Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5473 and GFF5474 are separated by 4 nucleotides GFF5474 and GFF5475 are separated by 53 nucleotides GFF5475 and GFF5476 are separated by 42 nucleotides GFF5476 and GFF5477 are separated by 87 nucleotides
GFF5473 - hypothetical protein, at 101,940 to 104,396
GFF5473
GFF5474 - no description, at 104,401 to 104,607
GFF5474
GFF5475 - Uncharacterized MFS-type transporter, at 104,661 to 106,286
GFF5475
GFF5476 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 106,329 to 107,099
GFF5476
GFF5477 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 107,187 to 107,951
GFF5477
Position (kb)
104
105
106
107 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 103.777 kb on + strand, within GFF5473 at 103.778 kb on - strand, within GFF5473 at 103.778 kb on - strand, within GFF5473 at 103.919 kb on + strand, within GFF5473 at 103.919 kb on + strand, within GFF5473 at 103.960 kb on + strand, within GFF5473 at 103.961 kb on - strand, within GFF5473 at 103.961 kb on - strand, within GFF5473 at 103.961 kb on - strand, within GFF5473 at 103.961 kb on - strand, within GFF5473 at 103.961 kb on - strand, within GFF5473 at 103.961 kb on - strand, within GFF5473 at 104.015 kb on + strand, within GFF5473 at 104.015 kb on + strand, within GFF5473 at 104.015 kb on + strand, within GFF5473 at 104.015 kb on + strand, within GFF5473 at 104.016 kb on - strand, within GFF5473 at 104.016 kb on - strand, within GFF5473 at 104.016 kb on - strand, within GFF5473 at 104.016 kb on - strand, within GFF5473 at 104.016 kb on - strand, within GFF5473 at 104.210 kb on + strand at 104.210 kb on + strand at 104.417 kb on + strand at 104.417 kb on + strand at 104.417 kb on + strand at 104.417 kb on + strand at 104.418 kb on - strand at 104.418 kb on - strand at 104.418 kb on - strand at 104.418 kb on - strand at 104.418 kb on - strand at 104.534 kb on + strand, within GFF5474 at 104.534 kb on + strand, within GFF5474 at 104.534 kb on + strand, within GFF5474 at 104.535 kb on - strand, within GFF5474 at 104.560 kb on + strand, within GFF5474 at 104.560 kb on + strand, within GFF5474 at 104.560 kb on + strand, within GFF5474 at 104.560 kb on + strand, within GFF5474 at 104.677 kb on + strand at 104.677 kb on + strand at 104.678 kb on - strand at 104.812 kb on + strand at 104.812 kb on + strand at 104.812 kb on + strand at 104.812 kb on + strand at 104.812 kb on + strand at 104.813 kb on - strand at 104.813 kb on - strand at 104.814 kb on - strand at 105.184 kb on + strand, within GFF5475 at 105.184 kb on + strand, within GFF5475 at 105.184 kb on + strand, within GFF5475 at 105.185 kb on - strand, within GFF5475 at 105.185 kb on - strand, within GFF5475 at 106.256 kb on + strand at 106.257 kb on - strand at 106.294 kb on + strand at 106.405 kb on + strand at 106.405 kb on + strand at 106.405 kb on + strand at 106.405 kb on + strand at 106.405 kb on + strand at 106.405 kb on + strand at 106.406 kb on - strand, within GFF5476 at 106.406 kb on - strand, within GFF5476 at 106.406 kb on - strand, within GFF5476 at 106.406 kb on - strand, within GFF5476 at 106.406 kb on - strand, within GFF5476 at 106.406 kb on - strand, within GFF5476 at 106.426 kb on + strand, within GFF5476 at 106.426 kb on + strand, within GFF5476 at 106.426 kb on + strand, within GFF5476 at 106.427 kb on - strand, within GFF5476 at 106.427 kb on - strand, within GFF5476 at 106.427 kb on - strand, within GFF5476 at 106.438 kb on + strand, within GFF5476 at 106.448 kb on - strand, within GFF5476 at 106.448 kb on - strand, within GFF5476 at 106.448 kb on - strand, within GFF5476 at 106.474 kb on + strand, within GFF5476 at 106.475 kb on - strand, within GFF5476 at 106.588 kb on + strand, within GFF5476 at 106.588 kb on + strand, within GFF5476 at 106.588 kb on + strand, within GFF5476 at 106.589 kb on - strand, within GFF5476 at 106.589 kb on - strand, within GFF5476 at 106.687 kb on + strand, within GFF5476 at 106.687 kb on + strand, within GFF5476 at 106.687 kb on + strand, within GFF5476 at 106.687 kb on + strand, within GFF5476 at 106.687 kb on + strand, within GFF5476 at 106.687 kb on + strand, within GFF5476 at 106.687 kb on + strand, within GFF5476 at 106.687 kb on + strand, within GFF5476 at 106.687 kb on + strand, within GFF5476 at 106.687 kb on + strand, within GFF5476 at 106.687 kb on + strand, within GFF5476 at 106.688 kb on - strand, within GFF5476 at 106.688 kb on - strand, within GFF5476 at 106.688 kb on - strand, within GFF5476 at 106.688 kb on - strand, within GFF5476 at 106.688 kb on - strand, within GFF5476 at 106.688 kb on - strand, within GFF5476 at 106.688 kb on - strand, within GFF5476 at 106.688 kb on - strand, within GFF5476 at 106.688 kb on - strand, within GFF5476 at 106.873 kb on + strand, within GFF5476 at 106.873 kb on + strand, within GFF5476 at 106.873 kb on + strand, within GFF5476 at 106.873 kb on + strand, within GFF5476 at 106.873 kb on + strand, within GFF5476 at 106.873 kb on + strand, within GFF5476 at 106.873 kb on + strand, within GFF5476 at 106.873 kb on + strand, within GFF5476 at 106.873 kb on + strand, within GFF5476 at 106.873 kb on + strand, within GFF5476 at 106.873 kb on + strand, within GFF5476 at 106.873 kb on + strand, within GFF5476 at 106.873 kb on + strand, within GFF5476 at 106.874 kb on - strand, within GFF5476 at 106.874 kb on - strand, within GFF5476 at 106.874 kb on - strand, within GFF5476 at 106.874 kb on - strand, within GFF5476 at 106.874 kb on - strand, within GFF5476 at 106.874 kb on - strand, within GFF5476 at 106.874 kb on - strand, within GFF5476 at 106.874 kb on - strand, within GFF5476 at 106.874 kb on - strand, within GFF5476 at 106.874 kb on - strand, within GFF5476 at 106.874 kb on - strand, within GFF5476 at 107.044 kb on + strand at 107.044 kb on + strand at 107.044 kb on + strand at 107.044 kb on + strand at 107.044 kb on + strand at 107.045 kb on - strand at 107.045 kb on - strand at 107.045 kb on - strand at 107.045 kb on - strand at 107.045 kb on - strand at 107.045 kb on - strand at 107.045 kb on - strand at 107.045 kb on - strand at 107.045 kb on - strand at 107.045 kb on - strand at 107.103 kb on + strand at 107.103 kb on + strand at 107.103 kb on + strand at 107.103 kb on + strand at 107.103 kb on + strand at 107.103 kb on + strand at 107.103 kb on + strand at 107.103 kb on + strand at 107.103 kb on + strand at 107.103 kb on + strand at 107.103 kb on + strand at 107.103 kb on + strand at 107.103 kb on + strand at 107.103 kb on + strand at 107.103 kb on + strand at 107.103 kb on + strand at 107.103 kb on + strand at 107.103 kb on + strand at 107.103 kb on + strand at 107.104 kb on - strand at 107.104 kb on - strand at 107.104 kb on - strand at 107.104 kb on - strand at 107.104 kb on - strand at 107.104 kb on - strand at 107.104 kb on - strand at 107.104 kb on - strand at 107.104 kb on - strand at 107.104 kb on - strand at 107.104 kb on - strand at 107.104 kb on - strand at 107.104 kb on - strand at 107.104 kb on - strand at 107.104 kb on - strand at 107.104 kb on - strand at 107.104 kb on - strand at 107.224 kb on + strand at 107.224 kb on + strand at 107.224 kb on + strand at 107.224 kb on + strand at 107.224 kb on + strand at 107.224 kb on + strand at 107.224 kb on - strand at 107.225 kb on - strand at 107.225 kb on - strand at 107.225 kb on - strand at 107.225 kb on - strand at 107.225 kb on - strand at 107.225 kb on - strand at 107.225 kb on - strand at 107.225 kb on - strand at 107.225 kb on - strand at 107.225 kb on - strand at 107.225 kb on - strand at 107.236 kb on + strand at 107.236 kb on + strand at 107.236 kb on + strand at 107.236 kb on + strand at 107.236 kb on + strand at 107.236 kb on + strand at 107.236 kb on + strand at 107.236 kb on + strand at 107.237 kb on - strand at 107.237 kb on - strand at 107.237 kb on - strand at 107.237 kb on - strand at 107.237 kb on - strand at 107.237 kb on - strand at 107.237 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 103,777 + GFF5473 0.75 +0.1 103,778 - GFF5473 0.75 -0.7 103,778 - GFF5473 0.75 -0.5 103,919 + GFF5473 0.81 -0.6 103,919 + GFF5473 0.81 -0.2 103,960 + GFF5473 0.82 -0.4 103,961 - GFF5473 0.82 -1.1 103,961 - GFF5473 0.82 -0.1 103,961 - GFF5473 0.82 -0.1 103,961 - GFF5473 0.82 -0.3 103,961 - GFF5473 0.82 -0.3 103,961 - GFF5473 0.82 +0.0 104,015 + GFF5473 0.84 +0.2 104,015 + GFF5473 0.84 +0.1 104,015 + GFF5473 0.84 -0.2 104,015 + GFF5473 0.84 -0.2 104,016 - GFF5473 0.84 -0.8 104,016 - GFF5473 0.84 -0.7 104,016 - GFF5473 0.84 +0.1 104,016 - GFF5473 0.84 +0.6 104,016 - GFF5473 0.84 +0.6 104,210 + -0.4 104,210 + +0.0 104,417 + -0.6 104,417 + +0.3 104,417 + -1.1 104,417 + +2.6 104,418 - -0.6 104,418 - -1.1 104,418 - -0.8 104,418 - -0.1 104,418 - -0.6 104,534 + GFF5474 0.64 -0.3 104,534 + GFF5474 0.64 +0.0 104,534 + GFF5474 0.64 -0.4 104,535 - GFF5474 0.65 +0.6 104,560 + GFF5474 0.77 -0.9 104,560 + GFF5474 0.77 -0.1 104,560 + GFF5474 0.77 -0.7 104,560 + GFF5474 0.77 +0.7 104,677 + +0.4 104,677 + -0.3 104,678 - +0.1 104,812 + -1.2 104,812 + +0.0 104,812 + +0.7 104,812 + +0.0 104,812 + -1.1 104,813 - -0.6 104,813 - +0.7 104,814 - -0.5 105,184 + GFF5475 0.32 -1.7 105,184 + GFF5475 0.32 -0.1 105,184 + GFF5475 0.32 -1.0 105,185 - GFF5475 0.32 -1.0 105,185 - GFF5475 0.32 -0.2 106,256 + -0.8 106,257 - +0.7 106,294 + -1.3 106,405 + +1.0 106,405 + +0.3 106,405 + +2.1 106,405 + -0.1 106,405 + +0.5 106,405 + +0.3 106,406 - GFF5476 0.10 -1.1 106,406 - GFF5476 0.10 -1.0 106,406 - GFF5476 0.10 -1.0 106,406 - GFF5476 0.10 -1.1 106,406 - GFF5476 0.10 +0.3 106,406 - GFF5476 0.10 +0.2 106,426 + GFF5476 0.13 -1.0 106,426 + GFF5476 0.13 -1.3 106,426 + GFF5476 0.13 +0.3 106,427 - GFF5476 0.13 -0.5 106,427 - GFF5476 0.13 -0.4 106,427 - GFF5476 0.13 -0.3 106,438 + GFF5476 0.14 +1.9 106,448 - GFF5476 0.15 +0.1 106,448 - GFF5476 0.15 +3.2 106,448 - GFF5476 0.15 -0.2 106,474 + GFF5476 0.19 -0.5 106,475 - GFF5476 0.19 -0.1 106,588 + GFF5476 0.34 +1.1 106,588 + GFF5476 0.34 -0.7 106,588 + GFF5476 0.34 +0.0 106,589 - GFF5476 0.34 -0.7 106,589 - GFF5476 0.34 -0.2 106,687 + GFF5476 0.46 +0.0 106,687 + GFF5476 0.46 +0.4 106,687 + GFF5476 0.46 -0.2 106,687 + GFF5476 0.46 -1.3 106,687 + GFF5476 0.46 -0.3 106,687 + GFF5476 0.46 -0.7 106,687 + GFF5476 0.46 -1.0 106,687 + GFF5476 0.46 +0.6 106,687 + GFF5476 0.46 -0.2 106,687 + GFF5476 0.46 -0.3 106,687 + GFF5476 0.46 -0.3 106,688 - GFF5476 0.47 -1.3 106,688 - GFF5476 0.47 +2.6 106,688 - GFF5476 0.47 -0.7 106,688 - GFF5476 0.47 +0.2 106,688 - GFF5476 0.47 +0.2 106,688 - GFF5476 0.47 -0.4 106,688 - GFF5476 0.47 -0.4 106,688 - GFF5476 0.47 +0.4 106,688 - GFF5476 0.47 +0.3 106,873 + GFF5476 0.71 +1.4 106,873 + GFF5476 0.71 -0.4 106,873 + GFF5476 0.71 +0.5 106,873 + GFF5476 0.71 -0.6 106,873 + GFF5476 0.71 -1.3 106,873 + GFF5476 0.71 -1.3 106,873 + GFF5476 0.71 -0.1 106,873 + GFF5476 0.71 -1.2 106,873 + GFF5476 0.71 -0.7 106,873 + GFF5476 0.71 -1.8 106,873 + GFF5476 0.71 +1.9 106,873 + GFF5476 0.71 -0.1 106,873 + GFF5476 0.71 +1.4 106,874 - GFF5476 0.71 +0.9 106,874 - GFF5476 0.71 +1.4 106,874 - GFF5476 0.71 -0.1 106,874 - GFF5476 0.71 -0.6 106,874 - GFF5476 0.71 +0.7 106,874 - GFF5476 0.71 +0.4 106,874 - GFF5476 0.71 +0.2 106,874 - GFF5476 0.71 -0.1 106,874 - GFF5476 0.71 -1.3 106,874 - GFF5476 0.71 -1.0 106,874 - GFF5476 0.71 +0.5 107,044 + -0.7 107,044 + +0.4 107,044 + -1.5 107,044 + -0.2 107,044 + +0.0 107,045 - -0.6 107,045 - -0.8 107,045 - +0.1 107,045 - +1.3 107,045 - -0.1 107,045 - -0.2 107,045 - +0.3 107,045 - +0.4 107,045 - +0.5 107,045 - +0.0 107,103 + -0.0 107,103 + +0.9 107,103 + -0.0 107,103 + -1.0 107,103 + -1.1 107,103 + -0.3 107,103 + +1.9 107,103 + +0.3 107,103 + -1.8 107,103 + -0.4 107,103 + +0.4 107,103 + -1.1 107,103 + -0.0 107,103 + -1.4 107,103 + -0.9 107,103 + +1.5 107,103 + -0.5 107,103 + +0.4 107,103 + -0.7 107,104 - -0.6 107,104 - +0.0 107,104 - -0.1 107,104 - -0.4 107,104 - +0.3 107,104 - -0.1 107,104 - +0.5 107,104 - -2.5 107,104 - -1.0 107,104 - -1.0 107,104 - +0.5 107,104 - +0.4 107,104 - +1.2 107,104 - +0.8 107,104 - +0.0 107,104 - -0.2 107,104 - +0.5 107,224 + -0.0 107,224 + +0.3 107,224 + +0.5 107,224 + -0.3 107,224 + -1.3 107,224 + -1.3 107,224 - -0.6 107,225 - -1.0 107,225 - +0.1 107,225 - -0.1 107,225 - -0.2 107,225 - -0.5 107,225 - -0.1 107,225 - +0.0 107,225 - +0.5 107,225 - -1.1 107,225 - -0.7 107,225 - -0.6 107,236 + -0.1 107,236 + +1.9 107,236 + -0.1 107,236 + +0.6 107,236 + +0.4 107,236 + -0.7 107,236 + -0.9 107,236 + -0.6 107,237 - -0.4 107,237 - +0.0 107,237 - +0.3 107,237 - -0.5 107,237 - +0.3 107,237 - -0.2 107,237 - -0.5
Or see this region's nucleotide sequence