Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5215 and GFF5216 are separated by 97 nucleotides GFF5216 and GFF5217 are separated by 249 nucleotides GFF5217 and GFF5218 are separated by 25 nucleotides
GFF5215 - Efflux ABC transporter, permease/ATP-binding protein, at 77,432 to 79,234
GFF5215
GFF5216 - Metal dependent phosphohydrolase, at 79,332 to 79,892
GFF5216
GFF5217 - Ferric iron ABC transporter, iron-binding protein, at 80,142 to 81,176
GFF5217
GFF5218 - ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines), at 81,202 to 82,281
GFF5218
Position (kb)
80
81
82 Strain fitness (log2 ratio)
-2
-1
0
1 at 79.153 kb on - strand at 79.567 kb on + strand, within GFF5216 at 79.568 kb on - strand, within GFF5216 at 79.568 kb on - strand, within GFF5216 at 79.649 kb on - strand, within GFF5216 at 79.649 kb on - strand, within GFF5216 at 79.649 kb on - strand, within GFF5216 at 79.649 kb on - strand, within GFF5216 at 79.649 kb on - strand, within GFF5216 at 79.649 kb on - strand, within GFF5216 at 79.649 kb on - strand, within GFF5216 at 79.679 kb on + strand, within GFF5216 at 79.679 kb on + strand, within GFF5216 at 80.026 kb on - strand at 80.026 kb on - strand at 80.026 kb on - strand at 80.073 kb on - strand at 80.239 kb on - strand at 80.272 kb on - strand, within GFF5217 at 80.314 kb on - strand, within GFF5217 at 80.619 kb on + strand, within GFF5217 at 80.619 kb on + strand, within GFF5217 at 80.668 kb on - strand, within GFF5217 at 80.668 kb on - strand, within GFF5217 at 80.754 kb on + strand, within GFF5217 at 80.754 kb on + strand, within GFF5217 at 80.754 kb on + strand, within GFF5217 at 80.754 kb on + strand, within GFF5217 at 80.754 kb on + strand, within GFF5217 at 80.754 kb on + strand, within GFF5217 at 80.755 kb on - strand, within GFF5217 at 80.755 kb on - strand, within GFF5217 at 80.994 kb on + strand, within GFF5217 at 80.994 kb on + strand, within GFF5217 at 80.994 kb on + strand, within GFF5217 at 81.145 kb on - strand at 81.461 kb on - strand, within GFF5218 at 81.502 kb on + strand, within GFF5218 at 81.502 kb on - strand, within GFF5218 at 81.503 kb on - strand, within GFF5218 at 81.599 kb on - strand, within GFF5218 at 81.875 kb on - strand, within GFF5218
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 79,153 - -1.1 79,567 + GFF5216 0.42 +0.5 79,568 - GFF5216 0.42 +0.1 79,568 - GFF5216 0.42 -0.0 79,649 - GFF5216 0.57 +0.7 79,649 - GFF5216 0.57 -1.3 79,649 - GFF5216 0.57 -1.1 79,649 - GFF5216 0.57 +0.3 79,649 - GFF5216 0.57 +0.5 79,649 - GFF5216 0.57 -0.1 79,649 - GFF5216 0.57 -0.0 79,679 + GFF5216 0.62 -0.7 79,679 + GFF5216 0.62 -0.4 80,026 - -1.3 80,026 - -0.7 80,026 - +0.6 80,073 - +0.7 80,239 - -0.3 80,272 - GFF5217 0.13 -1.2 80,314 - GFF5217 0.17 +0.6 80,619 + GFF5217 0.46 -0.8 80,619 + GFF5217 0.46 -0.7 80,668 - GFF5217 0.51 -0.9 80,668 - GFF5217 0.51 +0.0 80,754 + GFF5217 0.59 -1.8 80,754 + GFF5217 0.59 -0.9 80,754 + GFF5217 0.59 -0.7 80,754 + GFF5217 0.59 +0.5 80,754 + GFF5217 0.59 -0.3 80,754 + GFF5217 0.59 -1.0 80,755 - GFF5217 0.59 -0.1 80,755 - GFF5217 0.59 -1.6 80,994 + GFF5217 0.82 -0.1 80,994 + GFF5217 0.82 -0.3 80,994 + GFF5217 0.82 -0.3 81,145 - +0.3 81,461 - GFF5218 0.24 -0.3 81,502 + GFF5218 0.28 +0.0 81,502 - GFF5218 0.28 -0.5 81,503 - GFF5218 0.28 -0.7 81,599 - GFF5218 0.37 +0.0 81,875 - GFF5218 0.62 -0.9
Or see this region's nucleotide sequence