Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3144 and GFF3145 are separated by 2 nucleotides GFF3145 and GFF3146 are separated by 43 nucleotides GFF3146 and GFF3147 are separated by 126 nucleotides
GFF3144 - hypothetical protein, at 577,866 to 579,218
GFF3144
GFF3145 - Two-component system response regulator QseB, at 579,221 to 579,847
GFF3145
GFF3146 - no description, at 579,891 to 580,481
GFF3146
GFF3147 - hypothetical protein, at 580,608 to 581,993
GFF3147
Position (kb) 579
580 Strain fitness (log2 ratio) -1
0
1 at 578.929 kb on + strand, within GFF3144 at 578.930 kb on - strand, within GFF3144 at 579.136 kb on + strand at 579.136 kb on + strand at 579.137 kb on - strand at 579.137 kb on - strand at 579.137 kb on - strand at 579.292 kb on - strand, within GFF3145 at 579.343 kb on - strand, within GFF3145 at 579.349 kb on - strand, within GFF3145 at 579.585 kb on + strand, within GFF3145 at 579.585 kb on + strand, within GFF3145 at 579.585 kb on + strand, within GFF3145 at 579.866 kb on + strand at 579.866 kb on + strand at 579.938 kb on - strand at 580.210 kb on + strand, within GFF3146 at 580.210 kb on + strand, within GFF3146 at 580.210 kb on + strand, within GFF3146 at 580.211 kb on - strand, within GFF3146 at 580.464 kb on + strand at 580.513 kb on - strand at 580.513 kb on - strand at 580.513 kb on - strand at 580.523 kb on + strand at 580.523 kb on + strand at 580.523 kb on + strand at 580.524 kb on - strand at 580.524 kb on - strand at 580.558 kb on - strand at 580.699 kb on - strand at 580.699 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 578,929 + GFF3144 0.79 +0.2 578,930 - GFF3144 0.79 +0.1 579,136 + -0.3 579,136 + -0.7 579,137 - -0.9 579,137 - -1.2 579,137 - -0.4 579,292 - GFF3145 0.11 +0.2 579,343 - GFF3145 0.19 -0.9 579,349 - GFF3145 0.20 +0.9 579,585 + GFF3145 0.58 -0.5 579,585 + GFF3145 0.58 +1.2 579,585 + GFF3145 0.58 -0.6 579,866 + -0.7 579,866 + +1.3 579,938 - +0.6 580,210 + GFF3146 0.54 +1.0 580,210 + GFF3146 0.54 -0.6 580,210 + GFF3146 0.54 +0.2 580,211 - GFF3146 0.54 +0.8 580,464 + +0.4 580,513 - +0.6 580,513 - -0.1 580,513 - +0.4 580,523 + -0.7 580,523 + -0.3 580,523 + -0.4 580,524 - -1.4 580,524 - -0.4 580,558 - +0.1 580,699 - +0.2 580,699 - -0.4
Or see this region's nucleotide sequence