Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2703 and GFF2704 are separated by 10 nucleotides GFF2704 and GFF2705 are separated by 6 nucleotides GFF2705 and GFF2706 are separated by 530 nucleotides
GFF2703 - Hydroxymethylpyrimidine ABC transporter, transmembrane component, at 131,005 to 131,868
GFF2703
GFF2704 - Hydroxymethylpyrimidine ABC transporter, ATP-binding protein, at 131,879 to 132,673
GFF2704
GFF2705 - Hydroxymethylpyrimidine ABC transporter, substrate-binding component, at 132,680 to 133,711
GFF2705
GFF2706 - VgrG protein, at 134,242 to 136,962
GFF2706
Position (kb) 132
133
134 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 132.749 kb on - strand at 132.784 kb on - strand, within GFF2705 at 132.861 kb on + strand, within GFF2705 at 132.861 kb on + strand, within GFF2705 at 133.041 kb on + strand, within GFF2705 at 133.045 kb on - strand, within GFF2705 at 133.335 kb on + strand, within GFF2705 at 133.341 kb on + strand, within GFF2705 at 133.341 kb on + strand, within GFF2705 at 133.342 kb on - strand, within GFF2705 at 133.342 kb on - strand, within GFF2705 at 133.521 kb on + strand, within GFF2705 at 133.522 kb on - strand, within GFF2705 at 133.812 kb on + strand at 133.870 kb on - strand at 133.945 kb on - strand at 134.049 kb on + strand at 134.049 kb on + strand at 134.165 kb on + strand at 134.166 kb on - strand at 134.299 kb on + strand at 134.408 kb on - strand at 134.660 kb on - strand, within GFF2706 at 134.698 kb on + strand, within GFF2706 at 134.698 kb on + strand, within GFF2706 at 134.699 kb on - strand, within GFF2706
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 132,749 - -0.1 132,784 - GFF2705 0.10 +1.2 132,861 + GFF2705 0.18 -0.6 132,861 + GFF2705 0.18 +0.1 133,041 + GFF2705 0.35 -0.0 133,045 - GFF2705 0.35 +1.2 133,335 + GFF2705 0.63 +0.4 133,341 + GFF2705 0.64 +0.1 133,341 + GFF2705 0.64 +0.0 133,342 - GFF2705 0.64 +0.8 133,342 - GFF2705 0.64 +1.4 133,521 + GFF2705 0.81 +0.8 133,522 - GFF2705 0.82 +1.4 133,812 + -0.2 133,870 - -1.1 133,945 - -0.3 134,049 + -0.6 134,049 + -1.7 134,165 + +0.1 134,166 - -0.2 134,299 + +0.2 134,408 - -0.3 134,660 - GFF2706 0.15 +0.0 134,698 + GFF2706 0.17 +0.2 134,698 + GFF2706 0.17 -1.4 134,699 - GFF2706 0.17 -2.8
Or see this region's nucleotide sequence