Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4214 and GFF4215 are separated by 2 nucleotides GFF4215 and GFF4216 are separated by 87 nucleotides GFF4216 and GFF4217 are separated by 159 nucleotides
GFF4214 - L-amino acid ABC transporter (Glu/Asp/His/...), permease protein 2 AapM, at 129,064 to 130,173
GFF4214
GFF4215 - L-amino acid ABC transporter (Glu/Asp/His/...), permease protein 1 AapQ, at 130,176 to 130,856
GFF4215
GFF4216 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 130,944 to 131,288
GFF4216
GFF4217 - Glutamate/aspartate ABC transporter, substrate-binding protein GltI (TC 3.A.1.3.4), at 131,448 to 132,464
GFF4217
Position (kb) 130
131
132 Strain fitness (log2 ratio) -1
0
1 at 130.208 kb on - strand at 130.379 kb on - strand, within GFF4215 at 130.543 kb on + strand, within GFF4215 at 130.543 kb on + strand, within GFF4215 at 130.972 kb on + strand at 131.020 kb on - strand, within GFF4216 at 131.282 kb on - strand at 131.390 kb on + strand at 131.391 kb on - strand at 131.391 kb on - strand at 131.467 kb on + strand at 131.467 kb on + strand at 131.467 kb on + strand at 131.468 kb on - strand at 131.468 kb on - strand at 131.488 kb on + strand at 131.489 kb on - strand at 131.568 kb on + strand, within GFF4217 at 131.587 kb on + strand, within GFF4217 at 131.588 kb on - strand, within GFF4217 at 131.588 kb on - strand, within GFF4217 at 131.588 kb on - strand, within GFF4217 at 131.707 kb on + strand, within GFF4217 at 131.707 kb on + strand, within GFF4217
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 130,208 - +1.7 130,379 - GFF4215 0.30 +0.2 130,543 + GFF4215 0.54 -0.3 130,543 + GFF4215 0.54 +0.5 130,972 + +0.3 131,020 - GFF4216 0.22 -1.2 131,282 - -0.3 131,390 + +0.5 131,391 - -1.2 131,391 - +0.5 131,467 + -0.0 131,467 + -0.6 131,467 + +0.4 131,468 - -0.9 131,468 - -0.2 131,488 + -0.2 131,489 - -0.2 131,568 + GFF4217 0.12 +0.5 131,587 + GFF4217 0.14 -0.3 131,588 - GFF4217 0.14 +1.2 131,588 - GFF4217 0.14 -0.1 131,588 - GFF4217 0.14 +0.3 131,707 + GFF4217 0.25 +0.2 131,707 + GFF4217 0.25 -1.6
Or see this region's nucleotide sequence