Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4132 and GFF4133 are separated by 103 nucleotides GFF4133 and GFF4134 are separated by 46 nucleotides
GFF4132 - Acyl-CoA dehydrogenase; probable dibenzothiophene desulfurization enzyme, at 38,268 to 39,500
GFF4132
GFF4133 - Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases; sulfonate monooxygenase, at 39,604 to 40,788
GFF4133
GFF4134 - Small molecule metabolism, at 40,835 to 41,869
GFF4134
Position (kb) 39
40
41 Strain fitness (log2 ratio) -2
-1
0
1
2 at 38.745 kb on + strand, within GFF4132 at 38.746 kb on - strand, within GFF4132 at 38.746 kb on - strand, within GFF4132 at 39.054 kb on + strand, within GFF4132 at 39.055 kb on - strand, within GFF4132 at 39.055 kb on - strand, within GFF4132 at 39.596 kb on - strand at 39.596 kb on - strand at 39.791 kb on - strand, within GFF4133 at 40.129 kb on + strand, within GFF4133 at 40.492 kb on + strand, within GFF4133 at 40.816 kb on + strand at 40.816 kb on + strand at 40.817 kb on - strand at 41.001 kb on - strand, within GFF4134 at 41.001 kb on - strand, within GFF4134 at 41.110 kb on + strand, within GFF4134 at 41.198 kb on + strand, within GFF4134 at 41.198 kb on + strand, within GFF4134 at 41.285 kb on + strand, within GFF4134 at 41.381 kb on + strand, within GFF4134 at 41.382 kb on - strand, within GFF4134 at 41.382 kb on - strand, within GFF4134 at 41.433 kb on - strand, within GFF4134 at 41.436 kb on - strand, within GFF4134 at 41.436 kb on - strand, within GFF4134 at 41.456 kb on + strand, within GFF4134 at 41.456 kb on + strand, within GFF4134 at 41.720 kb on + strand, within GFF4134 at 41.721 kb on - strand, within GFF4134 at 41.739 kb on - strand, within GFF4134
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 38,745 + GFF4132 0.39 -2.3 38,746 - GFF4132 0.39 -0.2 38,746 - GFF4132 0.39 -1.4 39,054 + GFF4132 0.64 -0.4 39,055 - GFF4132 0.64 +0.6 39,055 - GFF4132 0.64 -1.0 39,596 - +0.1 39,596 - -1.6 39,791 - GFF4133 0.16 -1.9 40,129 + GFF4133 0.44 -0.6 40,492 + GFF4133 0.75 -1.7 40,816 + -0.6 40,816 + +1.1 40,817 - +1.0 41,001 - GFF4134 0.16 +1.7 41,001 - GFF4134 0.16 -0.1 41,110 + GFF4134 0.27 -0.3 41,198 + GFF4134 0.35 -0.1 41,198 + GFF4134 0.35 -1.7 41,285 + GFF4134 0.43 +1.5 41,381 + GFF4134 0.53 -0.0 41,382 - GFF4134 0.53 +0.2 41,382 - GFF4134 0.53 +2.1 41,433 - GFF4134 0.58 -1.1 41,436 - GFF4134 0.58 -0.6 41,436 - GFF4134 0.58 +1.0 41,456 + GFF4134 0.60 +1.7 41,456 + GFF4134 0.60 +2.0 41,720 + GFF4134 0.86 +0.1 41,721 - GFF4134 0.86 -1.6 41,739 - GFF4134 0.87 -0.0
Or see this region's nucleotide sequence