Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6714 and GFF6715 are separated by 10 nucleotides GFF6715 and GFF6716 overlap by 8 nucleotides
GFF6714 - Putative cryptic D-serine deaminase (EC 4.3.1.18), at 22,301 to 23,617
GFF6714
GFF6715 - Transcriptional regulator of RpiR family in catabolic operon, at 23,628 to 24,527
GFF6715
GFF6716 - N-acyl-D-amino-acid deacylase (EC 3.5.1.81), at 24,520 to 26,001
GFF6716
Position (kb)
23
24
25 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 22.666 kb on + strand, within GFF6714 at 22.822 kb on - strand, within GFF6714 at 22.822 kb on - strand, within GFF6714 at 22.822 kb on - strand, within GFF6714 at 22.952 kb on + strand, within GFF6714 at 22.952 kb on + strand, within GFF6714 at 22.952 kb on + strand, within GFF6714 at 22.998 kb on - strand, within GFF6714 at 22.998 kb on - strand, within GFF6714 at 23.177 kb on + strand, within GFF6714 at 23.177 kb on + strand, within GFF6714 at 23.177 kb on + strand, within GFF6714 at 23.178 kb on - strand, within GFF6714 at 23.178 kb on - strand, within GFF6714 at 23.178 kb on - strand, within GFF6714 at 23.457 kb on - strand, within GFF6714 at 23.663 kb on - strand at 24.034 kb on - strand, within GFF6715 at 24.123 kb on + strand, within GFF6715 at 24.123 kb on + strand, within GFF6715 at 24.123 kb on + strand, within GFF6715 at 24.124 kb on - strand, within GFF6715 at 24.354 kb on + strand, within GFF6715 at 24.354 kb on + strand, within GFF6715 at 24.382 kb on + strand, within GFF6715 at 24.382 kb on + strand, within GFF6715 at 25.117 kb on + strand, within GFF6716 at 25.118 kb on - strand, within GFF6716 at 25.118 kb on - strand, within GFF6716 at 25.192 kb on + strand, within GFF6716 at 25.390 kb on + strand, within GFF6716 at 25.390 kb on + strand, within GFF6716 at 25.391 kb on - strand, within GFF6716 at 25.391 kb on - strand, within GFF6716 at 25.391 kb on - strand, within GFF6716 at 25.391 kb on - strand, within GFF6716 at 25.396 kb on + strand, within GFF6716
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 22,666 + GFF6714 0.28 -0.4 22,822 - GFF6714 0.40 -0.5 22,822 - GFF6714 0.40 +1.3 22,822 - GFF6714 0.40 -0.5 22,952 + GFF6714 0.49 -0.8 22,952 + GFF6714 0.49 +0.1 22,952 + GFF6714 0.49 +0.0 22,998 - GFF6714 0.53 -0.8 22,998 - GFF6714 0.53 -1.7 23,177 + GFF6714 0.67 -0.0 23,177 + GFF6714 0.67 -0.8 23,177 + GFF6714 0.67 +2.1 23,178 - GFF6714 0.67 -1.9 23,178 - GFF6714 0.67 +0.6 23,178 - GFF6714 0.67 -0.7 23,457 - GFF6714 0.88 -2.1 23,663 - -1.1 24,034 - GFF6715 0.45 -0.1 24,123 + GFF6715 0.55 -0.2 24,123 + GFF6715 0.55 +0.6 24,123 + GFF6715 0.55 +0.1 24,124 - GFF6715 0.55 +1.4 24,354 + GFF6715 0.81 -0.4 24,354 + GFF6715 0.81 -1.8 24,382 + GFF6715 0.84 -0.5 24,382 + GFF6715 0.84 -0.5 25,117 + GFF6716 0.40 +0.4 25,118 - GFF6716 0.40 -2.2 25,118 - GFF6716 0.40 +1.2 25,192 + GFF6716 0.45 +0.4 25,390 + GFF6716 0.59 -0.7 25,390 + GFF6716 0.59 +0.5 25,391 - GFF6716 0.59 -1.0 25,391 - GFF6716 0.59 +0.9 25,391 - GFF6716 0.59 -0.1 25,391 - GFF6716 0.59 +0.1 25,396 + GFF6716 0.59 +0.6
Or see this region's nucleotide sequence