Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3944 and GFF3945 are separated by 123 nucleotides GFF3945 and GFF3946 are separated by 13 nucleotides GFF3946 and GFF3947 are separated by 19 nucleotides GFF3947 and GFF3948 are separated by 7 nucleotides
GFF3944 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3), at 148,132 to 148,770
GFF3944
GFF3945 - Phosphate transport regulator (distant homolog of PhoU), at 148,894 to 149,538
GFF3945
GFF3946 - Probable low-affinity inorganic phosphate transporter, at 149,552 to 150,562
GFF3946
GFF3947 - Putative transmembrane protein, at 150,582 to 151,028
GFF3947
GFF3948 - Acetyltransferase, GNAT family, at 151,036 to 151,560
GFF3948
Position (kb) 149
150
151 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 148.820 kb on + strand at 148.820 kb on + strand at 148.820 kb on + strand at 148.820 kb on + strand at 148.821 kb on - strand at 148.821 kb on - strand at 149.002 kb on + strand, within GFF3945 at 149.002 kb on + strand, within GFF3945 at 149.002 kb on + strand, within GFF3945 at 149.002 kb on + strand, within GFF3945 at 149.002 kb on + strand, within GFF3945 at 149.002 kb on + strand, within GFF3945 at 149.002 kb on + strand, within GFF3945 at 149.002 kb on + strand, within GFF3945 at 149.002 kb on + strand, within GFF3945 at 149.003 kb on - strand, within GFF3945 at 149.003 kb on - strand, within GFF3945 at 149.003 kb on - strand, within GFF3945 at 149.029 kb on + strand, within GFF3945 at 149.029 kb on + strand, within GFF3945 at 149.029 kb on + strand, within GFF3945 at 149.029 kb on + strand, within GFF3945 at 149.029 kb on + strand, within GFF3945 at 149.030 kb on - strand, within GFF3945 at 149.030 kb on - strand, within GFF3945 at 149.030 kb on - strand, within GFF3945 at 149.030 kb on - strand, within GFF3945 at 149.030 kb on - strand, within GFF3945 at 149.030 kb on - strand, within GFF3945 at 149.030 kb on - strand, within GFF3945 at 149.030 kb on - strand, within GFF3945 at 149.030 kb on - strand, within GFF3945 at 149.030 kb on - strand, within GFF3945 at 149.030 kb on - strand, within GFF3945 at 149.206 kb on + strand, within GFF3945 at 149.207 kb on - strand, within GFF3945 at 149.207 kb on - strand, within GFF3945 at 149.458 kb on + strand, within GFF3945 at 149.458 kb on + strand, within GFF3945 at 149.458 kb on + strand, within GFF3945 at 149.458 kb on + strand, within GFF3945 at 149.458 kb on + strand, within GFF3945 at 149.458 kb on + strand, within GFF3945 at 149.458 kb on + strand, within GFF3945 at 149.458 kb on + strand, within GFF3945 at 149.459 kb on - strand, within GFF3945 at 149.459 kb on - strand, within GFF3945 at 149.459 kb on - strand, within GFF3945 at 149.459 kb on - strand, within GFF3945 at 149.459 kb on - strand, within GFF3945 at 149.459 kb on - strand, within GFF3945 at 149.586 kb on + strand at 149.586 kb on + strand at 149.586 kb on + strand at 149.586 kb on + strand at 149.586 kb on + strand at 149.586 kb on + strand at 149.586 kb on + strand at 149.587 kb on - strand at 149.587 kb on - strand at 149.587 kb on - strand at 149.587 kb on - strand at 149.587 kb on - strand at 149.856 kb on - strand, within GFF3946 at 149.856 kb on - strand, within GFF3946 at 149.856 kb on - strand, within GFF3946 at 149.856 kb on - strand, within GFF3946 at 149.856 kb on - strand, within GFF3946 at 149.858 kb on + strand, within GFF3946 at 149.858 kb on + strand, within GFF3946 at 149.858 kb on + strand, within GFF3946 at 149.858 kb on + strand, within GFF3946 at 149.858 kb on + strand, within GFF3946 at 149.858 kb on + strand, within GFF3946 at 149.858 kb on + strand, within GFF3946 at 149.859 kb on - strand, within GFF3946 at 149.859 kb on - strand, within GFF3946 at 149.859 kb on - strand, within GFF3946 at 150.110 kb on + strand, within GFF3946 at 150.110 kb on + strand, within GFF3946 at 150.110 kb on + strand, within GFF3946 at 150.110 kb on + strand, within GFF3946 at 150.111 kb on - strand, within GFF3946 at 150.111 kb on - strand, within GFF3946 at 150.111 kb on - strand, within GFF3946 at 150.111 kb on - strand, within GFF3946 at 150.185 kb on + strand, within GFF3946 at 150.185 kb on + strand, within GFF3946 at 150.186 kb on - strand, within GFF3946 at 150.186 kb on - strand, within GFF3946 at 150.186 kb on - strand, within GFF3946 at 150.236 kb on + strand, within GFF3946 at 150.409 kb on + strand, within GFF3946 at 150.536 kb on + strand at 150.536 kb on + strand at 150.536 kb on + strand at 150.537 kb on - strand at 150.625 kb on + strand at 150.874 kb on + strand, within GFF3947 at 150.874 kb on + strand, within GFF3947 at 150.874 kb on + strand, within GFF3947 at 150.874 kb on + strand, within GFF3947 at 150.874 kb on + strand, within GFF3947 at 150.875 kb on - strand, within GFF3947 at 150.875 kb on - strand, within GFF3947 at 150.875 kb on - strand, within GFF3947 at 150.875 kb on - strand, within GFF3947 at 150.875 kb on - strand, within GFF3947 at 150.875 kb on - strand, within GFF3947 at 150.898 kb on + strand, within GFF3947 at 150.898 kb on + strand, within GFF3947 at 150.899 kb on - strand, within GFF3947 at 150.899 kb on - strand, within GFF3947 at 150.899 kb on - strand, within GFF3947 at 150.961 kb on + strand, within GFF3947 at 150.961 kb on + strand, within GFF3947 at 150.962 kb on - strand, within GFF3947 at 150.962 kb on - strand, within GFF3947 at 150.962 kb on - strand, within GFF3947 at 150.962 kb on - strand, within GFF3947 at 150.962 kb on - strand, within GFF3947 at 151.083 kb on - strand at 151.084 kb on + strand at 151.084 kb on + strand at 151.084 kb on + strand at 151.084 kb on + strand at 151.084 kb on + strand at 151.085 kb on - strand at 151.085 kb on - strand at 151.085 kb on - strand at 151.085 kb on - strand at 151.085 kb on - strand at 151.085 kb on - strand at 151.085 kb on - strand at 151.085 kb on - strand at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.325 kb on + strand, within GFF3948 at 151.326 kb on - strand, within GFF3948 at 151.397 kb on + strand, within GFF3948 at 151.397 kb on + strand, within GFF3948 at 151.397 kb on + strand, within GFF3948 at 151.398 kb on - strand, within GFF3948 at 151.398 kb on - strand, within GFF3948 at 151.502 kb on + strand, within GFF3948 at 151.502 kb on + strand, within GFF3948 at 151.502 kb on + strand, within GFF3948 at 151.502 kb on + strand, within GFF3948 at 151.502 kb on + strand, within GFF3948 at 151.502 kb on + strand, within GFF3948 at 151.502 kb on + strand, within GFF3948 at 151.502 kb on + strand, within GFF3948 at 151.503 kb on - strand, within GFF3948 at 151.503 kb on - strand, within GFF3948 at 151.503 kb on - strand, within GFF3948 at 151.503 kb on - strand, within GFF3948 at 151.503 kb on - strand, within GFF3948 at 151.503 kb on - strand, within GFF3948 at 151.503 kb on - strand, within GFF3948 at 151.503 kb on - strand, within GFF3948 at 151.503 kb on - strand, within GFF3948 at 151.503 kb on - strand, within GFF3948 at 151.503 kb on - strand, within GFF3948 at 151.503 kb on - strand, within GFF3948
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 148,820 + -0.5 148,820 + -0.5 148,820 + +0.3 148,820 + +0.0 148,821 - -1.2 148,821 - +3.2 149,002 + GFF3945 0.17 -0.3 149,002 + GFF3945 0.17 -1.2 149,002 + GFF3945 0.17 +0.4 149,002 + GFF3945 0.17 -1.5 149,002 + GFF3945 0.17 -1.2 149,002 + GFF3945 0.17 -0.7 149,002 + GFF3945 0.17 -0.8 149,002 + GFF3945 0.17 -0.1 149,002 + GFF3945 0.17 +0.3 149,003 - GFF3945 0.17 -1.9 149,003 - GFF3945 0.17 -0.0 149,003 - GFF3945 0.17 +0.0 149,029 + GFF3945 0.21 -0.6 149,029 + GFF3945 0.21 -0.1 149,029 + GFF3945 0.21 +0.3 149,029 + GFF3945 0.21 -0.2 149,029 + GFF3945 0.21 -0.2 149,030 - GFF3945 0.21 -1.2 149,030 - GFF3945 0.21 +0.4 149,030 - GFF3945 0.21 -0.6 149,030 - GFF3945 0.21 +1.0 149,030 - GFF3945 0.21 +0.0 149,030 - GFF3945 0.21 -0.7 149,030 - GFF3945 0.21 -1.7 149,030 - GFF3945 0.21 -1.8 149,030 - GFF3945 0.21 -0.2 149,030 - GFF3945 0.21 -0.4 149,030 - GFF3945 0.21 -0.8 149,206 + GFF3945 0.48 -0.4 149,207 - GFF3945 0.49 -2.2 149,207 - GFF3945 0.49 -1.8 149,458 + GFF3945 0.87 -0.6 149,458 + GFF3945 0.87 +0.9 149,458 + GFF3945 0.87 +0.6 149,458 + GFF3945 0.87 +0.1 149,458 + GFF3945 0.87 +0.2 149,458 + GFF3945 0.87 -0.6 149,458 + GFF3945 0.87 -1.0 149,458 + GFF3945 0.87 -0.2 149,459 - GFF3945 0.88 -0.6 149,459 - GFF3945 0.88 +0.6 149,459 - GFF3945 0.88 -0.2 149,459 - GFF3945 0.88 -1.1 149,459 - GFF3945 0.88 -0.8 149,459 - GFF3945 0.88 -1.1 149,586 + -1.0 149,586 + -1.3 149,586 + -0.1 149,586 + +1.3 149,586 + +0.1 149,586 + -0.5 149,586 + -0.4 149,587 - -0.1 149,587 - -0.3 149,587 - -0.3 149,587 - +0.2 149,587 - -0.0 149,856 - GFF3946 0.30 +1.0 149,856 - GFF3946 0.30 -0.1 149,856 - GFF3946 0.30 -0.3 149,856 - GFF3946 0.30 -1.4 149,856 - GFF3946 0.30 -1.7 149,858 + GFF3946 0.30 +0.6 149,858 + GFF3946 0.30 -0.9 149,858 + GFF3946 0.30 +0.8 149,858 + GFF3946 0.30 -0.8 149,858 + GFF3946 0.30 -0.1 149,858 + GFF3946 0.30 +0.1 149,858 + GFF3946 0.30 -0.2 149,859 - GFF3946 0.30 -1.0 149,859 - GFF3946 0.30 -0.3 149,859 - GFF3946 0.30 -0.2 150,110 + GFF3946 0.55 -0.8 150,110 + GFF3946 0.55 -0.3 150,110 + GFF3946 0.55 +0.3 150,110 + GFF3946 0.55 +1.6 150,111 - GFF3946 0.55 +0.4 150,111 - GFF3946 0.55 -0.5 150,111 - GFF3946 0.55 -0.7 150,111 - GFF3946 0.55 -0.4 150,185 + GFF3946 0.63 -0.3 150,185 + GFF3946 0.63 +0.6 150,186 - GFF3946 0.63 +0.3 150,186 - GFF3946 0.63 -1.1 150,186 - GFF3946 0.63 -1.0 150,236 + GFF3946 0.68 -0.7 150,409 + GFF3946 0.85 -0.8 150,536 + -1.4 150,536 + +0.3 150,536 + -0.1 150,537 - -0.3 150,625 + -0.6 150,874 + GFF3947 0.65 -0.2 150,874 + GFF3947 0.65 +0.8 150,874 + GFF3947 0.65 +0.3 150,874 + GFF3947 0.65 -1.1 150,874 + GFF3947 0.65 -0.2 150,875 - GFF3947 0.66 -1.5 150,875 - GFF3947 0.66 +1.6 150,875 - GFF3947 0.66 +1.4 150,875 - GFF3947 0.66 -0.3 150,875 - GFF3947 0.66 -0.0 150,875 - GFF3947 0.66 -1.1 150,898 + GFF3947 0.71 -0.5 150,898 + GFF3947 0.71 -0.4 150,899 - GFF3947 0.71 +0.6 150,899 - GFF3947 0.71 +0.8 150,899 - GFF3947 0.71 +1.9 150,961 + GFF3947 0.85 -0.7 150,961 + GFF3947 0.85 -0.6 150,962 - GFF3947 0.85 +0.5 150,962 - GFF3947 0.85 +0.5 150,962 - GFF3947 0.85 +0.2 150,962 - GFF3947 0.85 +0.8 150,962 - GFF3947 0.85 -1.4 151,083 - +0.5 151,084 + -1.0 151,084 + +0.4 151,084 + +0.3 151,084 + +1.3 151,084 + -0.6 151,085 - -1.2 151,085 - -0.2 151,085 - -0.2 151,085 - -0.3 151,085 - +0.4 151,085 - -0.5 151,085 - -0.6 151,085 - -0.6 151,298 + GFF3948 0.50 -1.5 151,298 + GFF3948 0.50 -0.1 151,298 + GFF3948 0.50 -0.8 151,298 + GFF3948 0.50 +0.1 151,298 + GFF3948 0.50 +0.6 151,298 + GFF3948 0.50 +0.6 151,298 + GFF3948 0.50 +0.1 151,298 + GFF3948 0.50 -1.2 151,298 + GFF3948 0.50 +0.4 151,298 + GFF3948 0.50 -0.4 151,298 + GFF3948 0.50 +1.8 151,298 + GFF3948 0.50 -1.4 151,298 + GFF3948 0.50 +0.8 151,298 + GFF3948 0.50 +0.0 151,298 + GFF3948 0.50 +0.4 151,298 + GFF3948 0.50 -1.2 151,298 + GFF3948 0.50 +0.1 151,298 + GFF3948 0.50 +0.0 151,298 + GFF3948 0.50 -0.4 151,299 - GFF3948 0.50 -1.7 151,299 - GFF3948 0.50 +0.0 151,299 - GFF3948 0.50 -1.4 151,299 - GFF3948 0.50 +1.1 151,299 - GFF3948 0.50 +0.4 151,299 - GFF3948 0.50 +0.0 151,299 - GFF3948 0.50 -1.7 151,299 - GFF3948 0.50 +1.2 151,299 - GFF3948 0.50 -0.9 151,299 - GFF3948 0.50 -0.1 151,299 - GFF3948 0.50 +0.3 151,299 - GFF3948 0.50 +0.0 151,299 - GFF3948 0.50 +1.0 151,299 - GFF3948 0.50 +0.2 151,299 - GFF3948 0.50 -0.9 151,299 - GFF3948 0.50 -0.4 151,325 + GFF3948 0.55 -1.0 151,326 - GFF3948 0.55 -1.6 151,397 + GFF3948 0.69 +0.9 151,397 + GFF3948 0.69 +0.4 151,397 + GFF3948 0.69 -0.3 151,398 - GFF3948 0.69 -1.0 151,398 - GFF3948 0.69 -0.1 151,502 + GFF3948 0.89 -0.2 151,502 + GFF3948 0.89 -0.4 151,502 + GFF3948 0.89 -0.0 151,502 + GFF3948 0.89 -0.1 151,502 + GFF3948 0.89 -0.0 151,502 + GFF3948 0.89 +0.0 151,502 + GFF3948 0.89 -0.3 151,502 + GFF3948 0.89 -0.5 151,503 - GFF3948 0.89 -0.5 151,503 - GFF3948 0.89 -0.2 151,503 - GFF3948 0.89 -1.0 151,503 - GFF3948 0.89 -0.1 151,503 - GFF3948 0.89 -2.1 151,503 - GFF3948 0.89 +0.7 151,503 - GFF3948 0.89 -1.5 151,503 - GFF3948 0.89 +0.7 151,503 - GFF3948 0.89 -0.1 151,503 - GFF3948 0.89 +0.8 151,503 - GFF3948 0.89 -0.3 151,503 - GFF3948 0.89 -0.9
Or see this region's nucleotide sequence