Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4735 and GFF4736 overlap by 4 nucleotides GFF4736 and GFF4737 are separated by 13 nucleotides GFF4737 and GFF4738 are separated by 330 nucleotides
GFF4735 - Nitrate ABC transporter, ATP-binding protein, at 101,244 to 102,053
GFF4735
GFF4736 - Nitrate ABC transporter, permease protein, at 102,050 to 102,970
GFF4736
GFF4737 - Nitrate ABC transporter, substrate-binding protein, at 102,984 to 104,228
GFF4737
GFF4738 - Response regulator NasT, at 104,559 to 105,875
GFF4738
Position (kb)
102
103
104
105 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 102.034 kb on + strand at 102.034 kb on + strand at 102.034 kb on + strand at 102.035 kb on - strand at 102.282 kb on + strand, within GFF4736 at 102.282 kb on + strand, within GFF4736 at 102.282 kb on + strand, within GFF4736 at 102.282 kb on + strand, within GFF4736 at 102.282 kb on + strand, within GFF4736 at 102.282 kb on + strand, within GFF4736 at 102.282 kb on + strand, within GFF4736 at 102.283 kb on - strand, within GFF4736 at 102.360 kb on + strand, within GFF4736 at 102.361 kb on - strand, within GFF4736 at 102.361 kb on - strand, within GFF4736 at 102.675 kb on + strand, within GFF4736 at 102.675 kb on + strand, within GFF4736 at 102.676 kb on - strand, within GFF4736 at 102.676 kb on - strand, within GFF4736 at 102.676 kb on - strand, within GFF4736 at 102.676 kb on - strand, within GFF4736 at 102.676 kb on - strand, within GFF4736 at 103.012 kb on + strand at 103.012 kb on + strand at 103.117 kb on + strand, within GFF4737 at 103.117 kb on + strand, within GFF4737 at 103.117 kb on + strand, within GFF4737 at 103.117 kb on + strand, within GFF4737 at 103.117 kb on + strand, within GFF4737 at 103.118 kb on - strand, within GFF4737 at 103.118 kb on - strand, within GFF4737 at 103.118 kb on - strand, within GFF4737 at 103.156 kb on + strand, within GFF4737 at 103.156 kb on + strand, within GFF4737 at 103.157 kb on - strand, within GFF4737 at 103.225 kb on + strand, within GFF4737 at 103.225 kb on + strand, within GFF4737 at 103.225 kb on + strand, within GFF4737 at 103.226 kb on - strand, within GFF4737 at 103.252 kb on + strand, within GFF4737 at 103.252 kb on + strand, within GFF4737 at 103.252 kb on + strand, within GFF4737 at 103.253 kb on - strand, within GFF4737 at 103.253 kb on - strand, within GFF4737 at 103.303 kb on + strand, within GFF4737 at 103.304 kb on - strand, within GFF4737 at 103.351 kb on + strand, within GFF4737 at 103.352 kb on - strand, within GFF4737 at 103.352 kb on - strand, within GFF4737 at 103.352 kb on - strand, within GFF4737 at 103.352 kb on - strand, within GFF4737 at 103.655 kb on - strand, within GFF4737 at 103.702 kb on + strand, within GFF4737 at 103.702 kb on + strand, within GFF4737 at 103.750 kb on + strand, within GFF4737 at 103.750 kb on + strand, within GFF4737 at 103.750 kb on + strand, within GFF4737 at 103.750 kb on + strand, within GFF4737 at 103.751 kb on - strand, within GFF4737 at 103.751 kb on - strand, within GFF4737 at 103.913 kb on - strand, within GFF4737 at 103.913 kb on - strand, within GFF4737 at 103.924 kb on + strand, within GFF4737 at 103.924 kb on + strand, within GFF4737 at 103.925 kb on - strand, within GFF4737 at 104.017 kb on + strand, within GFF4737 at 104.018 kb on - strand, within GFF4737 at 104.018 kb on - strand, within GFF4737 at 104.399 kb on + strand at 104.399 kb on + strand at 104.399 kb on + strand at 104.399 kb on + strand at 104.399 kb on + strand at 104.400 kb on - strand at 104.400 kb on - strand at 104.400 kb on - strand at 104.593 kb on + strand at 104.593 kb on + strand at 104.593 kb on + strand at 104.593 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 102,034 + -1.9 102,034 + -1.9 102,034 + -0.7 102,035 - -0.7 102,282 + GFF4736 0.25 +0.0 102,282 + GFF4736 0.25 +0.1 102,282 + GFF4736 0.25 -0.5 102,282 + GFF4736 0.25 -0.9 102,282 + GFF4736 0.25 -0.3 102,282 + GFF4736 0.25 +1.4 102,282 + GFF4736 0.25 +1.0 102,283 - GFF4736 0.25 +1.6 102,360 + GFF4736 0.34 +0.4 102,361 - GFF4736 0.34 +0.1 102,361 - GFF4736 0.34 -0.2 102,675 + GFF4736 0.68 -0.8 102,675 + GFF4736 0.68 +0.9 102,676 - GFF4736 0.68 -0.7 102,676 - GFF4736 0.68 -0.1 102,676 - GFF4736 0.68 -0.6 102,676 - GFF4736 0.68 +0.6 102,676 - GFF4736 0.68 +0.5 103,012 + -0.2 103,012 + +0.6 103,117 + GFF4737 0.11 -0.1 103,117 + GFF4737 0.11 -1.8 103,117 + GFF4737 0.11 -1.2 103,117 + GFF4737 0.11 +0.6 103,117 + GFF4737 0.11 +0.5 103,118 - GFF4737 0.11 +0.7 103,118 - GFF4737 0.11 -0.3 103,118 - GFF4737 0.11 +0.2 103,156 + GFF4737 0.14 +0.6 103,156 + GFF4737 0.14 +1.8 103,157 - GFF4737 0.14 -0.1 103,225 + GFF4737 0.19 +0.3 103,225 + GFF4737 0.19 -0.1 103,225 + GFF4737 0.19 -1.4 103,226 - GFF4737 0.19 -0.1 103,252 + GFF4737 0.22 -0.1 103,252 + GFF4737 0.22 -0.1 103,252 + GFF4737 0.22 -0.1 103,253 - GFF4737 0.22 +0.1 103,253 - GFF4737 0.22 -0.0 103,303 + GFF4737 0.26 +0.3 103,304 - GFF4737 0.26 +1.0 103,351 + GFF4737 0.29 -0.0 103,352 - GFF4737 0.30 -0.8 103,352 - GFF4737 0.30 +0.2 103,352 - GFF4737 0.30 -1.1 103,352 - GFF4737 0.30 -0.0 103,655 - GFF4737 0.54 +0.4 103,702 + GFF4737 0.58 -0.6 103,702 + GFF4737 0.58 +0.2 103,750 + GFF4737 0.62 +1.4 103,750 + GFF4737 0.62 -2.0 103,750 + GFF4737 0.62 -0.6 103,750 + GFF4737 0.62 +1.2 103,751 - GFF4737 0.62 -1.5 103,751 - GFF4737 0.62 +0.3 103,913 - GFF4737 0.75 -0.5 103,913 - GFF4737 0.75 +0.7 103,924 + GFF4737 0.76 +0.1 103,924 + GFF4737 0.76 +0.1 103,925 - GFF4737 0.76 -0.3 104,017 + GFF4737 0.83 -0.2 104,018 - GFF4737 0.83 -1.8 104,018 - GFF4737 0.83 +0.4 104,399 + +0.3 104,399 + -0.5 104,399 + +0.4 104,399 + -1.1 104,399 + -1.4 104,400 - -1.4 104,400 - -0.7 104,400 - +0.1 104,593 + +0.3 104,593 + -1.1 104,593 + -0.9 104,593 + +0.7
Or see this region's nucleotide sequence