Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6002 and GFF6003 are separated by 62 nucleotides GFF6003 and GFF6004 are separated by 66 nucleotides GFF6004 and GFF6005 are separated by 112 nucleotides
GFF6002 - DNA topoisomerase IB (poxvirus type) (EC 5.99.1.2), at 60,561 to 61,811
GFF6002
GFF6003 - no description, at 61,874 to 62,488
GFF6003
GFF6004 - hypothetical protein, at 62,555 to 62,734
GFF6004
GFF6005 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 62,847 to 64,352
GFF6005
Position (kb) 61
62
63 Strain fitness (log2 ratio) -2
-1
0
1
2 at 60.874 kb on - strand, within GFF6002 at 61.110 kb on + strand, within GFF6002 at 61.279 kb on - strand, within GFF6002 at 61.332 kb on + strand, within GFF6002 at 61.332 kb on + strand, within GFF6002 at 61.536 kb on + strand, within GFF6002 at 61.536 kb on + strand, within GFF6002 at 61.536 kb on + strand, within GFF6002 at 61.536 kb on + strand, within GFF6002 at 61.536 kb on + strand, within GFF6002 at 61.536 kb on + strand, within GFF6002 at 61.536 kb on + strand, within GFF6002 at 61.536 kb on + strand, within GFF6002 at 61.536 kb on + strand, within GFF6002 at 61.537 kb on - strand, within GFF6002 at 61.537 kb on - strand, within GFF6002 at 61.537 kb on - strand, within GFF6002 at 61.537 kb on - strand, within GFF6002 at 61.537 kb on - strand, within GFF6002 at 61.537 kb on - strand, within GFF6002 at 61.656 kb on + strand, within GFF6002 at 61.657 kb on - strand, within GFF6002 at 61.657 kb on - strand, within GFF6002 at 61.657 kb on - strand, within GFF6002 at 61.809 kb on + strand at 62.019 kb on + strand, within GFF6003 at 62.020 kb on - strand, within GFF6003 at 62.020 kb on - strand, within GFF6003 at 62.479 kb on + strand at 62.479 kb on + strand at 62.508 kb on + strand at 62.508 kb on + strand at 62.508 kb on + strand at 62.509 kb on - strand at 62.509 kb on - strand at 62.509 kb on - strand at 62.509 kb on - strand at 62.509 kb on - strand at 62.535 kb on + strand at 62.536 kb on - strand at 62.536 kb on - strand at 62.548 kb on - strand at 62.571 kb on + strand at 62.571 kb on + strand at 62.572 kb on - strand at 62.572 kb on - strand at 62.658 kb on + strand, within GFF6004 at 62.848 kb on + strand at 62.948 kb on - strand at 63.272 kb on - strand, within GFF6005 at 63.303 kb on + strand, within GFF6005 at 63.303 kb on + strand, within GFF6005 at 63.303 kb on + strand, within GFF6005 at 63.304 kb on - strand, within GFF6005 at 63.304 kb on - strand, within GFF6005 at 63.304 kb on - strand, within GFF6005 at 63.304 kb on - strand, within GFF6005 at 63.304 kb on - strand, within GFF6005 at 63.304 kb on - strand, within GFF6005 at 63.440 kb on - strand, within GFF6005 at 63.440 kb on - strand, within GFF6005 at 63.455 kb on - strand, within GFF6005
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 60,874 - GFF6002 0.25 +0.7 61,110 + GFF6002 0.44 -0.3 61,279 - GFF6002 0.57 -1.2 61,332 + GFF6002 0.62 -1.5 61,332 + GFF6002 0.62 +0.8 61,536 + GFF6002 0.78 -1.4 61,536 + GFF6002 0.78 -0.3 61,536 + GFF6002 0.78 -0.8 61,536 + GFF6002 0.78 -1.1 61,536 + GFF6002 0.78 +0.4 61,536 + GFF6002 0.78 +2.4 61,536 + GFF6002 0.78 -0.2 61,536 + GFF6002 0.78 +0.2 61,536 + GFF6002 0.78 -1.8 61,537 - GFF6002 0.78 +0.9 61,537 - GFF6002 0.78 +0.9 61,537 - GFF6002 0.78 -1.5 61,537 - GFF6002 0.78 -0.3 61,537 - GFF6002 0.78 -0.1 61,537 - GFF6002 0.78 -0.2 61,656 + GFF6002 0.88 +0.2 61,657 - GFF6002 0.88 -0.5 61,657 - GFF6002 0.88 -0.5 61,657 - GFF6002 0.88 -0.2 61,809 + +0.5 62,019 + GFF6003 0.24 -2.1 62,020 - GFF6003 0.24 -1.0 62,020 - GFF6003 0.24 -2.0 62,479 + -0.7 62,479 + +1.0 62,508 + -0.3 62,508 + -0.3 62,508 + -0.3 62,509 - -0.2 62,509 - +0.3 62,509 - -0.2 62,509 - -0.5 62,509 - +1.9 62,535 + -1.2 62,536 - +1.2 62,536 - -0.5 62,548 - -1.4 62,571 + +0.4 62,571 + -1.5 62,572 - -0.2 62,572 - +0.5 62,658 + GFF6004 0.57 +0.6 62,848 + -1.5 62,948 - -1.1 63,272 - GFF6005 0.28 -1.0 63,303 + GFF6005 0.30 -0.2 63,303 + GFF6005 0.30 +1.2 63,303 + GFF6005 0.30 -0.1 63,304 - GFF6005 0.30 -1.1 63,304 - GFF6005 0.30 +0.5 63,304 - GFF6005 0.30 -0.5 63,304 - GFF6005 0.30 -1.1 63,304 - GFF6005 0.30 -0.9 63,304 - GFF6005 0.30 +0.6 63,440 - GFF6005 0.39 -0.9 63,440 - GFF6005 0.39 -0.8 63,455 - GFF6005 0.40 -0.1
Or see this region's nucleotide sequence