Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3228 and GFF3229 are separated by 63 nucleotides GFF3229 and GFF3230 are separated by 100 nucleotides GFF3230 and GFF3231 overlap by 4 nucleotides GFF3231 and GFF3232 overlap by 4 nucleotides
GFF3228 - hypothetical protein, at 48,736 to 49,665
GFF3228
GFF3229 - hypothetical protein, at 49,729 to 50,208
GFF3229
GFF3230 - no description, at 50,309 to 50,578
GFF3230
GFF3231 - hypothetical protein, at 50,575 to 51,186
GFF3231
GFF3232 - Phage lysozyme R (EC 3.2.1.17), at 51,183 to 51,758
GFF3232
Position (kb) 49
50
51 Strain fitness (log2 ratio) -2
-1
0
1
2 at 49.154 kb on + strand, within GFF3228 at 49.280 kb on + strand, within GFF3228 at 49.469 kb on + strand, within GFF3228 at 49.505 kb on + strand, within GFF3228 at 49.506 kb on - strand, within GFF3228 at 49.506 kb on - strand, within GFF3228 at 50.081 kb on + strand, within GFF3229 at 50.081 kb on + strand, within GFF3229 at 50.082 kb on - strand, within GFF3229 at 50.082 kb on - strand, within GFF3229 at 50.082 kb on - strand, within GFF3229 at 50.242 kb on + strand at 50.242 kb on + strand at 50.749 kb on + strand, within GFF3231 at 50.948 kb on + strand, within GFF3231 at 50.948 kb on + strand, within GFF3231 at 50.948 kb on + strand, within GFF3231 at 50.948 kb on + strand, within GFF3231 at 50.949 kb on + strand, within GFF3231 at 50.949 kb on - strand, within GFF3231 at 50.949 kb on - strand, within GFF3231 at 50.949 kb on - strand, within GFF3231 at 51.011 kb on + strand, within GFF3231 at 51.011 kb on + strand, within GFF3231
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 49,154 + GFF3228 0.45 +0.2 49,280 + GFF3228 0.58 -0.3 49,469 + GFF3228 0.79 +0.1 49,505 + GFF3228 0.83 -0.4 49,506 - GFF3228 0.83 +0.5 49,506 - GFF3228 0.83 +0.5 50,081 + GFF3229 0.73 -0.8 50,081 + GFF3229 0.73 -0.6 50,082 - GFF3229 0.74 -1.0 50,082 - GFF3229 0.74 -1.5 50,082 - GFF3229 0.74 +1.8 50,242 + -0.2 50,242 + +0.0 50,749 + GFF3231 0.28 -1.2 50,948 + GFF3231 0.61 -0.4 50,948 + GFF3231 0.61 +0.3 50,948 + GFF3231 0.61 -1.0 50,948 + GFF3231 0.61 -0.8 50,949 + GFF3231 0.61 -0.0 50,949 - GFF3231 0.61 +0.8 50,949 - GFF3231 0.61 -1.1 50,949 - GFF3231 0.61 -2.3 51,011 + GFF3231 0.71 -0.2 51,011 + GFF3231 0.71 -0.6
Or see this region's nucleotide sequence