Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4070 and GFF4071 are separated by 67 nucleotides GFF4071 and GFF4072 are separated by 116 nucleotides GFF4072 and GFF4073 overlap by 4 nucleotides GFF4073 and GFF4074 are separated by 35 nucleotides
GFF4070 - no description, at 285,317 to 285,712
GFF4070
GFF4071 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 285,780 to 286,205
GFF4071
GFF4072 - hypothetical protein, at 286,322 to 286,693
GFF4072
GFF4073 - Copper tolerance protein, at 286,690 to 288,114
GFF4073
GFF4074 - Multicopper oxidase, at 288,150 to 289,565
GFF4074
Position (kb) 286
287
288
289 Strain fitness (log2 ratio) -1
0
1
2 at 285.767 kb on + strand at 285.768 kb on - strand at 286.059 kb on - strand, within GFF4071 at 286.203 kb on + strand at 286.204 kb on - strand at 286.293 kb on + strand at 286.469 kb on + strand, within GFF4072 at 286.469 kb on + strand, within GFF4072 at 286.469 kb on + strand, within GFF4072 at 286.490 kb on + strand, within GFF4072 at 286.491 kb on - strand, within GFF4072 at 286.571 kb on + strand, within GFF4072 at 287.230 kb on + strand, within GFF4073 at 287.230 kb on + strand, within GFF4073 at 287.231 kb on - strand, within GFF4073 at 287.812 kb on + strand, within GFF4073 at 288.309 kb on + strand, within GFF4074 at 288.660 kb on + strand, within GFF4074 at 288.661 kb on - strand, within GFF4074 at 289.026 kb on + strand, within GFF4074 at 289.026 kb on + strand, within GFF4074
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 285,767 + +0.7 285,768 - -1.0 286,059 - GFF4071 0.65 -0.3 286,203 + +0.4 286,204 - +0.6 286,293 + -0.0 286,469 + GFF4072 0.40 -0.4 286,469 + GFF4072 0.40 -0.4 286,469 + GFF4072 0.40 -1.0 286,490 + GFF4072 0.45 -0.1 286,491 - GFF4072 0.45 -0.1 286,571 + GFF4072 0.67 -1.1 287,230 + GFF4073 0.38 -1.2 287,230 + GFF4073 0.38 -1.6 287,231 - GFF4073 0.38 -0.6 287,812 + GFF4073 0.79 +2.0 288,309 + GFF4074 0.11 -0.4 288,660 + GFF4074 0.36 -0.7 288,661 - GFF4074 0.36 +0.2 289,026 + GFF4074 0.62 -0.6 289,026 + GFF4074 0.62 -0.4
Or see this region's nucleotide sequence