Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4849 and GFF4850 overlap by 4 nucleotides GFF4850 and GFF4851 overlap by 4 nucleotides GFF4851 and GFF4852 are separated by 217 nucleotides GFF4852 and GFF4853 are separated by 25 nucleotides
GFF4849 - Conjugative transfer protein TrbB, at 218,804 to 219,841
GFF4849
GFF4850 - CopG domain-containing protein, at 219,838 to 220,302
GFF4850
GFF4851 - Coupling protein VirD4, ATPase required for T-DNA transfer, at 220,299 to 222,317
GFF4851
GFF4852 - Conjugative transfer protein TrbK, at 222,535 to 222,765
GFF4852
GFF4853 - Transcriptional regulator, LysR family, at 222,791 to 223,705
GFF4853
Position (kb)
220
221
222
223 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 219.453 kb on + strand, within GFF4849 at 219.841 kb on + strand at 219.841 kb on + strand at 219.842 kb on - strand at 219.842 kb on - strand at 219.842 kb on - strand at 220.302 kb on + strand at 220.302 kb on + strand at 220.302 kb on + strand at 220.302 kb on + strand at 220.302 kb on + strand at 220.302 kb on + strand at 220.302 kb on + strand at 220.303 kb on - strand at 220.303 kb on - strand at 220.303 kb on - strand at 220.303 kb on - strand at 220.303 kb on - strand at 220.303 kb on - strand at 220.447 kb on + strand at 220.447 kb on + strand at 220.447 kb on + strand at 220.447 kb on + strand at 220.447 kb on + strand at 220.447 kb on + strand at 220.447 kb on + strand at 220.448 kb on - strand at 220.448 kb on - strand at 220.448 kb on - strand at 220.448 kb on - strand at 220.681 kb on + strand, within GFF4851 at 220.681 kb on + strand, within GFF4851 at 220.682 kb on - strand, within GFF4851 at 220.682 kb on - strand, within GFF4851 at 220.684 kb on + strand, within GFF4851 at 220.685 kb on - strand, within GFF4851 at 220.758 kb on + strand, within GFF4851 at 220.758 kb on + strand, within GFF4851 at 220.759 kb on - strand, within GFF4851 at 220.759 kb on - strand, within GFF4851 at 220.759 kb on - strand, within GFF4851 at 220.847 kb on - strand, within GFF4851 at 220.975 kb on + strand, within GFF4851 at 220.976 kb on - strand, within GFF4851 at 220.976 kb on - strand, within GFF4851 at 221.103 kb on + strand, within GFF4851 at 221.103 kb on + strand, within GFF4851 at 221.103 kb on + strand, within GFF4851 at 221.103 kb on + strand, within GFF4851 at 221.104 kb on - strand, within GFF4851 at 221.104 kb on - strand, within GFF4851 at 221.234 kb on - strand, within GFF4851 at 221.234 kb on - strand, within GFF4851 at 221.534 kb on - strand, within GFF4851 at 221.534 kb on - strand, within GFF4851 at 221.534 kb on - strand, within GFF4851 at 221.534 kb on - strand, within GFF4851 at 221.534 kb on - strand, within GFF4851 at 221.692 kb on + strand, within GFF4851 at 221.906 kb on - strand, within GFF4851 at 221.906 kb on - strand, within GFF4851 at 221.906 kb on - strand, within GFF4851 at 221.906 kb on - strand, within GFF4851 at 221.906 kb on - strand, within GFF4851 at 221.999 kb on - strand, within GFF4851 at 221.999 kb on - strand, within GFF4851 at 222.054 kb on + strand, within GFF4851 at 222.054 kb on + strand, within GFF4851 at 222.054 kb on + strand, within GFF4851 at 222.054 kb on + strand, within GFF4851 at 222.055 kb on - strand, within GFF4851 at 222.055 kb on - strand, within GFF4851 at 222.055 kb on - strand, within GFF4851 at 222.055 kb on - strand, within GFF4851 at 222.055 kb on - strand, within GFF4851 at 222.055 kb on - strand, within GFF4851 at 222.055 kb on - strand, within GFF4851 at 222.055 kb on - strand, within GFF4851 at 222.055 kb on - strand, within GFF4851 at 222.055 kb on - strand, within GFF4851 at 222.055 kb on - strand, within GFF4851 at 222.108 kb on - strand, within GFF4851 at 222.108 kb on - strand, within GFF4851 at 222.118 kb on + strand at 222.163 kb on + strand at 222.163 kb on + strand at 222.164 kb on - strand at 222.164 kb on - strand at 222.164 kb on - strand at 222.207 kb on + strand at 222.207 kb on + strand at 222.207 kb on + strand at 222.207 kb on + strand at 222.207 kb on + strand at 222.208 kb on - strand at 222.208 kb on - strand at 222.208 kb on - strand at 222.208 kb on - strand at 222.214 kb on + strand at 222.252 kb on + strand at 222.252 kb on + strand at 222.333 kb on + strand at 222.399 kb on - strand at 222.452 kb on - strand at 222.452 kb on - strand at 222.492 kb on + strand at 222.492 kb on + strand at 222.493 kb on - strand at 222.493 kb on - strand at 222.493 kb on - strand at 222.493 kb on - strand at 222.531 kb on + strand at 222.531 kb on + strand at 222.532 kb on - strand at 222.532 kb on - strand at 222.536 kb on + strand at 222.536 kb on + strand at 222.536 kb on + strand at 222.537 kb on - strand at 222.537 kb on - strand at 222.537 kb on - strand at 222.537 kb on - strand at 222.897 kb on + strand, within GFF4853 at 222.897 kb on + strand, within GFF4853 at 222.897 kb on + strand, within GFF4853 at 222.897 kb on + strand, within GFF4853 at 222.897 kb on + strand, within GFF4853 at 222.897 kb on + strand, within GFF4853 at 222.897 kb on + strand, within GFF4853 at 222.897 kb on + strand, within GFF4853 at 222.897 kb on + strand, within GFF4853 at 222.898 kb on - strand, within GFF4853 at 222.898 kb on - strand, within GFF4853 at 222.898 kb on - strand, within GFF4853 at 222.898 kb on - strand, within GFF4853 at 222.898 kb on - strand, within GFF4853 at 222.898 kb on - strand, within GFF4853 at 222.898 kb on - strand, within GFF4853 at 222.898 kb on - strand, within GFF4853 at 222.898 kb on - strand, within GFF4853 at 223.010 kb on + strand, within GFF4853 at 223.010 kb on + strand, within GFF4853 at 223.010 kb on + strand, within GFF4853 at 223.011 kb on - strand, within GFF4853 at 223.011 kb on - strand, within GFF4853 at 223.011 kb on - strand, within GFF4853 at 223.011 kb on - strand, within GFF4853 at 223.011 kb on - strand, within GFF4853 at 223.029 kb on + strand, within GFF4853 at 223.029 kb on + strand, within GFF4853 at 223.030 kb on - strand, within GFF4853 at 223.030 kb on - strand, within GFF4853 at 223.030 kb on - strand, within GFF4853 at 223.030 kb on - strand, within GFF4853 at 223.030 kb on - strand, within GFF4853 at 223.030 kb on - strand, within GFF4853 at 223.030 kb on - strand, within GFF4853 at 223.030 kb on - strand, within GFF4853 at 223.069 kb on - strand, within GFF4853 at 223.069 kb on - strand, within GFF4853 at 223.112 kb on + strand, within GFF4853 at 223.284 kb on + strand, within GFF4853 at 223.284 kb on + strand, within GFF4853 at 223.284 kb on + strand, within GFF4853 at 223.284 kb on + strand, within GFF4853 at 223.284 kb on + strand, within GFF4853 at 223.284 kb on + strand, within GFF4853 at 223.284 kb on + strand, within GFF4853 at 223.284 kb on + strand, within GFF4853 at 223.284 kb on + strand, within GFF4853 at 223.285 kb on - strand, within GFF4853 at 223.285 kb on - strand, within GFF4853 at 223.285 kb on - strand, within GFF4853 at 223.285 kb on - strand, within GFF4853
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 219,453 + GFF4849 0.63 -0.5 219,841 + -0.3 219,841 + +0.3 219,842 - -0.2 219,842 - -0.6 219,842 - +1.0 220,302 + -0.3 220,302 + +0.5 220,302 + +0.7 220,302 + +0.7 220,302 + +0.3 220,302 + -0.6 220,302 + +0.0 220,303 - +1.1 220,303 - +0.1 220,303 - +0.1 220,303 - +0.2 220,303 - +0.5 220,303 - +0.2 220,447 + -0.5 220,447 + -0.2 220,447 + +0.7 220,447 + -2.0 220,447 + +0.1 220,447 + -0.7 220,447 + -0.6 220,448 - +0.0 220,448 - +1.4 220,448 - -0.4 220,448 - +0.2 220,681 + GFF4851 0.19 -1.2 220,681 + GFF4851 0.19 +1.2 220,682 - GFF4851 0.19 -2.0 220,682 - GFF4851 0.19 -0.5 220,684 + GFF4851 0.19 +0.2 220,685 - GFF4851 0.19 +0.2 220,758 + GFF4851 0.23 -0.3 220,758 + GFF4851 0.23 +0.4 220,759 - GFF4851 0.23 +0.4 220,759 - GFF4851 0.23 +0.2 220,759 - GFF4851 0.23 -0.1 220,847 - GFF4851 0.27 +0.4 220,975 + GFF4851 0.33 -0.5 220,976 - GFF4851 0.34 -0.9 220,976 - GFF4851 0.34 +0.2 221,103 + GFF4851 0.40 -1.2 221,103 + GFF4851 0.40 -0.0 221,103 + GFF4851 0.40 +0.8 221,103 + GFF4851 0.40 -2.4 221,104 - GFF4851 0.40 +1.3 221,104 - GFF4851 0.40 +0.5 221,234 - GFF4851 0.46 +0.3 221,234 - GFF4851 0.46 -0.9 221,534 - GFF4851 0.61 -0.9 221,534 - GFF4851 0.61 -0.6 221,534 - GFF4851 0.61 +0.4 221,534 - GFF4851 0.61 -0.8 221,534 - GFF4851 0.61 +0.1 221,692 + GFF4851 0.69 -1.2 221,906 - GFF4851 0.80 +0.1 221,906 - GFF4851 0.80 -0.7 221,906 - GFF4851 0.80 +0.7 221,906 - GFF4851 0.80 -0.9 221,906 - GFF4851 0.80 +0.0 221,999 - GFF4851 0.84 -0.5 221,999 - GFF4851 0.84 +0.5 222,054 + GFF4851 0.87 +2.0 222,054 + GFF4851 0.87 -0.9 222,054 + GFF4851 0.87 +0.8 222,054 + GFF4851 0.87 +0.1 222,055 - GFF4851 0.87 +0.6 222,055 - GFF4851 0.87 -0.2 222,055 - GFF4851 0.87 -0.7 222,055 - GFF4851 0.87 -1.2 222,055 - GFF4851 0.87 -0.3 222,055 - GFF4851 0.87 -0.4 222,055 - GFF4851 0.87 -1.7 222,055 - GFF4851 0.87 +0.1 222,055 - GFF4851 0.87 +0.9 222,055 - GFF4851 0.87 -0.0 222,055 - GFF4851 0.87 +0.4 222,108 - GFF4851 0.90 -1.4 222,108 - GFF4851 0.90 -0.5 222,118 + +0.1 222,163 + -0.2 222,163 + -0.3 222,164 - -0.2 222,164 - -1.0 222,164 - -1.0 222,207 + -0.3 222,207 + -0.0 222,207 + -0.8 222,207 + +1.1 222,207 + +0.0 222,208 - +0.4 222,208 - -0.0 222,208 - +0.7 222,208 - +1.3 222,214 + -1.6 222,252 + -0.4 222,252 + -0.2 222,333 + +1.4 222,399 - -0.9 222,452 - +1.7 222,452 - -1.0 222,492 + -0.6 222,492 + -0.4 222,493 - +0.9 222,493 - -0.7 222,493 - -0.3 222,493 - -0.5 222,531 + +0.2 222,531 + -1.6 222,532 - -0.2 222,532 - +0.4 222,536 + -1.4 222,536 + +1.7 222,536 + -0.7 222,537 - +0.1 222,537 - +0.8 222,537 - +0.5 222,537 - -0.0 222,897 + GFF4853 0.12 -0.4 222,897 + GFF4853 0.12 +0.7 222,897 + GFF4853 0.12 +0.3 222,897 + GFF4853 0.12 -0.5 222,897 + GFF4853 0.12 -0.0 222,897 + GFF4853 0.12 -0.1 222,897 + GFF4853 0.12 -0.7 222,897 + GFF4853 0.12 -0.1 222,897 + GFF4853 0.12 +0.2 222,898 - GFF4853 0.12 +1.6 222,898 - GFF4853 0.12 +0.0 222,898 - GFF4853 0.12 -0.7 222,898 - GFF4853 0.12 +1.2 222,898 - GFF4853 0.12 +0.4 222,898 - GFF4853 0.12 +1.7 222,898 - GFF4853 0.12 -0.0 222,898 - GFF4853 0.12 -0.7 222,898 - GFF4853 0.12 -0.1 223,010 + GFF4853 0.24 -0.4 223,010 + GFF4853 0.24 -1.3 223,010 + GFF4853 0.24 -3.7 223,011 - GFF4853 0.24 -0.8 223,011 - GFF4853 0.24 -0.6 223,011 - GFF4853 0.24 -0.7 223,011 - GFF4853 0.24 +0.1 223,011 - GFF4853 0.24 +0.2 223,029 + GFF4853 0.26 -1.3 223,029 + GFF4853 0.26 +0.5 223,030 - GFF4853 0.26 +0.1 223,030 - GFF4853 0.26 -0.7 223,030 - GFF4853 0.26 +1.6 223,030 - GFF4853 0.26 -0.3 223,030 - GFF4853 0.26 -0.7 223,030 - GFF4853 0.26 +0.4 223,030 - GFF4853 0.26 -1.7 223,030 - GFF4853 0.26 +0.4 223,069 - GFF4853 0.30 -0.6 223,069 - GFF4853 0.30 -0.2 223,112 + GFF4853 0.35 +0.4 223,284 + GFF4853 0.54 -1.6 223,284 + GFF4853 0.54 -0.7 223,284 + GFF4853 0.54 -0.5 223,284 + GFF4853 0.54 +0.2 223,284 + GFF4853 0.54 -0.0 223,284 + GFF4853 0.54 -0.6 223,284 + GFF4853 0.54 +0.4 223,284 + GFF4853 0.54 +0.4 223,284 + GFF4853 0.54 -0.7 223,285 - GFF4853 0.54 -0.2 223,285 - GFF4853 0.54 -0.0 223,285 - GFF4853 0.54 +0.4 223,285 - GFF4853 0.54 -1.0
Or see this region's nucleotide sequence