Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4062 and GFF4063 are separated by 12 nucleotides GFF4063 and GFF4064 are separated by 202 nucleotides GFF4064 and GFF4065 are separated by 138 nucleotides GFF4065 and GFF4066 are separated by 78 nucleotides
GFF4062 - Copper resistance protein B, at 277,330 to 278,247
GFF4062
GFF4063 - Cytochrome c family protein, at 278,260 to 278,799
GFF4063
GFF4064 - no description, at 279,002 to 279,340
GFF4064
GFF4065 - Uncharacterized cysteine-rich DUF326 protein bsYhjQ/STM1261, at 279,479 to 279,808
GFF4065
GFF4066 - Putative type II restriction enzyme NmeDIP (EC 3.1.21.4) (Endonuclease NmeDIP) (R.NmeDIP), at 279,887 to 281,173
GFF4066
Position (kb) 279
280 Strain fitness (log2 ratio) -2
-1
0
1
2 at 278.029 kb on - strand, within GFF4062 at 278.035 kb on + strand, within GFF4062 at 278.035 kb on + strand, within GFF4062 at 278.135 kb on - strand, within GFF4062 at 278.135 kb on - strand, within GFF4062 at 278.180 kb on - strand at 278.180 kb on - strand at 278.353 kb on + strand, within GFF4063 at 278.353 kb on + strand, within GFF4063 at 278.354 kb on - strand, within GFF4063 at 278.501 kb on - strand, within GFF4063 at 278.843 kb on + strand at 278.876 kb on + strand at 278.876 kb on + strand at 278.876 kb on + strand at 278.877 kb on - strand at 278.877 kb on - strand at 278.913 kb on - strand at 278.917 kb on + strand at 278.917 kb on + strand at 278.918 kb on - strand at 278.918 kb on - strand at 278.918 kb on - strand at 278.943 kb on - strand at 278.943 kb on - strand at 278.964 kb on - strand at 279.056 kb on + strand, within GFF4064 at 279.338 kb on + strand at 279.494 kb on + strand at 279.494 kb on + strand at 279.495 kb on - strand at 279.495 kb on - strand at 279.495 kb on - strand at 279.497 kb on - strand at 279.582 kb on - strand, within GFF4065 at 279.678 kb on + strand, within GFF4065 at 279.678 kb on + strand, within GFF4065 at 279.678 kb on + strand, within GFF4065 at 279.678 kb on + strand, within GFF4065 at 279.679 kb on - strand, within GFF4065 at 279.679 kb on - strand, within GFF4065 at 279.679 kb on - strand, within GFF4065 at 279.679 kb on - strand, within GFF4065 at 279.679 kb on - strand, within GFF4065 at 279.679 kb on - strand, within GFF4065 at 279.826 kb on + strand at 279.897 kb on + strand at 279.897 kb on + strand at 279.973 kb on - strand at 279.981 kb on + strand at 280.064 kb on + strand, within GFF4066 at 280.137 kb on + strand, within GFF4066 at 280.137 kb on + strand, within GFF4066 at 280.138 kb on - strand, within GFF4066 at 280.138 kb on - strand, within GFF4066 at 280.294 kb on - strand, within GFF4066 at 280.294 kb on - strand, within GFF4066 at 280.294 kb on - strand, within GFF4066
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 278,029 - GFF4062 0.76 +1.6 278,035 + GFF4062 0.77 +0.0 278,035 + GFF4062 0.77 -1.2 278,135 - GFF4062 0.88 -0.2 278,135 - GFF4062 0.88 -0.5 278,180 - +0.7 278,180 - +0.6 278,353 + GFF4063 0.17 -1.2 278,353 + GFF4063 0.17 -0.6 278,354 - GFF4063 0.17 -1.2 278,501 - GFF4063 0.45 +1.0 278,843 + -1.0 278,876 + -0.1 278,876 + +0.0 278,876 + +2.1 278,877 - -0.4 278,877 - -1.8 278,913 - -0.2 278,917 + +1.9 278,917 + +0.6 278,918 - -0.4 278,918 - -0.3 278,918 - +0.1 278,943 - -0.1 278,943 - +0.0 278,964 - +0.9 279,056 + GFF4064 0.16 -0.1 279,338 + -0.3 279,494 + +0.4 279,494 + -0.9 279,495 - +0.4 279,495 - -0.7 279,495 - -0.3 279,497 - -0.7 279,582 - GFF4065 0.31 -1.5 279,678 + GFF4065 0.60 +1.0 279,678 + GFF4065 0.60 +0.4 279,678 + GFF4065 0.60 -0.4 279,678 + GFF4065 0.60 +0.2 279,679 - GFF4065 0.61 +0.9 279,679 - GFF4065 0.61 -0.4 279,679 - GFF4065 0.61 +0.3 279,679 - GFF4065 0.61 -0.0 279,679 - GFF4065 0.61 -0.1 279,679 - GFF4065 0.61 +2.2 279,826 + -0.1 279,897 + -0.3 279,897 + -0.4 279,973 - -0.4 279,981 + -1.0 280,064 + GFF4066 0.14 +0.2 280,137 + GFF4066 0.19 +0.4 280,137 + GFF4066 0.19 +1.6 280,138 - GFF4066 0.20 -0.3 280,138 - GFF4066 0.20 +0.8 280,294 - GFF4066 0.32 -0.2 280,294 - GFF4066 0.32 +1.4 280,294 - GFF4066 0.32 -0.3
Or see this region's nucleotide sequence