Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3708 and GFF3709 are separated by 8 nucleotides GFF3709 and GFF3710 are separated by 58 nucleotides GFF3710 and GFF3711 are separated by 14 nucleotides
GFF3708 - ABC transporter, ATP-binding protein (cluster 11, riboflavin/purine nucleoside/unknown) / ABC transporter, ATP-binding protein (cluster 11, riboflavin/purine nucleoside/unknown), at 207,827 to 209,353
GFF3708
GFF3709 - ABC transporter, substrate-binding protein (cluster 11, riboflavin/purine nucleoside/unknown), at 209,362 to 210,471
GFF3709
GFF3710 - Lactoylglutathione lyase and related lyases, at 210,530 to 210,916
GFF3710
GFF3711 - oxidoreductase, 2OG-Fe(II) oxygenase family, at 210,931 to 211,932
GFF3711
Position (kb) 209
210
211 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 208.709 kb on + strand, within GFF3708 at 208.728 kb on + strand, within GFF3708 at 208.728 kb on + strand, within GFF3708 at 208.995 kb on - strand, within GFF3708 at 209.193 kb on + strand, within GFF3708 at 209.363 kb on + strand at 209.364 kb on - strand at 209.364 kb on - strand at 209.364 kb on - strand at 209.364 kb on - strand at 209.364 kb on - strand at 209.364 kb on - strand at 209.480 kb on + strand, within GFF3709 at 209.741 kb on + strand, within GFF3709 at 209.741 kb on + strand, within GFF3709 at 209.742 kb on - strand, within GFF3709 at 209.873 kb on + strand, within GFF3709 at 209.873 kb on + strand, within GFF3709 at 209.874 kb on - strand, within GFF3709 at 209.874 kb on - strand, within GFF3709 at 210.023 kb on + strand, within GFF3709 at 210.023 kb on + strand, within GFF3709 at 210.024 kb on - strand, within GFF3709 at 210.047 kb on + strand, within GFF3709 at 210.110 kb on + strand, within GFF3709 at 210.332 kb on + strand, within GFF3709 at 210.500 kb on + strand at 210.501 kb on - strand at 210.531 kb on + strand at 210.532 kb on - strand at 210.597 kb on + strand, within GFF3710 at 210.597 kb on + strand, within GFF3710 at 210.597 kb on + strand, within GFF3710 at 210.597 kb on + strand, within GFF3710 at 210.598 kb on - strand, within GFF3710 at 210.598 kb on - strand, within GFF3710 at 210.598 kb on - strand, within GFF3710 at 210.598 kb on - strand, within GFF3710 at 210.601 kb on - strand, within GFF3710 at 210.610 kb on - strand, within GFF3710 at 210.750 kb on + strand, within GFF3710 at 210.750 kb on + strand, within GFF3710 at 210.751 kb on - strand, within GFF3710 at 210.900 kb on + strand at 210.901 kb on - strand at 210.901 kb on - strand at 210.901 kb on - strand at 211.014 kb on - strand at 211.070 kb on + strand, within GFF3711 at 211.070 kb on + strand, within GFF3711
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 208,709 + GFF3708 0.58 -0.5 208,728 + GFF3708 0.59 +0.1 208,728 + GFF3708 0.59 -0.0 208,995 - GFF3708 0.76 -0.1 209,193 + GFF3708 0.89 -0.6 209,363 + -0.8 209,364 - -1.3 209,364 - +0.4 209,364 - +0.7 209,364 - -0.9 209,364 - -0.9 209,364 - -0.4 209,480 + GFF3709 0.11 +1.1 209,741 + GFF3709 0.34 -0.7 209,741 + GFF3709 0.34 -0.3 209,742 - GFF3709 0.34 +1.0 209,873 + GFF3709 0.46 -0.8 209,873 + GFF3709 0.46 -1.5 209,874 - GFF3709 0.46 -0.5 209,874 - GFF3709 0.46 +0.9 210,023 + GFF3709 0.60 +0.7 210,023 + GFF3709 0.60 +0.1 210,024 - GFF3709 0.60 -0.7 210,047 + GFF3709 0.62 +0.3 210,110 + GFF3709 0.67 +1.0 210,332 + GFF3709 0.87 -0.1 210,500 + +0.4 210,501 - +0.2 210,531 + -1.0 210,532 - +0.0 210,597 + GFF3710 0.17 +0.6 210,597 + GFF3710 0.17 -0.2 210,597 + GFF3710 0.17 -0.7 210,597 + GFF3710 0.17 +4.1 210,598 - GFF3710 0.18 -0.2 210,598 - GFF3710 0.18 -0.6 210,598 - GFF3710 0.18 -0.6 210,598 - GFF3710 0.18 -0.8 210,601 - GFF3710 0.18 -0.3 210,610 - GFF3710 0.21 -1.5 210,750 + GFF3710 0.57 +0.4 210,750 + GFF3710 0.57 +1.2 210,751 - GFF3710 0.57 +2.1 210,900 + -1.5 210,901 - +1.8 210,901 - -0.6 210,901 - +0.6 211,014 - -0.9 211,070 + GFF3711 0.14 +0.4 211,070 + GFF3711 0.14 +1.6
Or see this region's nucleotide sequence