Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3152 and GFF3153 overlap by 4 nucleotides GFF3153 and GFF3154 are separated by 188 nucleotides GFF3154 and GFF3155 are separated by 77 nucleotides
GFF3152 - hypothetical protein, at 587,719 to 589,104
GFF3152
GFF3153 - Two-component transcriptional response regulator, LuxR family, at 589,101 to 589,757
GFF3153
GFF3154 - no description, at 589,946 to 591,103
GFF3154
GFF3155 - Gluconolactonase (EC 3.1.1.17), at 591,181 to 592,110
GFF3155
Position (kb) 589
590
591
592 Strain fitness (log2 ratio) -2
-1
0
1 at 589.007 kb on + strand at 589.007 kb on + strand at 589.007 kb on + strand at 589.008 kb on - strand at 589.009 kb on - strand at 589.013 kb on + strand at 589.014 kb on - strand at 589.117 kb on + strand at 589.117 kb on + strand at 589.118 kb on - strand at 589.118 kb on - strand at 589.143 kb on - strand at 589.402 kb on + strand, within GFF3153 at 589.403 kb on - strand, within GFF3153 at 589.523 kb on + strand, within GFF3153 at 589.524 kb on - strand, within GFF3153 at 589.782 kb on + strand at 589.782 kb on + strand at 589.783 kb on - strand at 589.783 kb on - strand at 589.783 kb on - strand at 589.845 kb on + strand at 590.432 kb on + strand, within GFF3154 at 590.433 kb on - strand, within GFF3154 at 590.433 kb on - strand, within GFF3154 at 590.648 kb on + strand, within GFF3154 at 590.648 kb on + strand, within GFF3154 at 590.649 kb on - strand, within GFF3154 at 590.649 kb on - strand, within GFF3154 at 590.649 kb on - strand, within GFF3154 at 590.649 kb on - strand, within GFF3154 at 590.714 kb on + strand, within GFF3154 at 591.373 kb on + strand, within GFF3155 at 591.373 kb on + strand, within GFF3155 at 591.373 kb on + strand, within GFF3155 at 591.374 kb on - strand, within GFF3155 at 591.452 kb on - strand, within GFF3155 at 591.452 kb on - strand, within GFF3155 at 591.508 kb on + strand, within GFF3155 at 591.509 kb on - strand, within GFF3155 at 591.577 kb on + strand, within GFF3155 at 591.578 kb on - strand, within GFF3155 at 591.634 kb on + strand, within GFF3155 at 591.634 kb on + strand, within GFF3155 at 591.728 kb on - strand, within GFF3155 at 592.027 kb on + strand at 592.028 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 589,007 + -1.4 589,007 + -0.7 589,007 + +1.2 589,008 - +0.2 589,009 - -0.4 589,013 + -2.4 589,014 - -1.4 589,117 + +0.6 589,117 + +1.1 589,118 - -1.2 589,118 - +0.6 589,143 - -0.3 589,402 + GFF3153 0.46 -0.8 589,403 - GFF3153 0.46 +0.3 589,523 + GFF3153 0.64 -0.7 589,524 - GFF3153 0.64 -2.2 589,782 + -0.5 589,782 + +0.9 589,783 - -1.1 589,783 - -0.4 589,783 - +0.3 589,845 + -0.7 590,432 + GFF3154 0.42 +0.3 590,433 - GFF3154 0.42 -0.5 590,433 - GFF3154 0.42 -0.6 590,648 + GFF3154 0.61 -1.4 590,648 + GFF3154 0.61 -1.2 590,649 - GFF3154 0.61 +0.4 590,649 - GFF3154 0.61 -0.9 590,649 - GFF3154 0.61 -2.0 590,649 - GFF3154 0.61 -0.7 590,714 + GFF3154 0.66 +0.4 591,373 + GFF3155 0.21 -1.1 591,373 + GFF3155 0.21 -0.3 591,373 + GFF3155 0.21 -0.6 591,374 - GFF3155 0.21 -0.8 591,452 - GFF3155 0.29 +0.8 591,452 - GFF3155 0.29 +0.0 591,508 + GFF3155 0.35 -1.1 591,509 - GFF3155 0.35 +1.2 591,577 + GFF3155 0.43 -1.3 591,578 - GFF3155 0.43 -0.5 591,634 + GFF3155 0.49 -1.1 591,634 + GFF3155 0.49 -1.3 591,728 - GFF3155 0.59 +0.1 592,027 + +0.2 592,028 - -0.3
Or see this region's nucleotide sequence