Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4555 and GFF4556 overlap by 4 nucleotides GFF4556 and GFF4557 overlap by 4 nucleotides GFF4557 and GFF4558 overlap by 4 nucleotides GFF4558 and GFF4559 are separated by 3 nucleotides GFF4559 and GFF4560 are separated by 97 nucleotides
GFF4555 - Putative transmembrane protein, at 192,074 to 192,829
GFF4555
GFF4556 - hypothetical protein, at 192,826 to 193,626
GFF4556
GFF4557 - Dihydroorotase (EC 3.5.2.3), at 193,623 to 194,654
GFF4557
GFF4558 - RNA polymerase ECF-type sigma factor, at 194,651 to 195,193
GFF4558
GFF4559 - Uncharacterized membrane protein Rmet_3714, at 195,197 to 195,406
GFF4559
GFF4560 - hypothetical protein, at 195,504 to 196,235
GFF4560
Position (kb)
193
194
195 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 192.651 kb on + strand, within GFF4555 at 192.652 kb on - strand, within GFF4555 at 192.652 kb on - strand, within GFF4555 at 192.652 kb on - strand, within GFF4555 at 192.695 kb on + strand, within GFF4555 at 192.695 kb on + strand, within GFF4555 at 192.696 kb on - strand, within GFF4555 at 192.696 kb on - strand, within GFF4555 at 192.696 kb on - strand, within GFF4555 at 192.696 kb on - strand, within GFF4555 at 193.232 kb on + strand, within GFF4556 at 193.232 kb on + strand, within GFF4556 at 193.232 kb on + strand, within GFF4556 at 193.232 kb on + strand, within GFF4556 at 193.232 kb on + strand, within GFF4556 at 193.232 kb on + strand, within GFF4556 at 193.232 kb on + strand, within GFF4556 at 193.233 kb on - strand, within GFF4556 at 193.233 kb on - strand, within GFF4556 at 193.233 kb on - strand, within GFF4556 at 193.233 kb on - strand, within GFF4556 at 193.233 kb on - strand, within GFF4556 at 193.233 kb on - strand, within GFF4556 at 193.233 kb on - strand, within GFF4556 at 193.233 kb on - strand, within GFF4556 at 193.233 kb on - strand, within GFF4556 at 193.505 kb on + strand, within GFF4556 at 193.505 kb on + strand, within GFF4556 at 193.505 kb on + strand, within GFF4556 at 193.506 kb on - strand, within GFF4556 at 193.506 kb on - strand, within GFF4556 at 193.506 kb on - strand, within GFF4556 at 193.506 kb on - strand, within GFF4556 at 193.506 kb on - strand, within GFF4556 at 193.506 kb on - strand, within GFF4556 at 193.532 kb on + strand, within GFF4556 at 193.533 kb on - strand, within GFF4556 at 193.756 kb on + strand, within GFF4557 at 193.756 kb on + strand, within GFF4557 at 193.756 kb on + strand, within GFF4557 at 193.756 kb on + strand, within GFF4557 at 193.757 kb on - strand, within GFF4557 at 193.757 kb on - strand, within GFF4557 at 193.858 kb on + strand, within GFF4557 at 194.005 kb on + strand, within GFF4557 at 194.005 kb on + strand, within GFF4557 at 194.005 kb on + strand, within GFF4557 at 194.006 kb on - strand, within GFF4557 at 194.006 kb on - strand, within GFF4557 at 194.006 kb on - strand, within GFF4557 at 194.006 kb on - strand, within GFF4557 at 194.008 kb on + strand, within GFF4557 at 194.008 kb on + strand, within GFF4557 at 194.009 kb on - strand, within GFF4557 at 194.009 kb on - strand, within GFF4557 at 194.009 kb on - strand, within GFF4557 at 194.009 kb on - strand, within GFF4557 at 194.050 kb on + strand, within GFF4557 at 194.050 kb on + strand, within GFF4557 at 194.050 kb on + strand, within GFF4557 at 194.050 kb on + strand, within GFF4557 at 194.051 kb on - strand, within GFF4557 at 194.051 kb on - strand, within GFF4557 at 194.051 kb on - strand, within GFF4557 at 194.107 kb on + strand, within GFF4557 at 194.107 kb on + strand, within GFF4557 at 194.107 kb on + strand, within GFF4557 at 194.108 kb on - strand, within GFF4557 at 194.296 kb on + strand, within GFF4557 at 194.296 kb on + strand, within GFF4557 at 194.296 kb on + strand, within GFF4557 at 194.296 kb on + strand, within GFF4557 at 194.296 kb on + strand, within GFF4557 at 194.297 kb on - strand, within GFF4557 at 194.297 kb on - strand, within GFF4557 at 194.297 kb on - strand, within GFF4557 at 194.297 kb on - strand, within GFF4557 at 194.297 kb on - strand, within GFF4557 at 194.297 kb on - strand, within GFF4557 at 194.297 kb on - strand, within GFF4557 at 194.297 kb on - strand, within GFF4557 at 194.353 kb on + strand, within GFF4557 at 194.688 kb on + strand at 194.689 kb on - strand at 194.814 kb on + strand, within GFF4558 at 194.814 kb on + strand, within GFF4558 at 194.815 kb on - strand, within GFF4558 at 194.815 kb on - strand, within GFF4558 at 194.815 kb on - strand, within GFF4558 at 194.815 kb on - strand, within GFF4558 at 194.815 kb on - strand, within GFF4558 at 195.030 kb on + strand, within GFF4558 at 195.088 kb on - strand, within GFF4558 at 195.399 kb on + strand at 195.399 kb on + strand at 195.399 kb on + strand at 195.399 kb on + strand at 195.399 kb on + strand at 195.399 kb on + strand at 195.399 kb on + strand at 195.399 kb on + strand at 195.399 kb on + strand at 195.399 kb on + strand at 195.399 kb on + strand at 195.399 kb on + strand at 195.399 kb on + strand at 195.399 kb on + strand at 195.399 kb on + strand at 195.400 kb on - strand at 195.400 kb on - strand at 195.400 kb on - strand at 195.400 kb on - strand at 195.505 kb on + strand at 195.505 kb on + strand at 195.505 kb on + strand at 195.505 kb on + strand at 195.505 kb on + strand at 195.506 kb on - strand at 195.506 kb on - strand at 195.506 kb on - strand at 195.506 kb on - strand at 195.506 kb on - strand at 195.506 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 192,651 + GFF4555 0.76 -0.4 192,652 - GFF4555 0.76 -0.5 192,652 - GFF4555 0.76 +0.1 192,652 - GFF4555 0.76 -0.1 192,695 + GFF4555 0.82 +0.2 192,695 + GFF4555 0.82 -0.2 192,696 - GFF4555 0.82 -0.2 192,696 - GFF4555 0.82 +0.1 192,696 - GFF4555 0.82 -0.8 192,696 - GFF4555 0.82 +0.2 193,232 + GFF4556 0.51 +0.8 193,232 + GFF4556 0.51 -0.4 193,232 + GFF4556 0.51 -0.7 193,232 + GFF4556 0.51 -0.1 193,232 + GFF4556 0.51 -0.9 193,232 + GFF4556 0.51 +0.3 193,232 + GFF4556 0.51 +1.2 193,233 - GFF4556 0.51 -0.5 193,233 - GFF4556 0.51 -0.0 193,233 - GFF4556 0.51 -0.6 193,233 - GFF4556 0.51 -0.2 193,233 - GFF4556 0.51 -0.2 193,233 - GFF4556 0.51 -1.6 193,233 - GFF4556 0.51 -0.6 193,233 - GFF4556 0.51 -0.5 193,233 - GFF4556 0.51 -0.4 193,505 + GFF4556 0.85 +0.5 193,505 + GFF4556 0.85 +1.2 193,505 + GFF4556 0.85 -1.3 193,506 - GFF4556 0.85 +3.3 193,506 - GFF4556 0.85 -1.6 193,506 - GFF4556 0.85 +0.6 193,506 - GFF4556 0.85 -0.8 193,506 - GFF4556 0.85 +0.9 193,506 - GFF4556 0.85 +0.5 193,532 + GFF4556 0.88 +1.3 193,533 - GFF4556 0.88 -0.3 193,756 + GFF4557 0.13 -2.1 193,756 + GFF4557 0.13 +1.7 193,756 + GFF4557 0.13 -0.6 193,756 + GFF4557 0.13 +0.7 193,757 - GFF4557 0.13 +1.3 193,757 - GFF4557 0.13 -0.1 193,858 + GFF4557 0.23 -0.3 194,005 + GFF4557 0.37 -0.8 194,005 + GFF4557 0.37 -0.1 194,005 + GFF4557 0.37 +0.2 194,006 - GFF4557 0.37 -1.4 194,006 - GFF4557 0.37 -1.2 194,006 - GFF4557 0.37 +0.7 194,006 - GFF4557 0.37 +2.3 194,008 + GFF4557 0.37 -0.8 194,008 + GFF4557 0.37 -1.0 194,009 - GFF4557 0.37 +0.7 194,009 - GFF4557 0.37 +1.2 194,009 - GFF4557 0.37 +0.2 194,009 - GFF4557 0.37 -0.8 194,050 + GFF4557 0.41 +1.7 194,050 + GFF4557 0.41 +1.2 194,050 + GFF4557 0.41 -0.3 194,050 + GFF4557 0.41 +0.2 194,051 - GFF4557 0.41 -2.1 194,051 - GFF4557 0.41 -0.3 194,051 - GFF4557 0.41 -2.7 194,107 + GFF4557 0.47 -0.3 194,107 + GFF4557 0.47 -1.3 194,107 + GFF4557 0.47 -0.6 194,108 - GFF4557 0.47 +2.7 194,296 + GFF4557 0.65 +1.7 194,296 + GFF4557 0.65 +0.2 194,296 + GFF4557 0.65 -2.6 194,296 + GFF4557 0.65 -1.3 194,296 + GFF4557 0.65 -0.8 194,297 - GFF4557 0.65 -0.3 194,297 - GFF4557 0.65 -1.3 194,297 - GFF4557 0.65 +0.2 194,297 - GFF4557 0.65 -0.6 194,297 - GFF4557 0.65 -1.6 194,297 - GFF4557 0.65 -0.7 194,297 - GFF4557 0.65 +2.3 194,297 - GFF4557 0.65 -0.3 194,353 + GFF4557 0.71 +1.0 194,688 + -0.2 194,689 - -1.4 194,814 + GFF4558 0.30 -1.8 194,814 + GFF4558 0.30 -1.7 194,815 - GFF4558 0.30 +1.3 194,815 - GFF4558 0.30 +0.4 194,815 - GFF4558 0.30 +0.9 194,815 - GFF4558 0.30 -0.0 194,815 - GFF4558 0.30 -0.2 195,030 + GFF4558 0.70 -0.6 195,088 - GFF4558 0.80 +0.6 195,399 + -0.5 195,399 + -0.2 195,399 + -1.1 195,399 + -2.3 195,399 + -0.3 195,399 + -0.1 195,399 + -1.4 195,399 + -0.2 195,399 + +0.7 195,399 + -0.6 195,399 + -1.6 195,399 + +1.2 195,399 + -2.3 195,399 + +0.5 195,399 + +0.0 195,400 - -0.5 195,400 - -0.4 195,400 - -0.9 195,400 - +0.1 195,505 + -0.7 195,505 + -0.9 195,505 + -1.7 195,505 + -0.5 195,505 + -1.5 195,506 - -1.7 195,506 - +0.4 195,506 - -0.4 195,506 - -1.0 195,506 - +1.2 195,506 - -0.1
Or see this region's nucleotide sequence