Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2697 and GFF2698 are separated by 49 nucleotides GFF2698 and GFF2699 are separated by 27 nucleotides GFF2699 and GFF2700 are separated by 28 nucleotides GFF2700 and GFF2701 overlap by 4 nucleotides
GFF2697 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 125,501 to 126,262
GFF2697
GFF2698 - Transcriptional regulator, LysR family, at 126,312 to 127,226
GFF2698
GFF2699 - 4Fe-4S ferredoxin, iron-sulfur binding, at 127,254 to 127,589
GFF2699
GFF2700 - Enoyl-CoA hydratase (EC 4.2.1.17), at 127,618 to 128,394
GFF2700
GFF2701 - CaiB/BaiF family protein, at 128,391 to 129,560
GFF2701
Position (kb) 127
128 Strain fitness (log2 ratio) -2
-1
0
1 at 126.862 kb on + strand, within GFF2698 at 126.862 kb on + strand, within GFF2698 at 126.862 kb on + strand, within GFF2698 at 126.863 kb on - strand, within GFF2698 at 126.863 kb on - strand, within GFF2698 at 127.204 kb on + strand at 127.423 kb on + strand, within GFF2699 at 127.489 kb on + strand, within GFF2699 at 127.511 kb on - strand, within GFF2699 at 127.511 kb on - strand, within GFF2699 at 127.597 kb on - strand at 127.620 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 126,862 + GFF2698 0.60 -2.0 126,862 + GFF2698 0.60 +0.8 126,862 + GFF2698 0.60 -0.5 126,863 - GFF2698 0.60 +0.3 126,863 - GFF2698 0.60 -0.1 127,204 + -0.9 127,423 + GFF2699 0.50 -1.0 127,489 + GFF2699 0.70 +0.0 127,511 - GFF2699 0.76 -0.8 127,511 - GFF2699 0.76 -1.3 127,597 - -0.4 127,620 - +0.1
Or see this region's nucleotide sequence