Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4150 and GFF4151 are separated by 68 nucleotides GFF4151 and GFF4152 are separated by 2 nucleotides GFF4152 and GFF4153 are separated by 28 nucleotides GFF4153 and GFF4154 overlap by 28 nucleotides
GFF4150 - Salicylate hydroxylase (EC 1.14.13.1), at 56,448 to 57,704
GFF4150
GFF4151 - RidA/YER057c/UK114 superfamily, group 1, at 57,773 to 58,405
GFF4151
GFF4152 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 58,408 to 59,574
GFF4152
GFF4153 - ABC transporter, permease protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 59,603 to 60,544
GFF4153
GFF4154 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 60,517 to 61,371
GFF4154
Position (kb) 58
59
60 Strain fitness (log2 ratio) -2
-1
0
1
2 at 57.424 kb on - strand, within GFF4150 at 57.424 kb on - strand, within GFF4150 at 57.471 kb on + strand, within GFF4150 at 57.684 kb on + strand at 57.684 kb on + strand at 57.814 kb on - strand at 57.962 kb on + strand, within GFF4151 at 57.963 kb on - strand, within GFF4151 at 57.963 kb on - strand, within GFF4151 at 57.995 kb on + strand, within GFF4151 at 57.995 kb on + strand, within GFF4151 at 58.166 kb on + strand, within GFF4151 at 58.167 kb on - strand, within GFF4151 at 58.167 kb on - strand, within GFF4151 at 58.184 kb on + strand, within GFF4151 at 58.185 kb on - strand, within GFF4151 at 58.185 kb on - strand, within GFF4151 at 58.241 kb on + strand, within GFF4151 at 58.645 kb on + strand, within GFF4152 at 58.786 kb on + strand, within GFF4152 at 58.787 kb on - strand, within GFF4152 at 58.787 kb on - strand, within GFF4152 at 58.834 kb on + strand, within GFF4152 at 58.834 kb on + strand, within GFF4152 at 58.835 kb on - strand, within GFF4152 at 58.835 kb on - strand, within GFF4152 at 59.014 kb on + strand, within GFF4152 at 59.089 kb on + strand, within GFF4152 at 59.089 kb on + strand, within GFF4152 at 59.105 kb on - strand, within GFF4152 at 59.195 kb on - strand, within GFF4152 at 59.195 kb on - strand, within GFF4152 at 59.654 kb on + strand at 59.654 kb on + strand at 59.705 kb on + strand, within GFF4153 at 59.850 kb on - strand, within GFF4153 at 59.960 kb on + strand, within GFF4153 at 59.960 kb on + strand, within GFF4153 at 60.014 kb on + strand, within GFF4153 at 60.269 kb on + strand, within GFF4153 at 60.269 kb on + strand, within GFF4153 at 60.270 kb on - strand, within GFF4153 at 60.526 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 57,424 - GFF4150 0.78 +0.1 57,424 - GFF4150 0.78 -0.3 57,471 + GFF4150 0.81 +0.3 57,684 + +0.1 57,684 + -1.1 57,814 - -0.6 57,962 + GFF4151 0.30 -0.1 57,963 - GFF4151 0.30 +0.0 57,963 - GFF4151 0.30 -0.6 57,995 + GFF4151 0.35 +1.1 57,995 + GFF4151 0.35 -0.1 58,166 + GFF4151 0.62 +0.4 58,167 - GFF4151 0.62 +0.6 58,167 - GFF4151 0.62 -0.1 58,184 + GFF4151 0.65 -0.3 58,185 - GFF4151 0.65 -1.8 58,185 - GFF4151 0.65 -0.2 58,241 + GFF4151 0.74 +0.7 58,645 + GFF4152 0.20 -0.4 58,786 + GFF4152 0.32 -0.3 58,787 - GFF4152 0.32 -1.8 58,787 - GFF4152 0.32 -0.9 58,834 + GFF4152 0.37 +0.4 58,834 + GFF4152 0.37 -2.1 58,835 - GFF4152 0.37 -0.3 58,835 - GFF4152 0.37 -1.5 59,014 + GFF4152 0.52 +0.4 59,089 + GFF4152 0.58 -1.1 59,089 + GFF4152 0.58 -0.2 59,105 - GFF4152 0.60 -0.6 59,195 - GFF4152 0.67 -1.8 59,195 - GFF4152 0.67 +0.3 59,654 + -1.9 59,654 + -0.5 59,705 + GFF4153 0.11 -1.8 59,850 - GFF4153 0.26 -0.9 59,960 + GFF4153 0.38 +2.0 59,960 + GFF4153 0.38 -0.8 60,014 + GFF4153 0.44 -0.5 60,269 + GFF4153 0.71 -1.1 60,269 + GFF4153 0.71 -0.4 60,270 - GFF4153 0.71 -0.6 60,526 + -1.1
Or see this region's nucleotide sequence