Strain Fitness in Variovorax sp. SCN45 around GFF4005

Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4004 and GFF4005 are separated by 58 nucleotidesGFF4005 and GFF4006 are separated by 97 nucleotides GFF4004 - Homoserine kinase (EC 2.7.1.39), at 209,477 to 210,541 GFF4004 GFF4005 - hypothetical protein, at 210,600 to 210,977 GFF4005 GFF4006 - DNA polymerase I (EC 2.7.7.7), at 211,075 to 213,891 GFF4006 Position (kb) 210 211Strain fitness (log2 ratio) -1 0 1at 210.694 kb on + strand, within GFF4005at 210.695 kb on - strand, within GFF4005at 210.695 kb on - strand, within GFF4005at 210.971 kb on + strandat 210.971 kb on + strandat 210.971 kb on + strandat 210.972 kb on - strandat 210.972 kb on - strandat 210.972 kb on - strandat 210.977 kb on + strandat 210.977 kb on + strandat 210.977 kb on + strandat 210.978 kb on - strandat 210.978 kb on - strandat 210.978 kb on - strandat 210.978 kb on - strandat 210.988 kb on + strandat 210.988 kb on + strandat 210.988 kb on + strandat 210.988 kb on + strandat 210.988 kb on + strandat 210.989 kb on - strandat 210.989 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
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210,694 + GFF4005 0.25 +0.0
210,695 - GFF4005 0.25 -1.7
210,695 - GFF4005 0.25 -0.4
210,971 + +0.8
210,971 + -0.3
210,971 + -0.3
210,972 - +0.2
210,972 - +0.3
210,972 - -0.2
210,977 + +1.3
210,977 + -0.6
210,977 + -0.4
210,978 - +0.0
210,978 - -1.0
210,978 - -1.1
210,978 - -0.7
210,988 + +1.6
210,988 + -0.3
210,988 + -1.0
210,988 + +0.8
210,988 + -1.5
210,989 - -0.5
210,989 - +0.5

Or see this region's nucleotide sequence