Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF270 and GFF271 are separated by 70 nucleotides GFF271 and GFF272 overlap by 4 nucleotides GFF272 and GFF273 are separated by 108 nucleotides
GFF270 - 2-methylcitrate dehydratase (2-methyl-trans-aconitate forming) (EC 4.2.1.117), at 304,528 to 307,146
GFF270
GFF271 - 2-methylcitrate synthase (EC 2.3.3.5), at 307,217 to 308,380
GFF271
GFF272 - thioesterase superfamily protein, at 308,377 to 308,817
GFF272
GFF273 - Transcriptional regulator, Xre family, at 308,926 to 310,347
GFF273
Position (kb)
307
308
309 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 306.332 kb on + strand, within GFF270 at 306.332 kb on + strand, within GFF270 at 306.332 kb on + strand, within GFF270 at 306.332 kb on + strand, within GFF270 at 306.333 kb on - strand, within GFF270 at 307.040 kb on + strand at 307.040 kb on + strand at 307.041 kb on - strand at 307.041 kb on - strand at 307.041 kb on - strand at 307.041 kb on - strand at 307.041 kb on - strand at 307.041 kb on - strand at 307.041 kb on - strand at 307.042 kb on - strand at 307.100 kb on + strand at 307.100 kb on + strand at 307.100 kb on + strand at 307.100 kb on + strand at 307.101 kb on - strand at 307.101 kb on - strand at 307.101 kb on - strand at 307.101 kb on - strand at 307.140 kb on + strand at 307.141 kb on - strand at 307.218 kb on + strand at 307.266 kb on + strand at 307.266 kb on + strand at 307.266 kb on + strand at 307.266 kb on + strand at 307.266 kb on + strand at 307.267 kb on - strand at 307.267 kb on - strand at 307.267 kb on - strand at 307.267 kb on - strand at 307.267 kb on - strand at 307.267 kb on - strand at 307.267 kb on - strand at 307.316 kb on - strand at 307.392 kb on + strand, within GFF271 at 307.392 kb on + strand, within GFF271 at 307.392 kb on + strand, within GFF271 at 307.392 kb on + strand, within GFF271 at 307.392 kb on + strand, within GFF271 at 307.393 kb on - strand, within GFF271 at 307.393 kb on - strand, within GFF271 at 307.393 kb on - strand, within GFF271 at 307.482 kb on + strand, within GFF271 at 307.557 kb on + strand, within GFF271 at 307.557 kb on + strand, within GFF271 at 307.558 kb on - strand, within GFF271 at 307.558 kb on - strand, within GFF271 at 307.558 kb on - strand, within GFF271 at 307.558 kb on - strand, within GFF271 at 307.558 kb on - strand, within GFF271 at 307.642 kb on - strand, within GFF271 at 307.728 kb on + strand, within GFF271 at 307.728 kb on + strand, within GFF271 at 307.728 kb on + strand, within GFF271 at 307.728 kb on + strand, within GFF271 at 307.728 kb on + strand, within GFF271 at 307.728 kb on + strand, within GFF271 at 307.728 kb on + strand, within GFF271 at 307.728 kb on + strand, within GFF271 at 307.728 kb on + strand, within GFF271 at 307.728 kb on + strand, within GFF271 at 307.729 kb on - strand, within GFF271 at 307.729 kb on - strand, within GFF271 at 307.729 kb on - strand, within GFF271 at 307.729 kb on - strand, within GFF271 at 307.729 kb on - strand, within GFF271 at 307.729 kb on - strand, within GFF271 at 307.729 kb on - strand, within GFF271 at 307.729 kb on - strand, within GFF271 at 307.729 kb on - strand, within GFF271 at 307.729 kb on - strand, within GFF271 at 307.729 kb on - strand, within GFF271 at 307.729 kb on - strand, within GFF271 at 307.729 kb on - strand, within GFF271 at 307.729 kb on - strand, within GFF271 at 307.797 kb on + strand, within GFF271 at 307.798 kb on - strand, within GFF271 at 307.936 kb on - strand, within GFF271 at 307.936 kb on - strand, within GFF271 at 307.941 kb on + strand, within GFF271 at 307.941 kb on + strand, within GFF271 at 307.941 kb on + strand, within GFF271 at 307.941 kb on + strand, within GFF271 at 307.941 kb on + strand, within GFF271 at 307.942 kb on - strand, within GFF271 at 307.942 kb on - strand, within GFF271 at 307.942 kb on - strand, within GFF271 at 307.942 kb on - strand, within GFF271 at 307.942 kb on - strand, within GFF271 at 307.942 kb on - strand, within GFF271 at 307.942 kb on - strand, within GFF271 at 308.151 kb on + strand, within GFF271 at 308.151 kb on + strand, within GFF271 at 308.151 kb on + strand, within GFF271 at 308.151 kb on + strand, within GFF271 at 308.152 kb on - strand, within GFF271 at 308.152 kb on - strand, within GFF271 at 308.152 kb on - strand, within GFF271 at 308.152 kb on - strand, within GFF271 at 308.152 kb on - strand, within GFF271 at 308.152 kb on - strand, within GFF271 at 308.194 kb on + strand, within GFF271 at 308.257 kb on - strand, within GFF271 at 308.257 kb on - strand, within GFF271 at 308.414 kb on + strand at 308.415 kb on - strand at 308.415 kb on - strand at 308.723 kb on + strand, within GFF272 at 308.723 kb on + strand, within GFF272 at 308.822 kb on + strand at 309.025 kb on + strand at 309.025 kb on + strand at 309.025 kb on + strand at 309.025 kb on + strand at 309.026 kb on - strand at 309.026 kb on - strand at 309.026 kb on - strand at 309.026 kb on - strand at 309.026 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 306,332 + GFF270 0.69 -2.0 306,332 + GFF270 0.69 -3.0 306,332 + GFF270 0.69 -3.0 306,332 + GFF270 0.69 -1.1 306,333 - GFF270 0.69 -2.8 307,040 + -2.8 307,040 + -2.3 307,041 - -1.5 307,041 - -0.3 307,041 - -1.8 307,041 - -1.5 307,041 - -1.7 307,041 - -2.1 307,041 - -1.3 307,042 - -2.7 307,100 + -1.1 307,100 + -1.8 307,100 + -1.9 307,100 + -1.8 307,101 - -1.5 307,101 - -3.0 307,101 - -0.9 307,101 - -1.8 307,140 + +0.1 307,141 - -2.3 307,218 + +0.2 307,266 + -2.2 307,266 + -0.3 307,266 + -2.7 307,266 + -0.3 307,266 + -3.1 307,267 - -2.0 307,267 - -1.9 307,267 - -1.7 307,267 - -1.3 307,267 - -2.5 307,267 - -0.8 307,267 - -0.8 307,316 - -0.4 307,392 + GFF271 0.15 -1.9 307,392 + GFF271 0.15 +0.2 307,392 + GFF271 0.15 -0.4 307,392 + GFF271 0.15 -2.8 307,392 + GFF271 0.15 -1.5 307,393 - GFF271 0.15 -1.7 307,393 - GFF271 0.15 -0.3 307,393 - GFF271 0.15 -1.0 307,482 + GFF271 0.23 -0.9 307,557 + GFF271 0.29 -1.4 307,557 + GFF271 0.29 -1.2 307,558 - GFF271 0.29 +0.3 307,558 - GFF271 0.29 -2.1 307,558 - GFF271 0.29 -1.0 307,558 - GFF271 0.29 -0.7 307,558 - GFF271 0.29 -3.2 307,642 - GFF271 0.37 -2.6 307,728 + GFF271 0.44 -0.1 307,728 + GFF271 0.44 -1.5 307,728 + GFF271 0.44 -1.0 307,728 + GFF271 0.44 -1.5 307,728 + GFF271 0.44 -2.6 307,728 + GFF271 0.44 -2.2 307,728 + GFF271 0.44 +0.5 307,728 + GFF271 0.44 -1.1 307,728 + GFF271 0.44 -1.3 307,728 + GFF271 0.44 -2.3 307,729 - GFF271 0.44 -1.0 307,729 - GFF271 0.44 +0.0 307,729 - GFF271 0.44 -1.1 307,729 - GFF271 0.44 -0.8 307,729 - GFF271 0.44 +0.0 307,729 - GFF271 0.44 -0.3 307,729 - GFF271 0.44 +1.3 307,729 - GFF271 0.44 -1.0 307,729 - GFF271 0.44 -1.6 307,729 - GFF271 0.44 -2.2 307,729 - GFF271 0.44 -0.3 307,729 - GFF271 0.44 +0.7 307,729 - GFF271 0.44 +0.0 307,729 - GFF271 0.44 -2.0 307,797 + GFF271 0.50 -1.5 307,798 - GFF271 0.50 -1.3 307,936 - GFF271 0.62 -2.0 307,936 - GFF271 0.62 -1.3 307,941 + GFF271 0.62 -1.5 307,941 + GFF271 0.62 -1.3 307,941 + GFF271 0.62 -1.3 307,941 + GFF271 0.62 -1.5 307,941 + GFF271 0.62 +0.7 307,942 - GFF271 0.62 -1.8 307,942 - GFF271 0.62 +0.2 307,942 - GFF271 0.62 -0.7 307,942 - GFF271 0.62 +0.2 307,942 - GFF271 0.62 -0.7 307,942 - GFF271 0.62 -1.1 307,942 - GFF271 0.62 -0.7 308,151 + GFF271 0.80 -1.8 308,151 + GFF271 0.80 +1.3 308,151 + GFF271 0.80 -0.8 308,151 + GFF271 0.80 -1.5 308,152 - GFF271 0.80 -0.7 308,152 - GFF271 0.80 -2.6 308,152 - GFF271 0.80 -0.8 308,152 - GFF271 0.80 -0.4 308,152 - GFF271 0.80 -3.2 308,152 - GFF271 0.80 -1.5 308,194 + GFF271 0.84 -1.0 308,257 - GFF271 0.89 -0.6 308,257 - GFF271 0.89 -1.2 308,414 + -0.8 308,415 - -0.8 308,415 - -0.7 308,723 + GFF272 0.78 -1.4 308,723 + GFF272 0.78 -0.1 308,822 + -0.1 309,025 + -1.0 309,025 + -1.7 309,025 + +0.5 309,025 + -0.3 309,026 - -1.0 309,026 - -1.8 309,026 - -2.6 309,026 - -2.0 309,026 - -1.3
Or see this region's nucleotide sequence