Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3263 and GFF3264 are separated by 562 nucleotides GFF3264 and GFF3265 overlap by 4 nucleotides
GFF3263 - Potassium voltage-gated channel subfamily KQT; possible potassium channel, VIC family, at 83,146 to 84,066
GFF3263
GFF3264 - Methionine biosynthesis protein MetW, at 84,629 to 85,219
GFF3264
GFF3265 - Homoserine O-acetyltransferase (EC 2.3.1.31), at 85,216 to 86,340
GFF3265
Position (kb)
84
85
86 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.710 kb on + strand, within GFF3263 at 83.711 kb on - strand, within GFF3263 at 83.711 kb on - strand, within GFF3263 at 83.711 kb on - strand, within GFF3263 at 83.711 kb on - strand, within GFF3263 at 83.711 kb on - strand, within GFF3263 at 83.711 kb on - strand, within GFF3263 at 83.711 kb on - strand, within GFF3263 at 83.711 kb on - strand, within GFF3263 at 83.711 kb on - strand, within GFF3263 at 83.761 kb on + strand, within GFF3263 at 83.762 kb on - strand, within GFF3263 at 83.762 kb on - strand, within GFF3263 at 84.270 kb on + strand at 84.271 kb on - strand at 84.271 kb on - strand at 84.271 kb on - strand at 84.271 kb on - strand at 84.271 kb on - strand at 84.271 kb on - strand at 84.271 kb on - strand at 84.315 kb on + strand at 84.315 kb on + strand at 84.316 kb on - strand at 84.316 kb on - strand at 84.801 kb on + strand, within GFF3264 at 84.801 kb on + strand, within GFF3264 at 84.801 kb on + strand, within GFF3264 at 84.801 kb on + strand, within GFF3264 at 84.801 kb on + strand, within GFF3264 at 84.802 kb on - strand, within GFF3264 at 84.802 kb on - strand, within GFF3264 at 84.802 kb on - strand, within GFF3264 at 84.802 kb on - strand, within GFF3264 at 84.802 kb on - strand, within GFF3264 at 84.811 kb on - strand, within GFF3264 at 85.271 kb on + strand at 85.486 kb on + strand, within GFF3265 at 85.491 kb on - strand, within GFF3265 at 85.610 kb on + strand, within GFF3265 at 85.691 kb on + strand, within GFF3265 at 85.691 kb on + strand, within GFF3265 at 85.754 kb on + strand, within GFF3265 at 86.028 kb on - strand, within GFF3265
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 83,710 + GFF3263 0.61 -0.1 83,710 + GFF3263 0.61 -0.4 83,710 + GFF3263 0.61 -0.3 83,710 + GFF3263 0.61 -0.2 83,710 + GFF3263 0.61 +0.2 83,710 + GFF3263 0.61 -0.7 83,710 + GFF3263 0.61 -0.4 83,710 + GFF3263 0.61 -0.9 83,710 + GFF3263 0.61 +0.7 83,710 + GFF3263 0.61 +0.3 83,710 + GFF3263 0.61 -0.7 83,710 + GFF3263 0.61 -1.0 83,710 + GFF3263 0.61 +0.0 83,710 + GFF3263 0.61 +0.0 83,711 - GFF3263 0.61 -0.2 83,711 - GFF3263 0.61 -0.4 83,711 - GFF3263 0.61 +0.2 83,711 - GFF3263 0.61 +1.4 83,711 - GFF3263 0.61 -1.0 83,711 - GFF3263 0.61 -0.7 83,711 - GFF3263 0.61 -0.1 83,711 - GFF3263 0.61 -0.1 83,711 - GFF3263 0.61 -0.6 83,761 + GFF3263 0.67 -1.5 83,762 - GFF3263 0.67 +0.1 83,762 - GFF3263 0.67 -0.4 84,270 + +0.2 84,271 - +1.4 84,271 - -1.0 84,271 - +0.4 84,271 - +0.2 84,271 - -0.4 84,271 - -0.5 84,271 - -0.4 84,315 + +0.1 84,315 + +0.8 84,316 - +0.8 84,316 - +0.0 84,801 + GFF3264 0.29 -1.3 84,801 + GFF3264 0.29 -0.8 84,801 + GFF3264 0.29 +1.2 84,801 + GFF3264 0.29 -2.3 84,801 + GFF3264 0.29 +0.2 84,802 - GFF3264 0.29 -0.5 84,802 - GFF3264 0.29 -1.4 84,802 - GFF3264 0.29 -1.4 84,802 - GFF3264 0.29 -2.3 84,802 - GFF3264 0.29 -2.5 84,811 - GFF3264 0.31 -2.0 85,271 + -0.2 85,486 + GFF3265 0.24 -1.5 85,491 - GFF3265 0.24 -3.0 85,610 + GFF3265 0.35 -1.3 85,691 + GFF3265 0.42 -1.3 85,691 + GFF3265 0.42 +0.2 85,754 + GFF3265 0.48 -1.0 86,028 - GFF3265 0.72 -1.6
Or see this region's nucleotide sequence