on + strand, within GFF6945 at 21.450 kb on - strand, within GFF6945 at 21.450 kb on - strand, within GFF6945 at 21.450 kb on - strand, within GFF6945 at 21.463 kb on + strand, within GFF6945 at 21.859 kb on + strand at 21.936 kb on + strand at 21.937 kb on - strand at 21.937 kb on - strand at 22.068 kb on - strand at 22.068 kb on - strand at 22.142 kb on + strand, within GFF6946 at 22.155 kb on - strand, within GFF6946 at 22.346 kb on + strand, within GFF6946 at 22.616 kb on + strand, within GFF6946 at 22.652 kb on + strand, within GFF6946 at 22.652 kb on + strand, within GFF6946 at 22.653 kb on - strand, within GFF6946 at 23.099 kb on + strand, within GFF6946 at 23.099 kb on + strand, within GFF6946 at 23.099 kb on + strand, within GFF6946 at 23.100 kb on - strand, within GFF6946 at 23.475 kb on + strand, within GFF6946 at 23.475 kb on + strand, within GFF6946 at 23.477 kb on + strand, within GFF6946 at 23.477 kb on + strand, within GFF6946 at 23.477 kb on + strand, within GFF6946 at 23.478 kb on - strand, within GFF6946 at 23.478 kb on - strand, within GFF6946 at 23.478 kb on - strand, within GFF6946 at 23.553 kb on - strand, within GFF6946 at 23.914 kb on - strand at 23.945 kb on + strand at 23.945 kb on + strand at 24.025 kb on - strand at 24.193 kb on - strand, within GFF6947 at 24.193 kb on - strand, within GFF6947 at 24.274 kb on - strand, within GFF6947 at 24.274 kb on - strand, within GFF6947 at 24.475 kb on - strand, within GFF6947 at 24.595 kb on - strand, within GFF6947 at 24.595 kb on - strand, within GFF6947 at 24.625 kb on - strand, within GFF6947 at 24.791 kb on - strand at 24.832 kb on + strand at 24.832 kb on + strand at 24.833 kb on - strand at 24.877 kb on + strand at 24.909 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 21,002 + +1.8 21,002 + +0.8 21,002 + -0.8 21,002 + +1.4 21,002 + -0.7 21,003 - -0.1 21,003 - -0.5 21,003 - +0.1 21,003 - +0.6 21,003 - -1.2 21,083 + +0.1 21,083 + -1.2 21,084 - -2.9 21,106 + +0.2 21,106 + -0.5 21,130 + +1.3 21,130 + -0.6 21,146 + -0.7 21,146 + -0.4 21,147 - -0.2 21,147 - +0.2 21,207 + +0.3 21,208 - -0.0 21,229 + -2.0 21,230 - +0.0 21,230 - -1.8 21,449 + GFF6945 0.39 +0.1 21,449 + GFF6945 0.39 -0.3 21,450 - GFF6945 0.39 -0.0 21,450 - GFF6945 0.39 -1.4 21,450 - GFF6945 0.39 +0.2 21,463 + GFF6945 0.41 -0.4 21,859 + -0.5 21,936 + +1.0 21,937 - -0.6 21,937 - -0.7 22,068 - -1.0 22,068 - +0.9 22,142 + GFF6946 0.12 -0.5 22,155 - GFF6946 0.13 +0.4 22,346 + GFF6946 0.22 +0.1 22,616 + GFF6946 0.35 -2.3 22,652 + GFF6946 0.36 +0.7 22,652 + GFF6946 0.36 +1.8 22,653 - GFF6946 0.36 +0.4 23,099 + GFF6946 0.57 +0.4 23,099 + GFF6946 0.57 -1.6 23,099 + GFF6946 0.57 +0.3 23,100 - GFF6946 0.57 -0.7 23,475 + GFF6946 0.75 +0.2 23,475 + GFF6946 0.75 -1.9 23,477 + GFF6946 0.75 -0.5 23,477 + GFF6946 0.75 -0.3 23,477 + GFF6946 0.75 -2.1 23,478 - GFF6946 0.75 +1.8 23,478 - GFF6946 0.75 +1.1 23,478 - GFF6946 0.75 +2.0 23,553 - GFF6946 0.79 +1.4 23,914 - +2.8 23,945 + -0.7 23,945 + +1.0 24,025 - +0.8 24,193 - GFF6947 0.22 +0.4 24,193 - GFF6947 0.22 +0.4 24,274 - GFF6947 0.33 +0.3 24,274 - GFF6947 0.33 -0.0 24,475 - GFF6947 0.59 +0.2 24,595 - GFF6947 0.74 +1.2 24,595 - GFF6947 0.74 -0.1 24,625 - GFF6947 0.78 -0.6 24,791 - -0.8 24,832 + -0.2 24,832 + +0.2 24,833 - +3.0 24,877 + -0.9 24,909 + +0.3
Or see this region's nucleotide sequence