Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3852 and GFF3853 overlap by 4 nucleotides GFF3853 and GFF3854 are separated by 82 nucleotides GFF3854 and GFF3855 are separated by 146 nucleotides GFF3855 and GFF3856 are separated by 61 nucleotides
GFF3852 - Tetrapyrrole methylase family protein, at 52,277 to 53,038
GFF3852
GFF3853 - Septum formation protein Maf, at 53,035 to 53,616
GFF3853
GFF3854 - FIG01269488: protein, clustered with ribosomal protein L32p, at 53,699 to 54,268
GFF3854
GFF3855 - LSU ribosomal protein L32p @ LSU ribosomal protein L32p, zinc-independent, at 54,415 to 54,597
GFF3855
GFF3856 - Phosphate:acyl-ACP acyltransferase PlsX (EC 2.3.1.n2), at 54,659 to 55,756
GFF3856
Position (kb) 53
54
55 Strain fitness (log2 ratio) -2
-1
0
1
2 at 52.817 kb on + strand, within GFF3852 at 52.817 kb on + strand, within GFF3852 at 52.818 kb on - strand, within GFF3852 at 52.818 kb on - strand, within GFF3852 at 52.818 kb on - strand, within GFF3852 at 52.884 kb on + strand, within GFF3852 at 52.884 kb on + strand, within GFF3852 at 52.884 kb on + strand, within GFF3852 at 52.884 kb on + strand, within GFF3852 at 52.884 kb on + strand, within GFF3852 at 52.885 kb on - strand, within GFF3852 at 52.885 kb on - strand, within GFF3852 at 53.019 kb on + strand at 53.019 kb on + strand at 53.019 kb on + strand at 53.019 kb on + strand at 53.019 kb on + strand at 53.019 kb on + strand at 53.020 kb on - strand at 53.020 kb on - strand at 53.020 kb on - strand at 53.020 kb on - strand at 53.020 kb on - strand at 53.020 kb on - strand at 53.686 kb on + strand at 53.686 kb on + strand at 53.686 kb on + strand at 53.789 kb on + strand, within GFF3854 at 53.789 kb on + strand, within GFF3854 at 53.789 kb on + strand, within GFF3854 at 53.789 kb on + strand, within GFF3854 at 53.790 kb on - strand, within GFF3854 at 54.266 kb on + strand at 54.266 kb on + strand at 54.267 kb on - strand at 54.289 kb on + strand at 54.289 kb on + strand at 54.290 kb on - strand at 54.627 kb on + strand at 54.627 kb on + strand at 54.627 kb on + strand at 54.627 kb on + strand at 54.627 kb on + strand at 54.627 kb on + strand at 54.627 kb on + strand at 54.627 kb on + strand at 54.628 kb on - strand at 54.628 kb on - strand at 54.628 kb on - strand at 54.628 kb on - strand at 54.628 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 52,817 + GFF3852 0.71 +0.1 52,817 + GFF3852 0.71 +0.1 52,818 - GFF3852 0.71 -0.4 52,818 - GFF3852 0.71 -0.1 52,818 - GFF3852 0.71 -0.5 52,884 + GFF3852 0.80 +0.7 52,884 + GFF3852 0.80 +0.4 52,884 + GFF3852 0.80 -1.5 52,884 + GFF3852 0.80 -0.2 52,884 + GFF3852 0.80 -0.4 52,885 - GFF3852 0.80 -0.1 52,885 - GFF3852 0.80 +0.5 53,019 + -0.3 53,019 + +2.6 53,019 + -0.2 53,019 + -1.1 53,019 + +1.2 53,019 + -0.3 53,020 - -0.2 53,020 - +0.3 53,020 - -0.3 53,020 - -0.7 53,020 - +0.5 53,020 - -0.5 53,686 + +0.1 53,686 + -0.7 53,686 + +0.1 53,789 + GFF3854 0.16 -1.4 53,789 + GFF3854 0.16 -0.4 53,789 + GFF3854 0.16 -0.1 53,789 + GFF3854 0.16 -2.2 53,790 - GFF3854 0.16 -1.5 54,266 + +0.3 54,266 + +1.2 54,267 - -2.4 54,289 + +0.3 54,289 + +0.0 54,290 - -0.4 54,627 + +0.2 54,627 + +0.1 54,627 + +0.3 54,627 + -0.5 54,627 + -0.8 54,627 + -1.0 54,627 + +0.1 54,627 + -0.7 54,628 - -0.5 54,628 - -0.7 54,628 - +0.1 54,628 - +0.0 54,628 - +0.4
Or see this region's nucleotide sequence