Strain Fitness in Variovorax sp. SCN45 around GFF497

Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF496 and GFF497 overlap by 4 nucleotidesGFF497 and GFF498 are separated by 127 nucleotides GFF496 - Squalene synthase (EC 2.5.1.21), at 540,244 to 541,158 GFF496 GFF497 - Phytoene synthase (EC 2.5.1.32), at 541,155 to 541,994 GFF497 GFF498 - Squalene/phytoene desaturase HopC, at 542,122 to 543,378 GFF498 Position (kb) 541 542Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 540.206 kb on + strandat 540.207 kb on - strandat 540.207 kb on - strandat 540.283 kb on + strandat 540.283 kb on + strandat 540.283 kb on + strandat 540.284 kb on - strandat 540.284 kb on - strandat 540.349 kb on + strand, within GFF496at 540.349 kb on + strand, within GFF496at 540.349 kb on + strand, within GFF496at 540.349 kb on + strand, within GFF496at 540.350 kb on - strand, within GFF496at 540.431 kb on - strand, within GFF496at 540.526 kb on + strand, within GFF496at 540.527 kb on - strand, within GFF496at 540.607 kb on + strand, within GFF496at 540.607 kb on + strand, within GFF496at 540.607 kb on + strand, within GFF496at 540.607 kb on + strand, within GFF496at 540.607 kb on + strand, within GFF496at 540.607 kb on + strand, within GFF496at 540.607 kb on + strand, within GFF496at 540.608 kb on - strand, within GFF496at 540.608 kb on - strand, within GFF496at 540.608 kb on - strand, within GFF496at 540.608 kb on - strand, within GFF496at 540.608 kb on - strand, within GFF496at 540.608 kb on - strand, within GFF496at 540.608 kb on - strand, within GFF496at 540.682 kb on + strand, within GFF496at 540.683 kb on - strand, within GFF496at 540.683 kb on - strand, within GFF496at 540.683 kb on - strand, within GFF496at 540.683 kb on - strand, within GFF496at 540.793 kb on + strand, within GFF496at 540.793 kb on + strand, within GFF496at 540.794 kb on - strand, within GFF496at 540.794 kb on - strand, within GFF496at 540.794 kb on - strand, within GFF496at 540.794 kb on - strand, within GFF496at 540.913 kb on + strand, within GFF496at 540.914 kb on - strand, within GFF496at 541.170 kb on + strandat 541.170 kb on + strandat 541.170 kb on + strandat 541.170 kb on + strandat 541.170 kb on + strandat 541.170 kb on + strandat 541.170 kb on + strandat 541.170 kb on + strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.171 kb on - strandat 541.210 kb on - strandat 541.212 kb on + strandat 541.212 kb on + strandat 541.212 kb on + strandat 541.212 kb on + strandat 541.213 kb on - strandat 541.213 kb on - strandat 541.213 kb on - strandat 541.263 kb on + strand, within GFF497at 541.264 kb on - strand, within GFF497at 541.264 kb on - strand, within GFF497at 541.264 kb on - strand, within GFF497at 541.422 kb on + strand, within GFF497at 541.423 kb on - strand, within GFF497at 541.492 kb on - strand, within GFF497at 541.492 kb on - strand, within GFF497at 541.492 kb on - strand, within GFF497at 541.492 kb on - strand, within GFF497at 541.597 kb on - strand, within GFF497at 541.677 kb on + strand, within GFF497at 541.677 kb on + strand, within GFF497at 541.677 kb on + strand, within GFF497at 541.677 kb on + strand, within GFF497at 541.677 kb on + strand, within GFF497at 541.677 kb on - strand, within GFF497at 541.678 kb on - strand, within GFF497at 541.678 kb on - strand, within GFF497at 541.678 kb on - strand, within GFF497at 541.678 kb on - strand, within GFF497at 541.797 kb on + strand, within GFF497at 541.797 kb on + strand, within GFF497at 541.872 kb on + strand, within GFF497at 541.872 kb on + strand, within GFF497at 541.872 kb on + strand, within GFF497at 541.872 kb on + strand, within GFF497at 541.872 kb on + strand, within GFF497at 541.873 kb on - strand, within GFF497at 541.873 kb on - strand, within GFF497at 541.991 kb on - strandat 541.991 kb on - strandat 541.991 kb on - strandat 541.991 kb on - strandat 541.991 kb on - strandat 542.302 kb on + strand, within GFF498at 542.302 kb on + strand, within GFF498at 542.302 kb on + strand, within GFF498at 542.302 kb on + strand, within GFF498at 542.303 kb on - strand, within GFF498at 542.303 kb on - strand, within GFF498at 542.980 kb on + strand, within GFF498at 542.980 kb on + strand, within GFF498at 542.980 kb on + strand, within GFF498at 542.980 kb on + strand, within GFF498at 542.980 kb on + strand, within GFF498at 542.981 kb on - strand, within GFF498at 542.981 kb on - strand, within GFF498at 542.981 kb on - strand, within GFF498at 542.981 kb on - strand, within GFF498

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
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540,206 + +2.9
540,207 - -1.7
540,207 - -1.5
540,283 + -0.9
540,283 + +0.3
540,283 + -0.9
540,284 - -0.6
540,284 - -0.8
540,349 + GFF496 0.11 +0.3
540,349 + GFF496 0.11 +0.9
540,349 + GFF496 0.11 +0.2
540,349 + GFF496 0.11 -0.3
540,350 - GFF496 0.12 +0.6
540,431 - GFF496 0.20 +0.1
540,526 + GFF496 0.31 -0.1
540,527 - GFF496 0.31 -1.3
540,607 + GFF496 0.40 -1.7
540,607 + GFF496 0.40 -0.6
540,607 + GFF496 0.40 -1.5
540,607 + GFF496 0.40 -0.4
540,607 + GFF496 0.40 -1.5
540,607 + GFF496 0.40 -1.6
540,607 + GFF496 0.40 +0.3
540,608 - GFF496 0.40 +0.4
540,608 - GFF496 0.40 -1.0
540,608 - GFF496 0.40 +0.1
540,608 - GFF496 0.40 -2.5
540,608 - GFF496 0.40 -0.8
540,608 - GFF496 0.40 -0.6
540,608 - GFF496 0.40 +0.5
540,682 + GFF496 0.48 -0.0
540,683 - GFF496 0.48 -1.5
540,683 - GFF496 0.48 -1.7
540,683 - GFF496 0.48 -0.6
540,683 - GFF496 0.48 +0.1
540,793 + GFF496 0.60 -0.1
540,793 + GFF496 0.60 -1.7
540,794 - GFF496 0.60 -1.6
540,794 - GFF496 0.60 -0.6
540,794 - GFF496 0.60 -1.1
540,794 - GFF496 0.60 -1.0
540,913 + GFF496 0.73 -0.4
540,914 - GFF496 0.73 +1.3
541,170 + +0.7
541,170 + -0.3
541,170 + -0.5
541,170 + -1.3
541,170 + -0.1
541,170 + -0.8
541,170 + -0.8
541,170 + -1.1
541,171 - -0.2
541,171 - -0.8
541,171 - -1.8
541,171 - +0.7
541,171 - -1.0
541,171 - +1.3
541,171 - -0.0
541,171 - -0.4
541,171 - +0.3
541,171 - -3.1
541,171 - -0.1
541,171 - -0.8
541,171 - -1.5
541,171 - -0.1
541,171 - -1.6
541,210 - -0.5
541,212 + -0.9
541,212 + -0.3
541,212 + -0.8
541,212 + -1.1
541,213 - +0.3
541,213 - -0.7
541,213 - -0.9
541,263 + GFF497 0.13 +1.3
541,264 - GFF497 0.13 -0.3
541,264 - GFF497 0.13 -1.9
541,264 - GFF497 0.13 -1.0
541,422 + GFF497 0.32 -1.3
541,423 - GFF497 0.32 -1.6
541,492 - GFF497 0.40 -0.5
541,492 - GFF497 0.40 -0.6
541,492 - GFF497 0.40 -0.3
541,492 - GFF497 0.40 -1.5
541,597 - GFF497 0.53 -2.1
541,677 + GFF497 0.62 -1.8
541,677 + GFF497 0.62 -3.5
541,677 + GFF497 0.62 -1.8
541,677 + GFF497 0.62 -3.2
541,677 + GFF497 0.62 -1.1
541,677 - GFF497 0.62 -0.2
541,678 - GFF497 0.62 -1.0
541,678 - GFF497 0.62 -1.5
541,678 - GFF497 0.62 -0.0
541,678 - GFF497 0.62 -3.1
541,797 + GFF497 0.76 -1.2
541,797 + GFF497 0.76 -0.3
541,872 + GFF497 0.85 -0.9
541,872 + GFF497 0.85 -1.3
541,872 + GFF497 0.85 -0.0
541,872 + GFF497 0.85 -0.4
541,872 + GFF497 0.85 -0.5
541,873 - GFF497 0.85 -3.0
541,873 - GFF497 0.85 -0.0
541,991 - -2.1
541,991 - -1.0
541,991 - -1.9
541,991 - -1.8
541,991 - -1.0
542,302 + GFF498 0.14 +0.1
542,302 + GFF498 0.14 +0.3
542,302 + GFF498 0.14 +1.5
542,302 + GFF498 0.14 +0.5
542,303 - GFF498 0.14 -1.3
542,303 - GFF498 0.14 -0.3
542,980 + GFF498 0.68 -2.4
542,980 + GFF498 0.68 -1.2
542,980 + GFF498 0.68 -0.1
542,980 + GFF498 0.68 -0.3
542,980 + GFF498 0.68 -0.0
542,981 - GFF498 0.68 +0.6
542,981 - GFF498 0.68 +0.0
542,981 - GFF498 0.68 -0.9
542,981 - GFF498 0.68 +0.1

Or see this region's nucleotide sequence