Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3439 and GFF3440 are separated by 60 nucleotides GFF3440 and GFF3441 are separated by 5 nucleotides GFF3441 and GFF3442 are separated by 86 nucleotides GFF3442 and GFF3443 are separated by 6 nucleotides
GFF3439 - L-serine dehydratase, beta subunit (EC 4.3.1.17) / L-serine dehydratase, alpha subunit (EC 4.3.1.17), at 262,230 to 263,612
GFF3439
GFF3440 - FIG00460300: hypothetical protein, at 263,673 to 264,503
GFF3440
GFF3441 - Hypothetical protein VC0266 (sugar utilization related?), at 264,509 to 265,087
GFF3441
GFF3442 - RidA/YER057c/UK114 superfamily protein, at 265,174 to 265,578
GFF3442
GFF3443 - hypothetical protein, at 265,585 to 267,348
GFF3443
Position (kb)
264
265
266 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 263.562 kb on + strand at 263.562 kb on + strand at 263.562 kb on + strand at 263.562 kb on + strand at 263.562 kb on + strand at 263.562 kb on + strand at 263.563 kb on - strand at 263.563 kb on - strand at 263.563 kb on - strand at 263.647 kb on + strand at 263.648 kb on - strand at 263.648 kb on - strand at 263.838 kb on + strand, within GFF3440 at 263.839 kb on - strand, within GFF3440 at 263.865 kb on + strand, within GFF3440 at 263.865 kb on + strand, within GFF3440 at 263.865 kb on + strand, within GFF3440 at 263.866 kb on - strand, within GFF3440 at 263.866 kb on - strand, within GFF3440 at 263.866 kb on - strand, within GFF3440 at 263.866 kb on - strand, within GFF3440 at 263.866 kb on - strand, within GFF3440 at 263.866 kb on - strand, within GFF3440 at 264.192 kb on + strand, within GFF3440 at 264.192 kb on + strand, within GFF3440 at 264.193 kb on - strand, within GFF3440 at 264.219 kb on + strand, within GFF3440 at 264.219 kb on + strand, within GFF3440 at 264.220 kb on - strand, within GFF3440 at 264.722 kb on + strand, within GFF3441 at 264.723 kb on - strand, within GFF3441 at 264.723 kb on - strand, within GFF3441 at 264.723 kb on - strand, within GFF3441 at 264.723 kb on - strand, within GFF3441 at 264.723 kb on - strand, within GFF3441 at 264.758 kb on + strand, within GFF3441 at 264.759 kb on - strand, within GFF3441 at 264.759 kb on - strand, within GFF3441 at 264.873 kb on + strand, within GFF3441 at 264.873 kb on + strand, within GFF3441 at 264.874 kb on - strand, within GFF3441 at 264.874 kb on - strand, within GFF3441 at 264.882 kb on - strand, within GFF3441 at 264.954 kb on - strand, within GFF3441 at 265.097 kb on + strand at 265.098 kb on - strand at 265.098 kb on - strand at 265.098 kb on - strand at 265.270 kb on + strand, within GFF3442 at 265.270 kb on + strand, within GFF3442 at 265.270 kb on + strand, within GFF3442 at 265.270 kb on + strand, within GFF3442 at 265.270 kb on + strand, within GFF3442 at 265.270 kb on + strand, within GFF3442 at 265.270 kb on + strand, within GFF3442 at 265.270 kb on + strand, within GFF3442 at 265.270 kb on + strand, within GFF3442 at 265.270 kb on + strand, within GFF3442 at 265.271 kb on - strand, within GFF3442 at 265.271 kb on - strand, within GFF3442 at 265.271 kb on - strand, within GFF3442 at 265.271 kb on - strand, within GFF3442 at 265.271 kb on - strand, within GFF3442 at 265.271 kb on - strand, within GFF3442 at 265.271 kb on - strand, within GFF3442 at 265.271 kb on - strand, within GFF3442 at 265.271 kb on - strand, within GFF3442 at 265.423 kb on + strand, within GFF3442 at 265.423 kb on + strand, within GFF3442 at 265.424 kb on - strand, within GFF3442 at 265.424 kb on - strand, within GFF3442 at 265.424 kb on - strand, within GFF3442 at 265.424 kb on - strand, within GFF3442 at 265.424 kb on - strand, within GFF3442 at 265.465 kb on + strand, within GFF3442 at 265.465 kb on + strand, within GFF3442 at 265.465 kb on + strand, within GFF3442 at 265.465 kb on + strand, within GFF3442 at 265.465 kb on + strand, within GFF3442 at 265.465 kb on + strand, within GFF3442 at 265.465 kb on + strand, within GFF3442 at 265.465 kb on + strand, within GFF3442 at 265.465 kb on + strand, within GFF3442 at 265.465 kb on + strand, within GFF3442 at 265.466 kb on - strand, within GFF3442 at 265.466 kb on - strand, within GFF3442 at 265.466 kb on - strand, within GFF3442 at 265.466 kb on - strand, within GFF3442 at 265.466 kb on - strand, within GFF3442 at 265.637 kb on + strand at 265.637 kb on + strand at 265.638 kb on - strand at 265.638 kb on - strand at 265.638 kb on - strand at 265.638 kb on - strand at 265.638 kb on - strand at 265.806 kb on - strand, within GFF3443 at 265.913 kb on + strand, within GFF3443 at 265.913 kb on + strand, within GFF3443 at 265.914 kb on - strand, within GFF3443 at 265.914 kb on - strand, within GFF3443 at 265.914 kb on - strand, within GFF3443 at 265.914 kb on - strand, within GFF3443 at 265.914 kb on - strand, within GFF3443 at 265.914 kb on - strand, within GFF3443 at 265.914 kb on - strand, within GFF3443 at 266.039 kb on + strand, within GFF3443 at 266.039 kb on + strand, within GFF3443 at 266.039 kb on + strand, within GFF3443 at 266.040 kb on - strand, within GFF3443 at 266.040 kb on - strand, within GFF3443 at 266.040 kb on - strand, within GFF3443
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 263,562 + -0.1 263,562 + -0.6 263,562 + -0.2 263,562 + -0.2 263,562 + -1.3 263,562 + +0.4 263,563 - -0.0 263,563 - +0.5 263,563 - +0.4 263,647 + -0.9 263,648 - -1.9 263,648 - -0.3 263,838 + GFF3440 0.20 -0.1 263,839 - GFF3440 0.20 -0.2 263,865 + GFF3440 0.23 -1.3 263,865 + GFF3440 0.23 -1.3 263,865 + GFF3440 0.23 +0.4 263,866 - GFF3440 0.23 -0.5 263,866 - GFF3440 0.23 -0.3 263,866 - GFF3440 0.23 +0.0 263,866 - GFF3440 0.23 -0.9 263,866 - GFF3440 0.23 +0.7 263,866 - GFF3440 0.23 +0.0 264,192 + GFF3440 0.62 -0.2 264,192 + GFF3440 0.62 -0.7 264,193 - GFF3440 0.63 -2.3 264,219 + GFF3440 0.66 +0.1 264,219 + GFF3440 0.66 +1.8 264,220 - GFF3440 0.66 -0.5 264,722 + GFF3441 0.37 +0.0 264,723 - GFF3441 0.37 -0.2 264,723 - GFF3441 0.37 -0.8 264,723 - GFF3441 0.37 -1.3 264,723 - GFF3441 0.37 -1.2 264,723 - GFF3441 0.37 -1.4 264,758 + GFF3441 0.43 -0.5 264,759 - GFF3441 0.43 -1.3 264,759 - GFF3441 0.43 -0.2 264,873 + GFF3441 0.63 -1.5 264,873 + GFF3441 0.63 -0.4 264,874 - GFF3441 0.63 -0.6 264,874 - GFF3441 0.63 -1.9 264,882 - GFF3441 0.64 +0.8 264,954 - GFF3441 0.77 +1.1 265,097 + +3.6 265,098 - -0.0 265,098 - -0.8 265,098 - -0.2 265,270 + GFF3442 0.24 -1.0 265,270 + GFF3442 0.24 -0.7 265,270 + GFF3442 0.24 -0.2 265,270 + GFF3442 0.24 -0.2 265,270 + GFF3442 0.24 +1.2 265,270 + GFF3442 0.24 -0.8 265,270 + GFF3442 0.24 +1.8 265,270 + GFF3442 0.24 -0.1 265,270 + GFF3442 0.24 -0.2 265,270 + GFF3442 0.24 -1.4 265,271 - GFF3442 0.24 +0.2 265,271 - GFF3442 0.24 -0.3 265,271 - GFF3442 0.24 +1.0 265,271 - GFF3442 0.24 -0.3 265,271 - GFF3442 0.24 -1.4 265,271 - GFF3442 0.24 -0.6 265,271 - GFF3442 0.24 +0.8 265,271 - GFF3442 0.24 -0.3 265,271 - GFF3442 0.24 +0.1 265,423 + GFF3442 0.61 +1.2 265,423 + GFF3442 0.61 -0.2 265,424 - GFF3442 0.62 +0.8 265,424 - GFF3442 0.62 -0.1 265,424 - GFF3442 0.62 +0.1 265,424 - GFF3442 0.62 -1.0 265,424 - GFF3442 0.62 -0.2 265,465 + GFF3442 0.72 +0.8 265,465 + GFF3442 0.72 -0.3 265,465 + GFF3442 0.72 +1.0 265,465 + GFF3442 0.72 -2.2 265,465 + GFF3442 0.72 -1.0 265,465 + GFF3442 0.72 -0.3 265,465 + GFF3442 0.72 +0.0 265,465 + GFF3442 0.72 -2.3 265,465 + GFF3442 0.72 -0.5 265,465 + GFF3442 0.72 -0.8 265,466 - GFF3442 0.72 +0.2 265,466 - GFF3442 0.72 +0.2 265,466 - GFF3442 0.72 +1.1 265,466 - GFF3442 0.72 -0.7 265,466 - GFF3442 0.72 -0.6 265,637 + -0.9 265,637 + +1.6 265,638 - -0.2 265,638 - -1.0 265,638 - +0.0 265,638 - -0.8 265,638 - +0.6 265,806 - GFF3443 0.13 +0.3 265,913 + GFF3443 0.19 +1.0 265,913 + GFF3443 0.19 -0.8 265,914 - GFF3443 0.19 +0.8 265,914 - GFF3443 0.19 +3.2 265,914 - GFF3443 0.19 -0.0 265,914 - GFF3443 0.19 -1.4 265,914 - GFF3443 0.19 +0.1 265,914 - GFF3443 0.19 +0.1 265,914 - GFF3443 0.19 -0.3 266,039 + GFF3443 0.26 +0.2 266,039 + GFF3443 0.26 +0.4 266,039 + GFF3443 0.26 +0.6 266,040 - GFF3443 0.26 -0.4 266,040 - GFF3443 0.26 -0.4 266,040 - GFF3443 0.26 -0.2
Or see this region's nucleotide sequence