Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3424 and GFF3425 are separated by 104 nucleotides GFF3425 and GFF3426 overlap by 4 nucleotides GFF3426 and GFF3427 overlap by 4 nucleotides
GFF3424 - FIG00443816: hypothetical protein, at 248,999 to 249,709
GFF3424
GFF3425 - no description, at 249,814 to 250,161
GFF3425
GFF3426 - Predicted kinase, at 250,158 to 250,973
GFF3426
GFF3427 - FIG01006093: hypothetical protein, at 250,970 to 251,479
GFF3427
Position (kb) 249
250
251 Strain fitness (log2 ratio) -2
-1
0
1 at 249.031 kb on + strand at 249.032 kb on - strand at 249.032 kb on - strand at 249.084 kb on + strand, within GFF3424 at 249.085 kb on - strand, within GFF3424 at 249.303 kb on + strand, within GFF3424 at 249.303 kb on + strand, within GFF3424 at 249.303 kb on + strand, within GFF3424 at 249.304 kb on - strand, within GFF3424 at 249.304 kb on - strand, within GFF3424 at 249.304 kb on - strand, within GFF3424 at 249.319 kb on - strand, within GFF3424 at 249.375 kb on + strand, within GFF3424 at 249.376 kb on - strand, within GFF3424 at 249.456 kb on + strand, within GFF3424 at 249.456 kb on + strand, within GFF3424 at 249.456 kb on + strand, within GFF3424 at 249.457 kb on - strand, within GFF3424 at 249.507 kb on + strand, within GFF3424 at 249.507 kb on + strand, within GFF3424 at 249.507 kb on + strand, within GFF3424 at 249.508 kb on - strand, within GFF3424 at 249.508 kb on - strand, within GFF3424 at 249.513 kb on + strand, within GFF3424 at 249.579 kb on + strand, within GFF3424 at 249.579 kb on + strand, within GFF3424 at 249.579 kb on + strand, within GFF3424 at 249.579 kb on + strand, within GFF3424 at 249.579 kb on + strand, within GFF3424 at 249.579 kb on + strand, within GFF3424 at 249.579 kb on + strand, within GFF3424 at 249.579 kb on + strand, within GFF3424 at 249.580 kb on - strand, within GFF3424 at 249.580 kb on - strand, within GFF3424 at 249.580 kb on - strand, within GFF3424 at 249.580 kb on - strand, within GFF3424 at 249.580 kb on - strand, within GFF3424 at 249.580 kb on - strand, within GFF3424 at 250.057 kb on + strand, within GFF3425 at 250.057 kb on + strand, within GFF3425 at 250.058 kb on - strand, within GFF3425 at 250.269 kb on + strand, within GFF3426 at 250.269 kb on + strand, within GFF3426 at 250.569 kb on + strand, within GFF3426 at 250.569 kb on + strand, within GFF3426
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 249,031 + -1.0 249,032 - -0.6 249,032 - +0.3 249,084 + GFF3424 0.12 -1.0 249,085 - GFF3424 0.12 +0.6 249,303 + GFF3424 0.43 -0.6 249,303 + GFF3424 0.43 +1.7 249,303 + GFF3424 0.43 -0.2 249,304 - GFF3424 0.43 -1.4 249,304 - GFF3424 0.43 -0.1 249,304 - GFF3424 0.43 +0.2 249,319 - GFF3424 0.45 -0.2 249,375 + GFF3424 0.53 -1.1 249,376 - GFF3424 0.53 -0.1 249,456 + GFF3424 0.64 -0.2 249,456 + GFF3424 0.64 +1.0 249,456 + GFF3424 0.64 +0.2 249,457 - GFF3424 0.64 -0.6 249,507 + GFF3424 0.71 -0.6 249,507 + GFF3424 0.71 -0.5 249,507 + GFF3424 0.71 +0.2 249,508 - GFF3424 0.72 -0.2 249,508 - GFF3424 0.72 +0.2 249,513 + GFF3424 0.72 -2.0 249,579 + GFF3424 0.82 -0.2 249,579 + GFF3424 0.82 -0.4 249,579 + GFF3424 0.82 +0.5 249,579 + GFF3424 0.82 +0.7 249,579 + GFF3424 0.82 +0.9 249,579 + GFF3424 0.82 -0.0 249,579 + GFF3424 0.82 +0.4 249,579 + GFF3424 0.82 -0.8 249,580 - GFF3424 0.82 +0.0 249,580 - GFF3424 0.82 -0.1 249,580 - GFF3424 0.82 -0.0 249,580 - GFF3424 0.82 -0.6 249,580 - GFF3424 0.82 -0.7 249,580 - GFF3424 0.82 +1.6 250,057 + GFF3425 0.70 -2.0 250,057 + GFF3425 0.70 -0.8 250,058 - GFF3425 0.70 -0.2 250,269 + GFF3426 0.14 -1.5 250,269 + GFF3426 0.14 -1.5 250,569 + GFF3426 0.50 -0.9 250,569 + GFF3426 0.50 +0.1
Or see this region's nucleotide sequence