Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4923 and GFF4924 are separated by 40 nucleotides GFF4924 and GFF4925 overlap by 8 nucleotides GFF4925 and GFF4926 are separated by 69 nucleotides GFF4926 and GFF4927 are separated by 73 nucleotides
GFF4923 - Substrate-specific component BioY of biotin ECF transporter, at 15,201 to 15,791
GFF4923
GFF4924 - Transmembrane component BioN of energizing module of biotin ECF transporter, at 15,832 to 16,425
GFF4924
GFF4925 - ATPase component BioM of energizing module of biotin ECF transporter, at 16,418 to 17,137
GFF4925
GFF4926 - Probable carboxyvinyl-carboxyphosphonate phosphorylmutase (EC 2.7.8.23), at 17,207 to 18,049
GFF4926
GFF4927 - Citrate-proton symporter, at 18,123 to 19,418
GFF4927
Position (kb)
16
17
18 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 15.748 kb on + strand at 15.748 kb on + strand at 15.748 kb on + strand at 15.748 kb on + strand at 15.748 kb on + strand at 15.748 kb on + strand at 15.748 kb on + strand at 15.748 kb on + strand at 15.748 kb on + strand at 15.748 kb on + strand at 15.748 kb on + strand at 15.749 kb on - strand at 15.749 kb on - strand at 15.749 kb on - strand at 15.749 kb on - strand at 15.749 kb on - strand at 15.749 kb on - strand at 15.749 kb on - strand at 15.749 kb on - strand at 15.749 kb on - strand at 15.749 kb on - strand at 15.749 kb on - strand at 15.749 kb on - strand at 15.947 kb on + strand, within GFF4924 at 15.947 kb on + strand, within GFF4924 at 15.947 kb on + strand, within GFF4924 at 15.977 kb on + strand, within GFF4924 at 15.978 kb on - strand, within GFF4924 at 16.412 kb on + strand at 16.413 kb on - strand at 16.413 kb on - strand at 16.419 kb on + strand at 16.470 kb on + strand at 16.548 kb on + strand, within GFF4925 at 16.548 kb on + strand, within GFF4925 at 16.549 kb on - strand, within GFF4925 at 17.179 kb on - strand at 17.415 kb on + strand, within GFF4926 at 17.415 kb on + strand, within GFF4926 at 17.416 kb on - strand, within GFF4926 at 17.416 kb on - strand, within GFF4926 at 17.514 kb on + strand, within GFF4926 at 17.514 kb on + strand, within GFF4926 at 17.577 kb on + strand, within GFF4926 at 17.577 kb on + strand, within GFF4926 at 17.577 kb on + strand, within GFF4926 at 17.578 kb on - strand, within GFF4926 at 17.578 kb on - strand, within GFF4926 at 17.624 kb on + strand, within GFF4926 at 17.648 kb on + strand, within GFF4926 at 17.649 kb on - strand, within GFF4926 at 17.649 kb on - strand, within GFF4926 at 17.649 kb on - strand, within GFF4926 at 17.649 kb on - strand, within GFF4926 at 17.871 kb on + strand, within GFF4926 at 17.871 kb on + strand, within GFF4926 at 18.087 kb on + strand at 18.088 kb on - strand at 18.088 kb on - strand at 18.124 kb on + strand at 18.125 kb on - strand at 18.137 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 15,748 + +2.4 15,748 + -0.0 15,748 + -0.7 15,748 + -0.8 15,748 + +1.4 15,748 + +0.9 15,748 + -0.7 15,748 + -0.6 15,748 + -0.2 15,748 + -0.2 15,748 + +2.6 15,749 - -0.2 15,749 - -1.2 15,749 - +0.4 15,749 - +0.1 15,749 - -0.6 15,749 - +0.0 15,749 - -0.7 15,749 - +0.1 15,749 - +0.0 15,749 - -0.4 15,749 - -0.5 15,749 - -0.8 15,947 + GFF4924 0.19 -0.9 15,947 + GFF4924 0.19 -0.2 15,947 + GFF4924 0.19 +0.2 15,977 + GFF4924 0.24 -0.1 15,978 - GFF4924 0.25 -0.7 16,412 + +1.4 16,413 - +0.2 16,413 - -0.8 16,419 + +0.6 16,470 + -1.4 16,548 + GFF4925 0.18 -0.3 16,548 + GFF4925 0.18 -1.5 16,549 - GFF4925 0.18 -2.5 17,179 - -1.1 17,415 + GFF4926 0.25 -1.4 17,415 + GFF4926 0.25 -0.2 17,416 - GFF4926 0.25 +0.2 17,416 - GFF4926 0.25 -0.0 17,514 + GFF4926 0.36 +0.2 17,514 + GFF4926 0.36 -1.4 17,577 + GFF4926 0.44 -0.3 17,577 + GFF4926 0.44 -3.2 17,577 + GFF4926 0.44 -0.8 17,578 - GFF4926 0.44 -0.7 17,578 - GFF4926 0.44 +1.2 17,624 + GFF4926 0.49 +2.8 17,648 + GFF4926 0.52 -1.2 17,649 - GFF4926 0.52 +0.2 17,649 - GFF4926 0.52 -0.7 17,649 - GFF4926 0.52 +0.9 17,649 - GFF4926 0.52 -1.8 17,871 + GFF4926 0.79 +2.1 17,871 + GFF4926 0.79 -0.2 18,087 + -0.6 18,088 - -0.9 18,088 - +0.1 18,124 + -0.2 18,125 - -0.2 18,137 - -0.2
Or see this region's nucleotide sequence