Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3883 and GFF3884 are separated by 48 nucleotides GFF3884 and GFF3885 overlap by 4 nucleotides GFF3885 and GFF3886 are separated by 30 nucleotides
GFF3883 - Tryptophan synthase beta chain (EC 4.2.1.20), at 79,895 to 81,181
GFF3883
GFF3884 - Phosphoribosylanthranilate isomerase (EC 5.3.1.24), at 81,230 to 81,937
GFF3884
GFF3885 - tRNA pseudouridine(38-40) synthase (EC 5.4.99.12), at 81,934 to 82,746
GFF3885
GFF3886 - Putative transmembrane protein, at 82,777 to 85,605
GFF3886
Position (kb)
81
82
83 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 81.950 kb on + strand at 81.950 kb on + strand at 81.950 kb on + strand at 81.950 kb on + strand at 81.950 kb on + strand at 81.950 kb on + strand at 81.950 kb on + strand at 81.950 kb on + strand at 81.950 kb on + strand at 81.950 kb on + strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 82.007 kb on + strand at 82.008 kb on - strand at 82.025 kb on + strand, within GFF3885 at 82.025 kb on + strand, within GFF3885 at 82.415 kb on + strand, within GFF3885 at 82.415 kb on + strand, within GFF3885 at 82.415 kb on + strand, within GFF3885 at 82.415 kb on + strand, within GFF3885 at 82.415 kb on + strand, within GFF3885 at 82.415 kb on + strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.421 kb on + strand, within GFF3885 at 82.421 kb on + strand, within GFF3885 at 82.421 kb on + strand, within GFF3885 at 82.422 kb on - strand, within GFF3885 at 82.422 kb on - strand, within GFF3885 at 82.706 kb on + strand at 82.706 kb on + strand at 82.707 kb on - strand at 82.707 kb on - strand at 82.707 kb on - strand at 82.707 kb on - strand at 82.707 kb on - strand at 82.721 kb on + strand at 82.722 kb on - strand at 82.722 kb on - strand at 82.732 kb on + strand at 82.733 kb on - strand at 82.733 kb on - strand at 82.733 kb on - strand at 82.733 kb on - strand at 83.237 kb on + strand, within GFF3886 at 83.238 kb on - strand, within GFF3886 at 83.238 kb on - strand, within GFF3886 at 83.238 kb on - strand, within GFF3886 at 83.498 kb on + strand, within GFF3886 at 83.499 kb on - strand, within GFF3886 at 83.499 kb on - strand, within GFF3886 at 83.499 kb on - strand, within GFF3886 at 83.499 kb on - strand, within GFF3886 at 83.603 kb on + strand, within GFF3886 at 83.604 kb on - strand, within GFF3886 at 83.604 kb on - strand, within GFF3886 at 83.604 kb on - strand, within GFF3886 at 83.604 kb on - strand, within GFF3886 at 83.604 kb on - strand, within GFF3886 at 83.632 kb on + strand, within GFF3886 at 83.723 kb on + strand, within GFF3886 at 83.724 kb on - strand, within GFF3886 at 83.724 kb on - strand, within GFF3886 at 83.732 kb on + strand, within GFF3886 at 83.732 kb on + strand, within GFF3886 at 83.733 kb on - strand, within GFF3886 at 83.733 kb on - strand, within GFF3886 at 83.733 kb on - strand, within GFF3886 at 83.733 kb on - strand, within GFF3886 at 83.733 kb on - strand, within GFF3886 at 83.733 kb on - strand, within GFF3886
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 81,950 + +0.7 81,950 + +0.8 81,950 + +0.3 81,950 + +0.9 81,950 + +0.0 81,950 + -0.1 81,950 + +0.7 81,950 + -0.4 81,950 + -1.1 81,950 + +1.1 81,951 - -0.1 81,951 - +0.5 81,951 - +0.1 81,951 - -2.2 81,951 - +0.0 81,951 - +1.0 81,951 - +0.4 81,951 - +0.4 81,951 - +0.3 81,951 - -2.3 81,951 - -1.0 81,951 - +0.4 81,951 - +0.3 81,951 - -0.1 81,951 - +1.9 81,951 - -0.2 81,951 - +0.1 82,007 + -0.2 82,008 - -0.3 82,025 + GFF3885 0.11 -1.3 82,025 + GFF3885 0.11 -0.4 82,415 + GFF3885 0.59 -0.4 82,415 + GFF3885 0.59 -0.2 82,415 + GFF3885 0.59 -1.4 82,415 + GFF3885 0.59 -0.3 82,415 + GFF3885 0.59 +0.2 82,415 + GFF3885 0.59 +1.6 82,416 - GFF3885 0.59 -1.9 82,416 - GFF3885 0.59 +0.5 82,416 - GFF3885 0.59 -1.9 82,416 - GFF3885 0.59 -0.8 82,416 - GFF3885 0.59 -0.4 82,416 - GFF3885 0.59 -0.7 82,416 - GFF3885 0.59 -1.8 82,416 - GFF3885 0.59 -0.5 82,416 - GFF3885 0.59 -1.9 82,416 - GFF3885 0.59 +0.7 82,416 - GFF3885 0.59 -0.4 82,421 + GFF3885 0.60 -0.3 82,421 + GFF3885 0.60 -0.3 82,421 + GFF3885 0.60 -0.4 82,422 - GFF3885 0.60 -1.0 82,422 - GFF3885 0.60 -1.3 82,706 + -0.7 82,706 + +0.3 82,707 - +0.6 82,707 - +1.4 82,707 - -1.2 82,707 - +1.6 82,707 - -0.1 82,721 + -0.4 82,722 - -0.9 82,722 - +0.8 82,732 + -1.5 82,733 - +0.6 82,733 - -0.4 82,733 - -2.3 82,733 - -0.1 83,237 + GFF3886 0.16 +0.2 83,238 - GFF3886 0.16 -0.3 83,238 - GFF3886 0.16 +0.2 83,238 - GFF3886 0.16 +0.6 83,498 + GFF3886 0.25 -1.0 83,499 - GFF3886 0.26 -0.4 83,499 - GFF3886 0.26 -0.3 83,499 - GFF3886 0.26 -0.2 83,499 - GFF3886 0.26 +0.1 83,603 + GFF3886 0.29 -0.2 83,604 - GFF3886 0.29 -0.2 83,604 - GFF3886 0.29 -0.9 83,604 - GFF3886 0.29 -0.1 83,604 - GFF3886 0.29 -0.3 83,604 - GFF3886 0.29 -0.3 83,632 + GFF3886 0.30 -1.0 83,723 + GFF3886 0.33 +0.3 83,724 - GFF3886 0.33 -0.1 83,724 - GFF3886 0.33 +1.6 83,732 + GFF3886 0.34 -1.1 83,732 + GFF3886 0.34 +0.6 83,733 - GFF3886 0.34 +0.1 83,733 - GFF3886 0.34 -1.1 83,733 - GFF3886 0.34 +0.7 83,733 - GFF3886 0.34 +0.1 83,733 - GFF3886 0.34 -0.1 83,733 - GFF3886 0.34 +2.0
Or see this region's nucleotide sequence