Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4713 and GFF4714 are separated by 11 nucleotides GFF4714 and GFF4715 overlap by 1 nucleotides GFF4715 and GFF4716 are separated by 147 nucleotides GFF4716 and GFF4717 are separated by 10 nucleotides
GFF4713 - Uncharacterized protein YhiN, at 75,263 to 76,468
GFF4713
GFF4714 - putative lipoprotein, at 76,480 to 77,271
GFF4714
GFF4715 - Fatty acid hydroxylase family (carotene hydroxylase/sterol desaturase), at 77,271 to 78,401
GFF4715
GFF4716 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 78,549 to 79,382
GFF4716
GFF4717 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 79,393 to 80,190
GFF4717
Position (kb) 77
78
79 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 76.517 kb on + strand at 76.517 kb on + strand at 76.517 kb on + strand at 76.517 kb on + strand at 76.517 kb on + strand at 76.517 kb on + strand at 76.517 kb on + strand at 76.517 kb on + strand at 76.518 kb on - strand at 76.518 kb on - strand at 76.518 kb on - strand at 76.535 kb on + strand at 76.535 kb on + strand at 76.535 kb on + strand at 76.536 kb on - strand at 76.536 kb on - strand at 76.536 kb on - strand at 76.653 kb on - strand, within GFF4714 at 76.653 kb on - strand, within GFF4714 at 76.653 kb on - strand, within GFF4714 at 76.736 kb on + strand, within GFF4714 at 76.736 kb on + strand, within GFF4714 at 76.737 kb on - strand, within GFF4714 at 76.737 kb on - strand, within GFF4714 at 76.737 kb on - strand, within GFF4714 at 76.737 kb on - strand, within GFF4714 at 76.737 kb on - strand, within GFF4714 at 76.826 kb on + strand, within GFF4714 at 76.826 kb on + strand, within GFF4714 at 76.827 kb on - strand, within GFF4714 at 76.827 kb on - strand, within GFF4714 at 76.827 kb on - strand, within GFF4714 at 76.827 kb on - strand, within GFF4714 at 76.827 kb on - strand, within GFF4714 at 76.997 kb on + strand, within GFF4714 at 76.997 kb on + strand, within GFF4714 at 76.997 kb on + strand, within GFF4714 at 76.998 kb on - strand, within GFF4714 at 76.998 kb on - strand, within GFF4714 at 76.998 kb on - strand, within GFF4714 at 76.998 kb on - strand, within GFF4714 at 76.998 kb on - strand, within GFF4714 at 77.212 kb on + strand at 77.212 kb on + strand at 77.213 kb on - strand at 77.326 kb on + strand at 77.327 kb on - strand at 77.327 kb on - strand at 77.327 kb on - strand at 77.344 kb on + strand at 77.344 kb on + strand at 77.344 kb on + strand at 77.345 kb on - strand at 77.345 kb on - strand at 77.345 kb on - strand at 77.345 kb on - strand at 77.345 kb on - strand at 77.392 kb on + strand, within GFF4715 at 77.392 kb on + strand, within GFF4715 at 77.392 kb on + strand, within GFF4715 at 77.392 kb on + strand, within GFF4715 at 77.392 kb on + strand, within GFF4715 at 77.393 kb on - strand, within GFF4715 at 77.393 kb on - strand, within GFF4715 at 77.393 kb on - strand, within GFF4715 at 77.393 kb on - strand, within GFF4715 at 77.393 kb on - strand, within GFF4715 at 77.393 kb on - strand, within GFF4715 at 77.393 kb on - strand, within GFF4715 at 77.393 kb on - strand, within GFF4715 at 77.393 kb on - strand, within GFF4715 at 77.393 kb on - strand, within GFF4715 at 77.393 kb on - strand, within GFF4715 at 77.407 kb on + strand, within GFF4715 at 77.407 kb on + strand, within GFF4715 at 77.407 kb on + strand, within GFF4715 at 77.407 kb on + strand, within GFF4715 at 77.407 kb on + strand, within GFF4715 at 77.408 kb on - strand, within GFF4715 at 77.419 kb on + strand, within GFF4715 at 77.419 kb on + strand, within GFF4715 at 77.419 kb on + strand, within GFF4715 at 77.419 kb on + strand, within GFF4715 at 77.420 kb on - strand, within GFF4715 at 77.420 kb on - strand, within GFF4715 at 77.420 kb on - strand, within GFF4715 at 77.420 kb on - strand, within GFF4715 at 77.420 kb on - strand, within GFF4715 at 77.420 kb on - strand, within GFF4715 at 77.488 kb on + strand, within GFF4715 at 77.489 kb on - strand, within GFF4715 at 77.489 kb on - strand, within GFF4715 at 77.489 kb on - strand, within GFF4715 at 77.560 kb on + strand, within GFF4715 at 77.560 kb on + strand, within GFF4715 at 77.560 kb on + strand, within GFF4715 at 77.560 kb on + strand, within GFF4715 at 77.560 kb on + strand, within GFF4715 at 77.560 kb on + strand, within GFF4715 at 77.560 kb on + strand, within GFF4715 at 77.560 kb on + strand, within GFF4715 at 77.560 kb on + strand, within GFF4715 at 77.561 kb on - strand, within GFF4715 at 77.596 kb on + strand, within GFF4715 at 77.596 kb on + strand, within GFF4715 at 77.596 kb on + strand, within GFF4715 at 77.596 kb on + strand, within GFF4715 at 77.596 kb on + strand, within GFF4715 at 77.596 kb on + strand, within GFF4715 at 77.596 kb on + strand, within GFF4715 at 77.596 kb on + strand, within GFF4715 at 77.597 kb on - strand, within GFF4715 at 77.597 kb on - strand, within GFF4715 at 77.597 kb on - strand, within GFF4715 at 77.597 kb on - strand, within GFF4715 at 77.597 kb on - strand, within GFF4715 at 77.597 kb on - strand, within GFF4715 at 77.597 kb on - strand, within GFF4715 at 77.597 kb on - strand, within GFF4715 at 77.597 kb on - strand, within GFF4715 at 77.597 kb on - strand, within GFF4715 at 77.597 kb on - strand, within GFF4715 at 77.728 kb on + strand, within GFF4715 at 77.728 kb on + strand, within GFF4715 at 77.729 kb on - strand, within GFF4715 at 77.729 kb on - strand, within GFF4715 at 77.746 kb on + strand, within GFF4715 at 77.746 kb on + strand, within GFF4715 at 77.747 kb on - strand, within GFF4715 at 77.747 kb on - strand, within GFF4715 at 77.747 kb on - strand, within GFF4715 at 77.747 kb on - strand, within GFF4715 at 78.043 kb on + strand, within GFF4715 at 78.043 kb on + strand, within GFF4715 at 78.043 kb on + strand, within GFF4715 at 78.043 kb on + strand, within GFF4715 at 78.043 kb on + strand, within GFF4715 at 78.208 kb on + strand, within GFF4715 at 78.208 kb on + strand, within GFF4715 at 78.208 kb on + strand, within GFF4715 at 78.208 kb on + strand, within GFF4715 at 78.209 kb on - strand, within GFF4715 at 78.209 kb on - strand, within GFF4715 at 78.209 kb on - strand, within GFF4715 at 78.209 kb on - strand, within GFF4715 at 78.209 kb on - strand, within GFF4715 at 78.209 kb on - strand, within GFF4715 at 78.209 kb on - strand, within GFF4715 at 78.209 kb on - strand, within GFF4715 at 78.229 kb on + strand, within GFF4715 at 78.229 kb on + strand, within GFF4715 at 78.229 kb on + strand, within GFF4715 at 78.230 kb on - strand, within GFF4715 at 78.230 kb on - strand, within GFF4715 at 78.230 kb on - strand, within GFF4715 at 78.230 kb on - strand, within GFF4715 at 78.230 kb on - strand, within GFF4715 at 78.230 kb on - strand, within GFF4715 at 78.259 kb on + strand, within GFF4715 at 78.260 kb on - strand, within GFF4715 at 78.260 kb on - strand, within GFF4715 at 78.260 kb on - strand, within GFF4715 at 78.260 kb on - strand, within GFF4715 at 78.260 kb on - strand, within GFF4715 at 78.447 kb on - strand at 78.510 kb on + strand at 78.511 kb on - strand at 78.511 kb on - strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.518 kb on + strand at 78.519 kb on - strand at 78.519 kb on - strand at 78.519 kb on - strand at 78.519 kb on - strand at 78.519 kb on - strand at 78.519 kb on - strand at 78.519 kb on - strand at 78.519 kb on - strand at 78.519 kb on - strand at 78.519 kb on - strand at 78.519 kb on - strand at 78.519 kb on - strand at 78.519 kb on - strand at 78.520 kb on + strand at 78.521 kb on - strand at 78.531 kb on + strand at 78.531 kb on + strand at 78.531 kb on + strand at 78.531 kb on + strand at 78.532 kb on - strand at 78.532 kb on - strand at 78.702 kb on + strand, within GFF4716 at 78.795 kb on + strand, within GFF4716 at 78.795 kb on + strand, within GFF4716 at 78.795 kb on + strand, within GFF4716 at 78.795 kb on + strand, within GFF4716 at 78.796 kb on - strand, within GFF4716 at 78.796 kb on - strand, within GFF4716 at 78.927 kb on + strand, within GFF4716 at 78.927 kb on + strand, within GFF4716 at 78.927 kb on + strand, within GFF4716 at 78.927 kb on + strand, within GFF4716 at 78.927 kb on + strand, within GFF4716 at 78.927 kb on + strand, within GFF4716 at 78.927 kb on + strand, within GFF4716 at 78.927 kb on + strand, within GFF4716 at 78.927 kb on + strand, within GFF4716 at 78.927 kb on + strand, within GFF4716 at 78.928 kb on - strand, within GFF4716 at 78.928 kb on - strand, within GFF4716 at 78.928 kb on - strand, within GFF4716 at 78.928 kb on - strand, within GFF4716 at 78.928 kb on - strand, within GFF4716 at 78.928 kb on - strand, within GFF4716 at 78.928 kb on - strand, within GFF4716 at 78.928 kb on - strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.185 kb on + strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.186 kb on - strand, within GFF4716 at 79.203 kb on + strand, within GFF4716
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 76,517 + +0.7 76,517 + -0.6 76,517 + -0.7 76,517 + +1.1 76,517 + +1.4 76,517 + +0.0 76,517 + -0.4 76,517 + -0.2 76,518 - -0.3 76,518 - +0.7 76,518 - -2.2 76,535 + -0.1 76,535 + +0.7 76,535 + -0.7 76,536 - -0.3 76,536 - -0.2 76,536 - -0.6 76,653 - GFF4714 0.22 -0.4 76,653 - GFF4714 0.22 -0.4 76,653 - GFF4714 0.22 -0.5 76,736 + GFF4714 0.32 -0.1 76,736 + GFF4714 0.32 +0.2 76,737 - GFF4714 0.32 -1.9 76,737 - GFF4714 0.32 -0.2 76,737 - GFF4714 0.32 +0.7 76,737 - GFF4714 0.32 +0.5 76,737 - GFF4714 0.32 -0.0 76,826 + GFF4714 0.44 -0.4 76,826 + GFF4714 0.44 -0.3 76,827 - GFF4714 0.44 +0.3 76,827 - GFF4714 0.44 -0.4 76,827 - GFF4714 0.44 -0.5 76,827 - GFF4714 0.44 -0.0 76,827 - GFF4714 0.44 -0.7 76,997 + GFF4714 0.65 -0.1 76,997 + GFF4714 0.65 -0.1 76,997 + GFF4714 0.65 -0.2 76,998 - GFF4714 0.65 -3.6 76,998 - GFF4714 0.65 -1.4 76,998 - GFF4714 0.65 -2.7 76,998 - GFF4714 0.65 -1.5 76,998 - GFF4714 0.65 +0.6 77,212 + -0.8 77,212 + -1.6 77,213 - +0.2 77,326 + -0.6 77,327 - +0.4 77,327 - -0.6 77,327 - -0.6 77,344 + +0.4 77,344 + -0.8 77,344 + -2.0 77,345 - +0.6 77,345 - +4.0 77,345 - +0.2 77,345 - +0.4 77,345 - -0.2 77,392 + GFF4715 0.11 -1.3 77,392 + GFF4715 0.11 +0.3 77,392 + GFF4715 0.11 +0.9 77,392 + GFF4715 0.11 -1.0 77,392 + GFF4715 0.11 +0.7 77,393 - GFF4715 0.11 -0.0 77,393 - GFF4715 0.11 -0.1 77,393 - GFF4715 0.11 -0.6 77,393 - GFF4715 0.11 -0.7 77,393 - GFF4715 0.11 -0.4 77,393 - GFF4715 0.11 -1.0 77,393 - GFF4715 0.11 -0.5 77,393 - GFF4715 0.11 -0.4 77,393 - GFF4715 0.11 +0.4 77,393 - GFF4715 0.11 +0.4 77,393 - GFF4715 0.11 +1.0 77,407 + GFF4715 0.12 -0.1 77,407 + GFF4715 0.12 -0.5 77,407 + GFF4715 0.12 -0.0 77,407 + GFF4715 0.12 -0.7 77,407 + GFF4715 0.12 -0.4 77,408 - GFF4715 0.12 +0.7 77,419 + GFF4715 0.13 +1.7 77,419 + GFF4715 0.13 -1.3 77,419 + GFF4715 0.13 -1.3 77,419 + GFF4715 0.13 -0.2 77,420 - GFF4715 0.13 +1.0 77,420 - GFF4715 0.13 +0.1 77,420 - GFF4715 0.13 +0.2 77,420 - GFF4715 0.13 -1.1 77,420 - GFF4715 0.13 -0.9 77,420 - GFF4715 0.13 -0.7 77,488 + GFF4715 0.19 -1.3 77,489 - GFF4715 0.19 -0.4 77,489 - GFF4715 0.19 -0.3 77,489 - GFF4715 0.19 -1.6 77,560 + GFF4715 0.26 +0.4 77,560 + GFF4715 0.26 +1.2 77,560 + GFF4715 0.26 -0.8 77,560 + GFF4715 0.26 +0.5 77,560 + GFF4715 0.26 -0.9 77,560 + GFF4715 0.26 -0.8 77,560 + GFF4715 0.26 +0.3 77,560 + GFF4715 0.26 +0.4 77,560 + GFF4715 0.26 -1.1 77,561 - GFF4715 0.26 +1.0 77,596 + GFF4715 0.29 -0.4 77,596 + GFF4715 0.29 -0.5 77,596 + GFF4715 0.29 -0.6 77,596 + GFF4715 0.29 -0.9 77,596 + GFF4715 0.29 -1.6 77,596 + GFF4715 0.29 +0.5 77,596 + GFF4715 0.29 -0.1 77,596 + GFF4715 0.29 -0.6 77,597 - GFF4715 0.29 +1.1 77,597 - GFF4715 0.29 -1.0 77,597 - GFF4715 0.29 -0.2 77,597 - GFF4715 0.29 -1.0 77,597 - GFF4715 0.29 -1.9 77,597 - GFF4715 0.29 -1.1 77,597 - GFF4715 0.29 -1.4 77,597 - GFF4715 0.29 +0.0 77,597 - GFF4715 0.29 +0.1 77,597 - GFF4715 0.29 +0.0 77,597 - GFF4715 0.29 -0.0 77,728 + GFF4715 0.40 -0.1 77,728 + GFF4715 0.40 -0.3 77,729 - GFF4715 0.40 -0.4 77,729 - GFF4715 0.40 +0.1 77,746 + GFF4715 0.42 -0.4 77,746 + GFF4715 0.42 -0.6 77,747 - GFF4715 0.42 -0.2 77,747 - GFF4715 0.42 -0.8 77,747 - GFF4715 0.42 -1.3 77,747 - GFF4715 0.42 +0.6 78,043 + GFF4715 0.68 -0.3 78,043 + GFF4715 0.68 +0.6 78,043 + GFF4715 0.68 -0.3 78,043 + GFF4715 0.68 +0.0 78,043 + GFF4715 0.68 +0.4 78,208 + GFF4715 0.83 -0.2 78,208 + GFF4715 0.83 -0.2 78,208 + GFF4715 0.83 -0.6 78,208 + GFF4715 0.83 +1.0 78,209 - GFF4715 0.83 -1.0 78,209 - GFF4715 0.83 -0.5 78,209 - GFF4715 0.83 -0.3 78,209 - GFF4715 0.83 -0.9 78,209 - GFF4715 0.83 -0.5 78,209 - GFF4715 0.83 -1.0 78,209 - GFF4715 0.83 -1.2 78,209 - GFF4715 0.83 +0.1 78,229 + GFF4715 0.85 -1.2 78,229 + GFF4715 0.85 -0.3 78,229 + GFF4715 0.85 +0.8 78,230 - GFF4715 0.85 -0.5 78,230 - GFF4715 0.85 -0.8 78,230 - GFF4715 0.85 +0.2 78,230 - GFF4715 0.85 -1.0 78,230 - GFF4715 0.85 +1.0 78,230 - GFF4715 0.85 -2.1 78,259 + GFF4715 0.87 -0.6 78,260 - GFF4715 0.87 -1.0 78,260 - GFF4715 0.87 +0.7 78,260 - GFF4715 0.87 -0.0 78,260 - GFF4715 0.87 +0.2 78,260 - GFF4715 0.87 -2.6 78,447 - +0.5 78,510 + -2.2 78,511 - -0.2 78,511 - -0.9 78,518 + +0.6 78,518 + +1.0 78,518 + -0.1 78,518 + -0.7 78,518 + -0.5 78,518 + +1.3 78,518 + -0.4 78,518 + +0.0 78,518 + -0.5 78,518 + -0.5 78,518 + -0.3 78,518 + +0.7 78,518 + +0.3 78,518 + +0.0 78,518 + -0.5 78,518 + -0.4 78,519 - -0.6 78,519 - +0.0 78,519 - -0.2 78,519 - -1.4 78,519 - -2.2 78,519 - -0.5 78,519 - -0.9 78,519 - +0.1 78,519 - +0.8 78,519 - -0.0 78,519 - +0.2 78,519 - +0.1 78,519 - +0.1 78,520 + +1.4 78,521 - +0.3 78,531 + -0.4 78,531 + -0.6 78,531 + +0.3 78,531 + +0.1 78,532 - -0.2 78,532 - +1.4 78,702 + GFF4716 0.18 +0.2 78,795 + GFF4716 0.29 +0.0 78,795 + GFF4716 0.29 +1.4 78,795 + GFF4716 0.29 -0.2 78,795 + GFF4716 0.29 -1.3 78,796 - GFF4716 0.30 -0.2 78,796 - GFF4716 0.30 +1.2 78,927 + GFF4716 0.45 +0.4 78,927 + GFF4716 0.45 -0.6 78,927 + GFF4716 0.45 +0.1 78,927 + GFF4716 0.45 +0.0 78,927 + GFF4716 0.45 -0.2 78,927 + GFF4716 0.45 +0.2 78,927 + GFF4716 0.45 +0.4 78,927 + GFF4716 0.45 -1.9 78,927 + GFF4716 0.45 +0.1 78,927 + GFF4716 0.45 -0.2 78,928 - GFF4716 0.45 +0.2 78,928 - GFF4716 0.45 +2.0 78,928 - GFF4716 0.45 -0.3 78,928 - GFF4716 0.45 +0.5 78,928 - GFF4716 0.45 -0.6 78,928 - GFF4716 0.45 -0.3 78,928 - GFF4716 0.45 -0.5 78,928 - GFF4716 0.45 -0.4 79,185 + GFF4716 0.76 -1.4 79,185 + GFF4716 0.76 +0.2 79,185 + GFF4716 0.76 +0.9 79,185 + GFF4716 0.76 -0.6 79,185 + GFF4716 0.76 +0.2 79,185 + GFF4716 0.76 -0.7 79,185 + GFF4716 0.76 +0.3 79,185 + GFF4716 0.76 -0.9 79,185 + GFF4716 0.76 +0.3 79,185 + GFF4716 0.76 +0.3 79,185 + GFF4716 0.76 -0.2 79,185 + GFF4716 0.76 +0.1 79,185 + GFF4716 0.76 +0.5 79,185 + GFF4716 0.76 +0.1 79,185 + GFF4716 0.76 -0.3 79,185 + GFF4716 0.76 -0.5 79,186 - GFF4716 0.76 -0.4 79,186 - GFF4716 0.76 -0.8 79,186 - GFF4716 0.76 -0.1 79,186 - GFF4716 0.76 -0.8 79,186 - GFF4716 0.76 +0.4 79,186 - GFF4716 0.76 +0.5 79,186 - GFF4716 0.76 -0.8 79,186 - GFF4716 0.76 -0.5 79,186 - GFF4716 0.76 +0.1 79,186 - GFF4716 0.76 -0.3 79,186 - GFF4716 0.76 +1.4 79,186 - GFF4716 0.76 -0.9 79,186 - GFF4716 0.76 +0.9 79,186 - GFF4716 0.76 -2.3 79,186 - GFF4716 0.76 +0.2 79,186 - GFF4716 0.76 -0.5 79,186 - GFF4716 0.76 -0.2 79,186 - GFF4716 0.76 +1.1 79,203 + GFF4716 0.78 -1.0
Or see this region's nucleotide sequence