Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6731 and GFF6732 overlap by 4 nucleotides GFF6732 and GFF6733 are separated by 4 nucleotides GFF6733 and GFF6734 are separated by 220 nucleotides
GFF6731 - Nucleoside ABC transporter, permease protein 1, at 41,497 to 42,435
GFF6731
GFF6732 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 42,432 to 43,310
GFF6732
GFF6733 - hypothetical protein, at 43,315 to 44,517
GFF6733
GFF6734 - Transcriptional regulator, AraC family, at 44,738 to 45,694
GFF6734
Position (kb)
43
44
45 Strain fitness (log2 ratio)
-2
-1
0
1 at 42.490 kb on + strand at 42.490 kb on + strand at 42.491 kb on - strand at 42.523 kb on + strand, within GFF6732 at 42.526 kb on + strand, within GFF6732 at 42.526 kb on + strand, within GFF6732 at 42.526 kb on + strand, within GFF6732 at 42.526 kb on + strand, within GFF6732 at 42.526 kb on + strand, within GFF6732 at 42.527 kb on - strand, within GFF6732 at 42.527 kb on - strand, within GFF6732 at 42.527 kb on - strand, within GFF6732 at 42.527 kb on - strand, within GFF6732 at 42.632 kb on - strand, within GFF6732 at 42.632 kb on - strand, within GFF6732 at 42.662 kb on - strand, within GFF6732 at 43.219 kb on + strand, within GFF6732 at 43.253 kb on - strand at 43.253 kb on - strand at 43.453 kb on + strand, within GFF6733 at 43.453 kb on + strand, within GFF6733 at 43.453 kb on + strand, within GFF6733 at 43.454 kb on - strand, within GFF6733 at 43.616 kb on + strand, within GFF6733 at 43.700 kb on + strand, within GFF6733 at 43.701 kb on - strand, within GFF6733 at 43.701 kb on - strand, within GFF6733 at 43.736 kb on + strand, within GFF6733 at 43.737 kb on - strand, within GFF6733 at 44.336 kb on + strand, within GFF6733 at 44.975 kb on + strand, within GFF6734 at 44.976 kb on - strand, within GFF6734 at 45.065 kb on + strand, within GFF6734 at 45.065 kb on + strand, within GFF6734 at 45.066 kb on - strand, within GFF6734 at 45.083 kb on + strand, within GFF6734 at 45.084 kb on - strand, within GFF6734 at 45.242 kb on + strand, within GFF6734 at 45.242 kb on + strand, within GFF6734 at 45.510 kb on - strand, within GFF6734 at 45.510 kb on - strand, within GFF6734
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 42,490 + -1.9 42,490 + -2.1 42,491 - -0.9 42,523 + GFF6732 0.10 -0.7 42,526 + GFF6732 0.11 -0.1 42,526 + GFF6732 0.11 +0.3 42,526 + GFF6732 0.11 -0.6 42,526 + GFF6732 0.11 -1.7 42,526 + GFF6732 0.11 -0.6 42,527 - GFF6732 0.11 -0.1 42,527 - GFF6732 0.11 +0.0 42,527 - GFF6732 0.11 -0.5 42,527 - GFF6732 0.11 +0.1 42,632 - GFF6732 0.23 -0.7 42,632 - GFF6732 0.23 +0.2 42,662 - GFF6732 0.26 +1.4 43,219 + GFF6732 0.90 +0.2 43,253 - -0.4 43,253 - -1.6 43,453 + GFF6733 0.11 +0.3 43,453 + GFF6733 0.11 +1.1 43,453 + GFF6733 0.11 +0.6 43,454 - GFF6733 0.12 +0.5 43,616 + GFF6733 0.25 +1.6 43,700 + GFF6733 0.32 -0.9 43,701 - GFF6733 0.32 -0.5 43,701 - GFF6733 0.32 +0.4 43,736 + GFF6733 0.35 -0.7 43,737 - GFF6733 0.35 +0.8 44,336 + GFF6733 0.85 -0.3 44,975 + GFF6734 0.25 +0.6 44,976 - GFF6734 0.25 +0.5 45,065 + GFF6734 0.34 -1.5 45,065 + GFF6734 0.34 -0.7 45,066 - GFF6734 0.34 -0.6 45,083 + GFF6734 0.36 -0.3 45,084 - GFF6734 0.36 +0.6 45,242 + GFF6734 0.53 -1.2 45,242 + GFF6734 0.53 -0.8 45,510 - GFF6734 0.81 -2.2 45,510 - GFF6734 0.81 -1.2
Or see this region's nucleotide sequence