Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4154 and GFF4155 overlap by 4 nucleotides GFF4155 and GFF4156 overlap by 14 nucleotides GFF4156 and GFF4157 overlap by 4 nucleotides GFF4157 and GFF4158 are separated by 10 nucleotides
GFF4154 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 60,517 to 61,371
GFF4154
GFF4155 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 61,368 to 62,138
GFF4155
GFF4156 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 62,125 to 62,865
GFF4156
GFF4157 - hypothetical protein, at 62,862 to 63,812
GFF4157
GFF4158 - hypothetical protein, at 63,823 to 64,251
GFF4158
Position (kb) 62
63 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 61.255 kb on + strand, within GFF4154 at 62.104 kb on - strand at 62.104 kb on - strand at 62.428 kb on + strand, within GFF4156 at 62.429 kb on - strand, within GFF4156 at 62.641 kb on + strand, within GFF4156 at 62.642 kb on - strand, within GFF4156 at 62.734 kb on + strand, within GFF4156 at 62.734 kb on + strand, within GFF4156 at 62.887 kb on - strand at 62.887 kb on - strand at 62.994 kb on + strand, within GFF4157 at 62.995 kb on - strand, within GFF4157 at 62.995 kb on - strand, within GFF4157
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 61,255 + GFF4154 0.86 +3.3 62,104 - -0.9 62,104 - -0.4 62,428 + GFF4156 0.41 +0.9 62,429 - GFF4156 0.41 +0.5 62,641 + GFF4156 0.70 +0.4 62,642 - GFF4156 0.70 +1.1 62,734 + GFF4156 0.82 -0.7 62,734 + GFF4156 0.82 -2.2 62,887 - -0.5 62,887 - -0.1 62,994 + GFF4157 0.14 +0.5 62,995 - GFF4157 0.14 -1.0 62,995 - GFF4157 0.14 -0.4
Or see this region's nucleotide sequence