Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF446 and GFF447 overlap by 4 nucleotides GFF447 and GFF448 overlap by 4 nucleotides GFF448 and GFF449 overlap by 17 nucleotides
GFF446 - Iron(III) dicitrate transport system permease protein FecD (TC 3.A.1.14.1), at 484,125 to 485,150
GFF446
GFF447 - Vitamin B12 ABC transporter, substrate-binding protein BtuF, at 485,147 to 486,019
GFF447
GFF448 - Adenosylcobinamide kinase (EC 2.7.1.156) / Adenosylcobinamide-phosphate guanylyltransferase (EC 2.7.7.62), at 486,016 to 486,621
GFF448
GFF449 - Cobyrinic acid a,c-diamide synthetase (EC 6.3.5.11), at 486,605 to 488,026
GFF449
Position (kb)
486
487 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 485.367 kb on + strand, within GFF447 at 485.367 kb on + strand, within GFF447 at 485.367 kb on + strand, within GFF447 at 485.368 kb on - strand, within GFF447 at 485.368 kb on - strand, within GFF447 at 485.368 kb on - strand, within GFF447 at 485.368 kb on - strand, within GFF447 at 485.497 kb on - strand, within GFF447 at 485.497 kb on - strand, within GFF447 at 486.464 kb on + strand, within GFF448 at 486.464 kb on + strand, within GFF448 at 486.464 kb on + strand, within GFF448 at 486.465 kb on - strand, within GFF448 at 486.465 kb on - strand, within GFF448 at 486.465 kb on - strand, within GFF448 at 486.465 kb on - strand, within GFF448 at 486.465 kb on - strand, within GFF448 at 486.606 kb on + strand at 486.606 kb on + strand at 486.607 kb on - strand at 486.607 kb on - strand at 486.607 kb on - strand at 486.607 kb on - strand at 486.607 kb on - strand at 486.695 kb on + strand at 486.699 kb on + strand at 486.699 kb on + strand at 486.699 kb on + strand at 486.700 kb on - strand at 486.700 kb on - strand at 486.726 kb on + strand at 486.726 kb on + strand at 486.726 kb on + strand at 486.726 kb on + strand at 486.726 kb on + strand at 486.726 kb on + strand at 486.726 kb on + strand at 486.727 kb on - strand at 486.727 kb on - strand at 486.727 kb on - strand at 486.727 kb on - strand at 486.727 kb on - strand at 486.727 kb on - strand at 486.819 kb on + strand, within GFF449 at 486.819 kb on + strand, within GFF449 at 486.820 kb on - strand, within GFF449 at 486.820 kb on - strand, within GFF449 at 486.820 kb on - strand, within GFF449 at 486.820 kb on - strand, within GFF449 at 487.210 kb on - strand, within GFF449 at 487.585 kb on - strand, within GFF449
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 485,367 + GFF447 0.25 -0.5 485,367 + GFF447 0.25 +0.3 485,367 + GFF447 0.25 -1.3 485,368 - GFF447 0.25 +0.9 485,368 - GFF447 0.25 -0.7 485,368 - GFF447 0.25 +0.8 485,368 - GFF447 0.25 +0.1 485,497 - GFF447 0.40 +0.1 485,497 - GFF447 0.40 -1.8 486,464 + GFF448 0.74 -1.0 486,464 + GFF448 0.74 -0.7 486,464 + GFF448 0.74 -1.7 486,465 - GFF448 0.74 -0.7 486,465 - GFF448 0.74 +0.7 486,465 - GFF448 0.74 -0.3 486,465 - GFF448 0.74 -0.3 486,465 - GFF448 0.74 -0.3 486,606 + +0.3 486,606 + +1.3 486,607 - -0.8 486,607 - -0.0 486,607 - +0.0 486,607 - -0.1 486,607 - +1.0 486,695 + +0.0 486,699 + +0.3 486,699 + -0.8 486,699 + +0.2 486,700 - +1.3 486,700 - -0.3 486,726 + -1.5 486,726 + -0.8 486,726 + +1.3 486,726 + -1.1 486,726 + -0.5 486,726 + -0.4 486,726 + -2.0 486,727 - +0.0 486,727 - +0.4 486,727 - -1.3 486,727 - +1.5 486,727 - -0.3 486,727 - +0.2 486,819 + GFF449 0.15 -1.8 486,819 + GFF449 0.15 -0.4 486,820 - GFF449 0.15 -0.7 486,820 - GFF449 0.15 +0.5 486,820 - GFF449 0.15 +0.9 486,820 - GFF449 0.15 +0.4 487,210 - GFF449 0.43 +2.3 487,585 - GFF449 0.69 -0.5
Or see this region's nucleotide sequence