Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF253 and GFF254 are separated by 31 nucleotides GFF254 and GFF255 are separated by 40 nucleotides GFF255 and GFF256 are separated by 65 nucleotides
GFF253 - Transcriptional regulator, LysR family, at 284,828 to 285,727
GFF253
GFF254 - N-succinyl-L,L-diaminopimelate aminotransferase (EC 2.6.1.17), type 2, at 285,759 to 286,979
GFF254
GFF255 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117), at 287,020 to 287,853
GFF255
GFF256 - Twitching motility protein PilT, at 287,919 to 289,067
GFF256
Position (kb) 285
286
287 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 284.816 kb on - strand at 284.837 kb on + strand at 285.326 kb on + strand, within GFF253 at 285.326 kb on + strand, within GFF253 at 285.326 kb on + strand, within GFF253 at 285.326 kb on + strand, within GFF253 at 285.326 kb on + strand, within GFF253 at 285.326 kb on + strand, within GFF253 at 285.326 kb on + strand, within GFF253 at 285.326 kb on + strand, within GFF253 at 285.326 kb on + strand, within GFF253 at 285.326 kb on + strand, within GFF253 at 285.326 kb on + strand, within GFF253 at 285.326 kb on + strand, within GFF253 at 285.327 kb on - strand, within GFF253 at 285.327 kb on - strand, within GFF253 at 285.327 kb on - strand, within GFF253 at 285.327 kb on - strand, within GFF253 at 285.327 kb on - strand, within GFF253 at 285.327 kb on - strand, within GFF253 at 285.327 kb on - strand, within GFF253 at 285.327 kb on - strand, within GFF253 at 285.327 kb on - strand, within GFF253 at 285.327 kb on - strand, within GFF253 at 285.359 kb on + strand, within GFF253 at 285.359 kb on + strand, within GFF253 at 285.359 kb on + strand, within GFF253 at 285.359 kb on + strand, within GFF253 at 285.360 kb on - strand, within GFF253 at 285.360 kb on - strand, within GFF253 at 285.360 kb on - strand, within GFF253 at 285.360 kb on - strand, within GFF253 at 285.360 kb on - strand, within GFF253 at 285.369 kb on - strand, within GFF253 at 285.369 kb on - strand, within GFF253 at 285.369 kb on - strand, within GFF253 at 285.369 kb on - strand, within GFF253 at 285.369 kb on - strand, within GFF253 at 285.653 kb on + strand at 285.654 kb on - strand at 285.708 kb on + strand at 285.708 kb on + strand at 285.708 kb on + strand at 285.708 kb on + strand at 285.708 kb on + strand at 285.708 kb on + strand at 285.709 kb on - strand at 285.709 kb on - strand at 286.002 kb on + strand, within GFF254 at 286.002 kb on + strand, within GFF254 at 286.137 kb on + strand, within GFF254 at 286.137 kb on + strand, within GFF254 at 286.138 kb on - strand, within GFF254 at 286.138 kb on - strand, within GFF254 at 286.138 kb on - strand, within GFF254 at 286.147 kb on - strand, within GFF254 at 286.147 kb on - strand, within GFF254 at 286.147 kb on - strand, within GFF254 at 286.147 kb on - strand, within GFF254 at 286.147 kb on - strand, within GFF254 at 286.147 kb on - strand, within GFF254 at 286.182 kb on + strand, within GFF254 at 286.182 kb on + strand, within GFF254 at 286.182 kb on + strand, within GFF254 at 286.183 kb on - strand, within GFF254 at 286.185 kb on + strand, within GFF254 at 286.185 kb on + strand, within GFF254 at 286.186 kb on - strand, within GFF254 at 286.186 kb on - strand, within GFF254 at 286.186 kb on - strand, within GFF254 at 286.186 kb on - strand, within GFF254 at 286.380 kb on + strand, within GFF254 at 286.380 kb on + strand, within GFF254 at 286.380 kb on + strand, within GFF254 at 286.381 kb on - strand, within GFF254 at 286.381 kb on - strand, within GFF254 at 286.381 kb on - strand, within GFF254 at 286.393 kb on - strand, within GFF254 at 286.393 kb on - strand, within GFF254 at 286.393 kb on - strand, within GFF254 at 286.393 kb on - strand, within GFF254 at 286.393 kb on - strand, within GFF254 at 286.393 kb on - strand, within GFF254 at 286.525 kb on + strand, within GFF254 at 286.526 kb on - strand, within GFF254 at 286.564 kb on - strand, within GFF254 at 286.572 kb on + strand, within GFF254 at 286.572 kb on + strand, within GFF254 at 286.572 kb on + strand, within GFF254 at 286.572 kb on + strand, within GFF254 at 286.573 kb on - strand, within GFF254 at 286.573 kb on - strand, within GFF254 at 286.573 kb on - strand, within GFF254 at 286.573 kb on - strand, within GFF254 at 286.662 kb on + strand, within GFF254 at 286.663 kb on - strand, within GFF254 at 286.663 kb on - strand, within GFF254 at 286.663 kb on - strand, within GFF254 at 286.737 kb on + strand, within GFF254 at 286.737 kb on + strand, within GFF254 at 286.737 kb on + strand, within GFF254 at 286.737 kb on + strand, within GFF254 at 286.738 kb on - strand, within GFF254 at 286.753 kb on + strand, within GFF254 at 286.800 kb on + strand, within GFF254 at 286.800 kb on + strand, within GFF254 at 286.800 kb on + strand, within GFF254 at 286.801 kb on - strand, within GFF254 at 286.801 kb on - strand, within GFF254 at 286.809 kb on + strand, within GFF254 at 286.809 kb on + strand, within GFF254 at 286.809 kb on + strand, within GFF254 at 286.809 kb on + strand, within GFF254 at 286.809 kb on + strand, within GFF254 at 286.809 kb on + strand, within GFF254 at 286.809 kb on + strand, within GFF254 at 286.809 kb on + strand, within GFF254 at 286.810 kb on - strand, within GFF254 at 286.810 kb on - strand, within GFF254 at 286.810 kb on - strand, within GFF254 at 286.810 kb on - strand, within GFF254 at 286.810 kb on - strand, within GFF254 at 286.810 kb on - strand, within GFF254 at 286.810 kb on - strand, within GFF254 at 286.810 kb on - strand, within GFF254 at 286.810 kb on - strand, within GFF254 at 286.836 kb on + strand, within GFF254 at 286.836 kb on + strand, within GFF254 at 286.836 kb on + strand, within GFF254 at 286.836 kb on + strand, within GFF254 at 286.837 kb on - strand, within GFF254 at 286.837 kb on - strand, within GFF254 at 286.837 kb on - strand, within GFF254 at 286.837 kb on - strand, within GFF254 at 286.837 kb on - strand, within GFF254 at 287.423 kb on - strand, within GFF255 at 287.644 kb on + strand, within GFF255 at 287.644 kb on + strand, within GFF255 at 287.644 kb on + strand, within GFF255 at 287.644 kb on + strand, within GFF255 at 287.644 kb on + strand, within GFF255 at 287.693 kb on - strand, within GFF255 at 287.767 kb on + strand, within GFF255 at 287.776 kb on + strand at 287.776 kb on + strand at 287.777 kb on - strand at 287.972 kb on + strand at 287.972 kb on + strand at 287.972 kb on + strand at 287.973 kb on - strand at 287.973 kb on - strand at 287.973 kb on - strand at 287.976 kb on + strand at 287.976 kb on + strand at 287.976 kb on + strand at 287.976 kb on + strand at 287.976 kb on + strand at 287.976 kb on + strand at 287.976 kb on + strand at 287.976 kb on + strand at 287.976 kb on + strand at 287.976 kb on + strand at 287.976 kb on + strand at 287.977 kb on - strand at 287.977 kb on - strand at 287.977 kb on - strand at 287.977 kb on - strand at 287.977 kb on - strand at 287.977 kb on - strand at 287.977 kb on - strand at 287.977 kb on - strand at 287.977 kb on - strand at 287.977 kb on - strand at 287.977 kb on - strand at 287.977 kb on - strand at 287.977 kb on - strand at 287.977 kb on - strand at 287.977 kb on - strand at 287.977 kb on - strand at 287.977 kb on - strand at 287.977 kb on - strand at 287.977 kb on - strand at 287.977 kb on - strand at 287.977 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 284,816 - +0.6 284,837 + +0.3 285,326 + GFF253 0.55 -0.5 285,326 + GFF253 0.55 +0.9 285,326 + GFF253 0.55 -0.4 285,326 + GFF253 0.55 -1.0 285,326 + GFF253 0.55 +0.1 285,326 + GFF253 0.55 +0.6 285,326 + GFF253 0.55 -0.3 285,326 + GFF253 0.55 -0.7 285,326 + GFF253 0.55 -0.3 285,326 + GFF253 0.55 -1.7 285,326 + GFF253 0.55 -0.1 285,326 + GFF253 0.55 +1.4 285,327 - GFF253 0.55 -0.3 285,327 - GFF253 0.55 +1.3 285,327 - GFF253 0.55 -0.6 285,327 - GFF253 0.55 -0.4 285,327 - GFF253 0.55 +1.1 285,327 - GFF253 0.55 -0.4 285,327 - GFF253 0.55 -1.3 285,327 - GFF253 0.55 -0.5 285,327 - GFF253 0.55 +1.1 285,327 - GFF253 0.55 +0.2 285,359 + GFF253 0.59 -1.5 285,359 + GFF253 0.59 +0.3 285,359 + GFF253 0.59 -1.1 285,359 + GFF253 0.59 -0.6 285,360 - GFF253 0.59 -0.1 285,360 - GFF253 0.59 +0.7 285,360 - GFF253 0.59 +1.1 285,360 - GFF253 0.59 -0.2 285,360 - GFF253 0.59 +0.3 285,369 - GFF253 0.60 -0.3 285,369 - GFF253 0.60 +1.3 285,369 - GFF253 0.60 +0.1 285,369 - GFF253 0.60 -0.1 285,369 - GFF253 0.60 +1.2 285,653 + -1.0 285,654 - +0.3 285,708 + -0.1 285,708 + +0.7 285,708 + +0.2 285,708 + +0.7 285,708 + -0.1 285,708 + -1.5 285,709 - -0.3 285,709 - +0.3 286,002 + GFF254 0.20 -2.9 286,002 + GFF254 0.20 -0.7 286,137 + GFF254 0.31 -1.9 286,137 + GFF254 0.31 -1.0 286,138 - GFF254 0.31 -2.2 286,138 - GFF254 0.31 -0.7 286,138 - GFF254 0.31 +0.7 286,147 - GFF254 0.32 -0.3 286,147 - GFF254 0.32 -0.1 286,147 - GFF254 0.32 -0.3 286,147 - GFF254 0.32 +0.6 286,147 - GFF254 0.32 -2.5 286,147 - GFF254 0.32 -1.1 286,182 + GFF254 0.35 -0.3 286,182 + GFF254 0.35 -0.0 286,182 + GFF254 0.35 -1.7 286,183 - GFF254 0.35 -1.2 286,185 + GFF254 0.35 -2.4 286,185 + GFF254 0.35 -0.7 286,186 - GFF254 0.35 -1.1 286,186 - GFF254 0.35 +0.1 286,186 - GFF254 0.35 -0.5 286,186 - GFF254 0.35 -1.5 286,380 + GFF254 0.51 -1.0 286,380 + GFF254 0.51 -0.5 286,380 + GFF254 0.51 +1.0 286,381 - GFF254 0.51 -2.1 286,381 - GFF254 0.51 -1.1 286,381 - GFF254 0.51 -1.0 286,393 - GFF254 0.52 -0.4 286,393 - GFF254 0.52 -0.6 286,393 - GFF254 0.52 -0.3 286,393 - GFF254 0.52 -0.6 286,393 - GFF254 0.52 -0.7 286,393 - GFF254 0.52 -1.1 286,525 + GFF254 0.63 -1.3 286,526 - GFF254 0.63 +0.3 286,564 - GFF254 0.66 -1.9 286,572 + GFF254 0.67 +0.1 286,572 + GFF254 0.67 -1.5 286,572 + GFF254 0.67 -1.2 286,572 + GFF254 0.67 -0.5 286,573 - GFF254 0.67 -1.9 286,573 - GFF254 0.67 -0.3 286,573 - GFF254 0.67 -1.1 286,573 - GFF254 0.67 +0.7 286,662 + GFF254 0.74 -1.1 286,663 - GFF254 0.74 +1.7 286,663 - GFF254 0.74 -0.9 286,663 - GFF254 0.74 -1.3 286,737 + GFF254 0.80 +0.2 286,737 + GFF254 0.80 +0.3 286,737 + GFF254 0.80 -1.2 286,737 + GFF254 0.80 -1.7 286,738 - GFF254 0.80 -2.1 286,753 + GFF254 0.81 -0.9 286,800 + GFF254 0.85 -0.3 286,800 + GFF254 0.85 -2.4 286,800 + GFF254 0.85 -2.8 286,801 - GFF254 0.85 -2.7 286,801 - GFF254 0.85 -0.3 286,809 + GFF254 0.86 -0.8 286,809 + GFF254 0.86 -0.3 286,809 + GFF254 0.86 -1.6 286,809 + GFF254 0.86 +0.3 286,809 + GFF254 0.86 -2.3 286,809 + GFF254 0.86 -1.1 286,809 + GFF254 0.86 -1.9 286,809 + GFF254 0.86 -0.5 286,810 - GFF254 0.86 +0.1 286,810 - GFF254 0.86 -0.6 286,810 - GFF254 0.86 -2.1 286,810 - GFF254 0.86 -0.3 286,810 - GFF254 0.86 -0.1 286,810 - GFF254 0.86 -0.8 286,810 - GFF254 0.86 +1.3 286,810 - GFF254 0.86 -0.3 286,810 - GFF254 0.86 -2.9 286,836 + GFF254 0.88 -2.7 286,836 + GFF254 0.88 -0.8 286,836 + GFF254 0.88 +0.2 286,836 + GFF254 0.88 -0.9 286,837 - GFF254 0.88 -2.1 286,837 - GFF254 0.88 -2.3 286,837 - GFF254 0.88 -0.7 286,837 - GFF254 0.88 -0.7 286,837 - GFF254 0.88 -1.3 287,423 - GFF255 0.48 -1.9 287,644 + GFF255 0.75 -0.3 287,644 + GFF255 0.75 -1.5 287,644 + GFF255 0.75 -2.6 287,644 + GFF255 0.75 -1.3 287,644 + GFF255 0.75 -0.7 287,693 - GFF255 0.81 -2.0 287,767 + GFF255 0.90 -0.9 287,776 + -1.0 287,776 + -0.7 287,777 - +0.6 287,972 + -0.9 287,972 + +0.8 287,972 + -1.0 287,973 - +0.2 287,973 - +0.4 287,973 - -2.0 287,976 + -0.7 287,976 + +1.3 287,976 + -0.7 287,976 + -0.5 287,976 + -0.5 287,976 + -0.4 287,976 + +0.9 287,976 + +0.3 287,976 + -1.1 287,976 + -0.0 287,976 + -0.4 287,977 - +0.1 287,977 - -0.7 287,977 - +1.1 287,977 - +0.6 287,977 - +1.0 287,977 - +0.4 287,977 - -1.0 287,977 - -1.0 287,977 - -0.3 287,977 - -0.2 287,977 - -1.1 287,977 - +2.1 287,977 - -0.3 287,977 - +0.1 287,977 - +0.9 287,977 - -0.8 287,977 - -2.1 287,977 - -0.4 287,977 - -0.2 287,977 - -1.3 287,977 - -1.1
Or see this region's nucleotide sequence