Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5754 and GFF5755 are separated by 40 nucleotides GFF5755 and GFF5756 are separated by 40 nucleotides GFF5756 and GFF5757 are separated by 29 nucleotides
GFF5754 - Flagellar cap protein FliD, at 127,402 to 128,847
GFF5754
GFF5755 - Flagellar biosynthesis protein FliS, at 128,888 to 129,307
GFF5755
GFF5756 - KEGG: flagellar protein FliT, at 129,348 to 129,680
GFF5756
GFF5757 - hypothetical protein, at 129,710 to 131,014
GFF5757
Position (kb)
129
130 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 128.597 kb on - strand, within GFF5754 at 128.698 kb on + strand, within GFF5754 at 128.698 kb on + strand, within GFF5754 at 128.800 kb on + strand at 128.801 kb on - strand at 128.845 kb on + strand at 128.845 kb on + strand at 128.846 kb on - strand at 128.846 kb on - strand at 128.891 kb on + strand at 128.891 kb on + strand at 128.891 kb on + strand at 128.891 kb on + strand at 128.891 kb on + strand at 128.892 kb on - strand at 128.892 kb on - strand at 128.892 kb on - strand at 128.892 kb on - strand at 128.892 kb on - strand at 128.892 kb on - strand at 128.892 kb on - strand at 128.892 kb on - strand at 128.892 kb on - strand at 129.176 kb on + strand, within GFF5755 at 129.177 kb on - strand, within GFF5755 at 129.177 kb on - strand, within GFF5755 at 129.182 kb on + strand, within GFF5755 at 129.182 kb on + strand, within GFF5755 at 129.182 kb on + strand, within GFF5755 at 129.182 kb on + strand, within GFF5755 at 129.183 kb on - strand, within GFF5755 at 129.183 kb on - strand, within GFF5755 at 129.183 kb on - strand, within GFF5755 at 129.353 kb on - strand at 129.353 kb on - strand at 129.379 kb on - strand at 129.630 kb on + strand, within GFF5756 at 129.631 kb on - strand, within GFF5756 at 129.631 kb on - strand, within GFF5756 at 129.631 kb on - strand, within GFF5756 at 129.631 kb on - strand, within GFF5756 at 129.631 kb on - strand, within GFF5756 at 129.664 kb on - strand at 130.115 kb on + strand, within GFF5757 at 130.115 kb on + strand, within GFF5757 at 130.115 kb on + strand, within GFF5757 at 130.116 kb on - strand, within GFF5757 at 130.116 kb on - strand, within GFF5757 at 130.116 kb on - strand, within GFF5757 at 130.116 kb on - strand, within GFF5757 at 130.429 kb on + strand, within GFF5757 at 130.429 kb on + strand, within GFF5757 at 130.429 kb on + strand, within GFF5757 at 130.430 kb on - strand, within GFF5757 at 130.496 kb on + strand, within GFF5757 at 130.497 kb on - strand, within GFF5757 at 130.497 kb on - strand, within GFF5757 at 130.497 kb on - strand, within GFF5757 at 130.497 kb on - strand, within GFF5757
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 128,597 - GFF5754 0.83 +0.0 128,698 + GFF5754 0.90 -0.9 128,698 + GFF5754 0.90 -0.1 128,800 + +0.5 128,801 - -1.0 128,845 + -1.9 128,845 + -0.3 128,846 - -0.8 128,846 - -0.6 128,891 + -0.5 128,891 + +3.0 128,891 + -1.8 128,891 + -0.1 128,891 + -0.9 128,892 - +0.2 128,892 - -0.9 128,892 - -1.1 128,892 - -0.1 128,892 - -1.8 128,892 - -0.7 128,892 - -0.4 128,892 - +0.7 128,892 - +0.7 129,176 + GFF5755 0.69 -0.1 129,177 - GFF5755 0.69 -0.6 129,177 - GFF5755 0.69 -0.7 129,182 + GFF5755 0.70 +0.2 129,182 + GFF5755 0.70 -1.8 129,182 + GFF5755 0.70 -0.9 129,182 + GFF5755 0.70 -1.1 129,183 - GFF5755 0.70 -1.0 129,183 - GFF5755 0.70 -0.3 129,183 - GFF5755 0.70 -0.4 129,353 - -0.4 129,353 - -0.4 129,379 - -0.5 129,630 + GFF5756 0.85 -0.3 129,631 - GFF5756 0.85 -0.8 129,631 - GFF5756 0.85 -1.8 129,631 - GFF5756 0.85 -0.3 129,631 - GFF5756 0.85 -1.1 129,631 - GFF5756 0.85 -0.9 129,664 - +1.4 130,115 + GFF5757 0.31 -0.2 130,115 + GFF5757 0.31 -0.3 130,115 + GFF5757 0.31 -0.1 130,116 - GFF5757 0.31 +0.5 130,116 - GFF5757 0.31 +0.9 130,116 - GFF5757 0.31 +0.6 130,116 - GFF5757 0.31 -1.8 130,429 + GFF5757 0.55 -0.5 130,429 + GFF5757 0.55 -0.4 130,429 + GFF5757 0.55 +1.1 130,430 - GFF5757 0.55 -0.1 130,496 + GFF5757 0.60 -1.3 130,497 - GFF5757 0.60 -0.4 130,497 - GFF5757 0.60 +0.7 130,497 - GFF5757 0.60 +0.3 130,497 - GFF5757 0.60 -0.9
Or see this region's nucleotide sequence