Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4590 and GFF4591 are separated by 27 nucleotides GFF4591 and GFF4592 are separated by 1 nucleotides GFF4592 and GFF4593 are separated by 98 nucleotides
GFF4590 - Glutamate/aspartate ABC transporter, ATP-binding protein GltL (TC 3.A.1.3.4), at 231,172 to 231,921
GFF4590
GFF4591 - Glutamate/aspartate ABC transporter, permease protein GltK (TC 3.A.1.3.4), at 231,949 to 232,617
GFF4591
GFF4592 - Glutamate/aspartate ABC transporter, permease protein GltJ (TC 3.A.1.3.4), at 232,619 to 233,377
GFF4592
GFF4593 - Glutamate/aspartate ABC transporter, substrate-binding protein GltI (TC 3.A.1.3.4), at 233,476 to 234,372
GFF4593
Position (kb) 232
233
234 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 231.878 kb on + strand at 231.879 kb on - strand at 231.889 kb on + strand at 231.889 kb on + strand at 231.889 kb on + strand at 231.889 kb on + strand at 231.889 kb on + strand at 231.890 kb on - strand at 232.004 kb on + strand at 232.004 kb on + strand at 232.005 kb on - strand at 232.019 kb on + strand, within GFF4591 at 232.020 kb on - strand, within GFF4591 at 232.040 kb on + strand, within GFF4591 at 232.041 kb on - strand, within GFF4591 at 232.041 kb on - strand, within GFF4591 at 232.041 kb on - strand, within GFF4591 at 232.041 kb on - strand, within GFF4591 at 232.041 kb on - strand, within GFF4591 at 232.115 kb on + strand, within GFF4591 at 232.116 kb on - strand, within GFF4591 at 232.116 kb on - strand, within GFF4591 at 232.116 kb on - strand, within GFF4591 at 232.116 kb on - strand, within GFF4591 at 232.220 kb on + strand, within GFF4591 at 232.220 kb on + strand, within GFF4591 at 232.220 kb on + strand, within GFF4591 at 232.220 kb on + strand, within GFF4591 at 232.220 kb on + strand, within GFF4591 at 232.220 kb on + strand, within GFF4591 at 232.220 kb on + strand, within GFF4591 at 232.220 kb on + strand, within GFF4591 at 232.220 kb on + strand, within GFF4591 at 232.220 kb on + strand, within GFF4591 at 232.221 kb on - strand, within GFF4591 at 232.221 kb on - strand, within GFF4591 at 232.221 kb on - strand, within GFF4591 at 232.301 kb on + strand, within GFF4591 at 232.301 kb on + strand, within GFF4591 at 232.301 kb on + strand, within GFF4591 at 232.302 kb on - strand, within GFF4591 at 232.302 kb on - strand, within GFF4591 at 232.302 kb on - strand, within GFF4591 at 232.323 kb on - strand, within GFF4591 at 232.436 kb on + strand, within GFF4591 at 232.437 kb on - strand, within GFF4591 at 232.437 kb on - strand, within GFF4591 at 232.550 kb on + strand, within GFF4591 at 232.550 kb on + strand, within GFF4591 at 232.551 kb on - strand at 232.551 kb on - strand at 232.551 kb on - strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.592 kb on + strand at 232.592 kb on + strand at 232.592 kb on + strand at 232.592 kb on + strand at 232.593 kb on - strand at 232.593 kb on - strand at 232.719 kb on + strand, within GFF4592 at 232.719 kb on + strand, within GFF4592 at 232.719 kb on + strand, within GFF4592 at 232.719 kb on + strand, within GFF4592 at 232.719 kb on + strand, within GFF4592 at 232.719 kb on + strand, within GFF4592 at 232.719 kb on + strand, within GFF4592 at 232.719 kb on + strand, within GFF4592 at 232.719 kb on + strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.740 kb on + strand, within GFF4592 at 232.740 kb on + strand, within GFF4592 at 232.741 kb on - strand, within GFF4592 at 232.741 kb on - strand, within GFF4592 at 232.782 kb on + strand, within GFF4592 at 232.782 kb on + strand, within GFF4592 at 232.908 kb on + strand, within GFF4592 at 232.908 kb on + strand, within GFF4592 at 232.908 kb on + strand, within GFF4592 at 232.908 kb on + strand, within GFF4592 at 232.909 kb on - strand, within GFF4592 at 232.909 kb on - strand, within GFF4592 at 232.911 kb on + strand, within GFF4592 at 232.911 kb on + strand, within GFF4592 at 232.911 kb on + strand, within GFF4592 at 232.911 kb on + strand, within GFF4592 at 232.911 kb on + strand, within GFF4592 at 232.911 kb on + strand, within GFF4592 at 232.911 kb on + strand, within GFF4592 at 232.911 kb on + strand, within GFF4592 at 232.912 kb on - strand, within GFF4592 at 232.912 kb on - strand, within GFF4592 at 232.912 kb on - strand, within GFF4592 at 232.948 kb on - strand, within GFF4592 at 233.091 kb on + strand, within GFF4592 at 233.091 kb on + strand, within GFF4592 at 233.092 kb on - strand, within GFF4592 at 233.092 kb on - strand, within GFF4592 at 233.092 kb on - strand, within GFF4592 at 233.092 kb on - strand, within GFF4592 at 233.194 kb on + strand, within GFF4592 at 233.195 kb on - strand, within GFF4592 at 233.195 kb on - strand, within GFF4592 at 233.195 kb on - strand, within GFF4592 at 233.195 kb on - strand, within GFF4592 at 233.195 kb on - strand, within GFF4592 at 233.286 kb on + strand, within GFF4592 at 233.286 kb on + strand, within GFF4592 at 233.286 kb on + strand, within GFF4592 at 233.287 kb on - strand, within GFF4592 at 233.287 kb on - strand, within GFF4592 at 233.287 kb on - strand, within GFF4592 at 233.287 kb on - strand, within GFF4592 at 233.287 kb on - strand, within GFF4592 at 233.412 kb on + strand at 233.412 kb on + strand at 233.412 kb on + strand at 233.412 kb on + strand at 233.412 kb on + strand at 233.412 kb on + strand at 233.412 kb on + strand at 233.413 kb on - strand at 233.413 kb on - strand at 233.413 kb on - strand at 233.413 kb on - strand at 233.413 kb on - strand at 233.413 kb on - strand at 233.477 kb on + strand at 233.477 kb on + strand at 233.478 kb on - strand at 233.478 kb on - strand at 233.478 kb on - strand at 233.489 kb on + strand at 233.489 kb on + strand at 233.490 kb on - strand at 233.535 kb on + strand at 233.535 kb on + strand at 233.535 kb on + strand at 233.535 kb on + strand at 233.536 kb on - strand at 233.603 kb on + strand, within GFF4593 at 233.604 kb on - strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.008 kb on + strand, within GFF4593 at 234.009 kb on - strand, within GFF4593 at 234.009 kb on - strand, within GFF4593 at 234.009 kb on - strand, within GFF4593 at 234.009 kb on - strand, within GFF4593 at 234.009 kb on - strand, within GFF4593 at 234.009 kb on - strand, within GFF4593 at 234.009 kb on - strand, within GFF4593 at 234.009 kb on - strand, within GFF4593 at 234.009 kb on - strand, within GFF4593 at 234.009 kb on - strand, within GFF4593 at 234.009 kb on - strand, within GFF4593 at 234.017 kb on + strand, within GFF4593 at 234.017 kb on + strand, within GFF4593 at 234.017 kb on + strand, within GFF4593 at 234.018 kb on - strand, within GFF4593 at 234.018 kb on - strand, within GFF4593 at 234.018 kb on - strand, within GFF4593 at 234.131 kb on + strand, within GFF4593 at 234.132 kb on - strand, within GFF4593 at 234.132 kb on - strand, within GFF4593 at 234.132 kb on - strand, within GFF4593 at 234.132 kb on - strand, within GFF4593 at 234.212 kb on + strand, within GFF4593 at 234.212 kb on + strand, within GFF4593 at 234.212 kb on + strand, within GFF4593 at 234.212 kb on + strand, within GFF4593 at 234.212 kb on + strand, within GFF4593 at 234.212 kb on + strand, within GFF4593 at 234.213 kb on - strand, within GFF4593 at 234.213 kb on - strand, within GFF4593 at 234.213 kb on - strand, within GFF4593 at 234.213 kb on - strand, within GFF4593 at 234.213 kb on - strand, within GFF4593 at 234.213 kb on - strand, within GFF4593 at 234.213 kb on - strand, within GFF4593 at 234.213 kb on - strand, within GFF4593 at 234.213 kb on - strand, within GFF4593 at 234.233 kb on + strand, within GFF4593 at 234.233 kb on + strand, within GFF4593 at 234.233 kb on + strand, within GFF4593 at 234.234 kb on - strand, within GFF4593 at 234.234 kb on - strand, within GFF4593 at 234.234 kb on - strand, within GFF4593 at 234.234 kb on - strand, within GFF4593
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 231,878 + -0.1 231,879 - -1.4 231,889 + -1.3 231,889 + -1.0 231,889 + -1.2 231,889 + -0.3 231,889 + -0.7 231,890 - -0.5 232,004 + +0.4 232,004 + -0.0 232,005 - -0.8 232,019 + GFF4591 0.10 -1.5 232,020 - GFF4591 0.11 +0.3 232,040 + GFF4591 0.14 -3.0 232,041 - GFF4591 0.14 +1.3 232,041 - GFF4591 0.14 -0.3 232,041 - GFF4591 0.14 -1.4 232,041 - GFF4591 0.14 -0.6 232,041 - GFF4591 0.14 -2.3 232,115 + GFF4591 0.25 +0.4 232,116 - GFF4591 0.25 +0.7 232,116 - GFF4591 0.25 -0.4 232,116 - GFF4591 0.25 -2.2 232,116 - GFF4591 0.25 +2.3 232,220 + GFF4591 0.41 -0.1 232,220 + GFF4591 0.41 -0.4 232,220 + GFF4591 0.41 -1.4 232,220 + GFF4591 0.41 -1.4 232,220 + GFF4591 0.41 +0.2 232,220 + GFF4591 0.41 -0.8 232,220 + GFF4591 0.41 -0.3 232,220 + GFF4591 0.41 -0.5 232,220 + GFF4591 0.41 -1.0 232,220 + GFF4591 0.41 +0.0 232,221 - GFF4591 0.41 -0.7 232,221 - GFF4591 0.41 -0.4 232,221 - GFF4591 0.41 -0.0 232,301 + GFF4591 0.53 -0.8 232,301 + GFF4591 0.53 -0.9 232,301 + GFF4591 0.53 +0.3 232,302 - GFF4591 0.53 -0.6 232,302 - GFF4591 0.53 +0.5 232,302 - GFF4591 0.53 -1.4 232,323 - GFF4591 0.56 +1.2 232,436 + GFF4591 0.73 -0.3 232,437 - GFF4591 0.73 +0.2 232,437 - GFF4591 0.73 -1.4 232,550 + GFF4591 0.90 -1.1 232,550 + GFF4591 0.90 -0.1 232,551 - -0.3 232,551 - -0.2 232,551 - -0.8 232,568 + -1.2 232,568 + -0.3 232,568 + +0.2 232,568 + -1.8 232,568 + -0.5 232,568 + -0.4 232,568 + +0.2 232,568 + -1.0 232,568 + -0.8 232,568 + -0.5 232,568 + -0.2 232,568 + -1.5 232,568 + +0.4 232,568 + -0.7 232,568 + -0.5 232,568 + -1.0 232,568 + +0.3 232,568 + -0.5 232,568 + -2.5 232,568 + -0.0 232,568 + +0.0 232,569 - -0.8 232,569 - -0.9 232,569 - +0.3 232,569 - -1.8 232,569 - -1.8 232,569 - +1.0 232,569 - -1.3 232,569 - +0.4 232,569 - +0.9 232,569 - -0.6 232,569 - -0.5 232,569 - -0.3 232,569 - +0.4 232,569 - -0.1 232,569 - +1.4 232,592 + -0.4 232,592 + -0.5 232,592 + -1.3 232,592 + -0.1 232,593 - -0.5 232,593 - -2.6 232,719 + GFF4592 0.13 -2.8 232,719 + GFF4592 0.13 -0.2 232,719 + GFF4592 0.13 -1.3 232,719 + GFF4592 0.13 -2.1 232,719 + GFF4592 0.13 -0.4 232,719 + GFF4592 0.13 -0.0 232,719 + GFF4592 0.13 -0.7 232,719 + GFF4592 0.13 -1.1 232,719 + GFF4592 0.13 -0.3 232,720 - GFF4592 0.13 -0.6 232,720 - GFF4592 0.13 -0.1 232,720 - GFF4592 0.13 -2.0 232,720 - GFF4592 0.13 -0.1 232,720 - GFF4592 0.13 -0.3 232,720 - GFF4592 0.13 -0.6 232,720 - GFF4592 0.13 -2.2 232,720 - GFF4592 0.13 -0.8 232,720 - GFF4592 0.13 +0.9 232,720 - GFF4592 0.13 +0.2 232,740 + GFF4592 0.16 -1.3 232,740 + GFF4592 0.16 -0.1 232,741 - GFF4592 0.16 +0.5 232,741 - GFF4592 0.16 -0.6 232,782 + GFF4592 0.21 +0.5 232,782 + GFF4592 0.21 +0.7 232,908 + GFF4592 0.38 -0.7 232,908 + GFF4592 0.38 -2.6 232,908 + GFF4592 0.38 -1.1 232,908 + GFF4592 0.38 -0.5 232,909 - GFF4592 0.38 +1.4 232,909 - GFF4592 0.38 -0.6 232,911 + GFF4592 0.38 -1.3 232,911 + GFF4592 0.38 -1.5 232,911 + GFF4592 0.38 +0.6 232,911 + GFF4592 0.38 +0.1 232,911 + GFF4592 0.38 -1.1 232,911 + GFF4592 0.38 +3.3 232,911 + GFF4592 0.38 -2.0 232,911 + GFF4592 0.38 -0.9 232,912 - GFF4592 0.39 +0.5 232,912 - GFF4592 0.39 +0.0 232,912 - GFF4592 0.39 +0.5 232,948 - GFF4592 0.43 +0.1 233,091 + GFF4592 0.62 -0.8 233,091 + GFF4592 0.62 -0.9 233,092 - GFF4592 0.62 +0.4 233,092 - GFF4592 0.62 -1.6 233,092 - GFF4592 0.62 -0.3 233,092 - GFF4592 0.62 -0.3 233,194 + GFF4592 0.76 -0.7 233,195 - GFF4592 0.76 +0.4 233,195 - GFF4592 0.76 -0.7 233,195 - GFF4592 0.76 -0.5 233,195 - GFF4592 0.76 +0.5 233,195 - GFF4592 0.76 -0.7 233,286 + GFF4592 0.88 -0.7 233,286 + GFF4592 0.88 +0.2 233,286 + GFF4592 0.88 -0.1 233,287 - GFF4592 0.88 +0.1 233,287 - GFF4592 0.88 -0.7 233,287 - GFF4592 0.88 -0.3 233,287 - GFF4592 0.88 -0.1 233,287 - GFF4592 0.88 -1.3 233,412 + -1.3 233,412 + -1.6 233,412 + +0.3 233,412 + -0.1 233,412 + +0.8 233,412 + -0.5 233,412 + -1.0 233,413 - +0.9 233,413 - -0.3 233,413 - +1.7 233,413 - -0.1 233,413 - +0.1 233,413 - +0.0 233,477 + -0.4 233,477 + -0.2 233,478 - +0.1 233,478 - -0.4 233,478 - +0.0 233,489 + +0.7 233,489 + -0.7 233,490 - +0.2 233,535 + -0.9 233,535 + -1.8 233,535 + -0.2 233,535 + +0.4 233,536 - -0.8 233,603 + GFF4593 0.14 +0.8 233,604 - GFF4593 0.14 -0.1 234,008 + GFF4593 0.59 -0.3 234,008 + GFF4593 0.59 +0.0 234,008 + GFF4593 0.59 -0.8 234,008 + GFF4593 0.59 +1.1 234,008 + GFF4593 0.59 -0.0 234,008 + GFF4593 0.59 -0.8 234,008 + GFF4593 0.59 -0.4 234,008 + GFF4593 0.59 -2.2 234,008 + GFF4593 0.59 -0.3 234,008 + GFF4593 0.59 -1.1 234,008 + GFF4593 0.59 -1.2 234,008 + GFF4593 0.59 -0.3 234,008 + GFF4593 0.59 +1.3 234,009 - GFF4593 0.59 -0.5 234,009 - GFF4593 0.59 -3.3 234,009 - GFF4593 0.59 -0.8 234,009 - GFF4593 0.59 +0.8 234,009 - GFF4593 0.59 -0.1 234,009 - GFF4593 0.59 -3.2 234,009 - GFF4593 0.59 -2.3 234,009 - GFF4593 0.59 -0.0 234,009 - GFF4593 0.59 -0.3 234,009 - GFF4593 0.59 -0.9 234,009 - GFF4593 0.59 -1.1 234,017 + GFF4593 0.60 -0.6 234,017 + GFF4593 0.60 +0.2 234,017 + GFF4593 0.60 -0.9 234,018 - GFF4593 0.60 -0.3 234,018 - GFF4593 0.60 +0.0 234,018 - GFF4593 0.60 -0.3 234,131 + GFF4593 0.73 -0.1 234,132 - GFF4593 0.73 -0.6 234,132 - GFF4593 0.73 -1.0 234,132 - GFF4593 0.73 -1.4 234,132 - GFF4593 0.73 -0.6 234,212 + GFF4593 0.82 +0.3 234,212 + GFF4593 0.82 +0.2 234,212 + GFF4593 0.82 -1.7 234,212 + GFF4593 0.82 -0.5 234,212 + GFF4593 0.82 -3.0 234,212 + GFF4593 0.82 -0.8 234,213 - GFF4593 0.82 -0.6 234,213 - GFF4593 0.82 -0.4 234,213 - GFF4593 0.82 -0.2 234,213 - GFF4593 0.82 +0.4 234,213 - GFF4593 0.82 -0.6 234,213 - GFF4593 0.82 -0.3 234,213 - GFF4593 0.82 -1.8 234,213 - GFF4593 0.82 +0.3 234,213 - GFF4593 0.82 -1.1 234,233 + GFF4593 0.84 -0.3 234,233 + GFF4593 0.84 -0.5 234,233 + GFF4593 0.84 -1.1 234,234 - GFF4593 0.85 -0.3 234,234 - GFF4593 0.85 +0.0 234,234 - GFF4593 0.85 +0.4 234,234 - GFF4593 0.85 +0.7
Or see this region's nucleotide sequence