Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2234 and GFF2235 are separated by 7 nucleotides GFF2235 and GFF2236 overlap by 4 nucleotides GFF2236 and GFF2237 are separated by 70 nucleotides GFF2237 and GFF2238 are separated by 122 nucleotides
GFF2234 - no description, at 295,895 to 296,095
GFF2234
GFF2235 - hypothetical protein, at 296,103 to 296,834
GFF2235
GFF2236 - hypothetical protein, at 296,831 to 297,247
GFF2236
GFF2237 - Intracellular protease, at 297,318 to 297,896
GFF2237
GFF2238 - hypothetical protein, at 298,019 to 298,480
GFF2238
Position (kb) 296
297
298 Strain fitness (log2 ratio) -1
0
1
2 at 296.101 kb on - strand at 296.101 kb on - strand at 296.158 kb on - strand at 296.251 kb on - strand, within GFF2235 at 296.251 kb on - strand, within GFF2235 at 296.251 kb on - strand, within GFF2235 at 296.543 kb on + strand, within GFF2235 at 296.772 kb on + strand at 296.773 kb on - strand at 296.773 kb on - strand at 297.075 kb on + strand, within GFF2236 at 297.210 kb on + strand at 297.210 kb on + strand at 297.295 kb on - strand at 297.442 kb on + strand, within GFF2237 at 297.442 kb on + strand, within GFF2237 at 297.442 kb on + strand, within GFF2237 at 297.443 kb on - strand, within GFF2237 at 297.443 kb on - strand, within GFF2237 at 297.620 kb on - strand, within GFF2237 at 297.659 kb on - strand, within GFF2237 at 297.863 kb on - strand at 297.955 kb on + strand at 297.956 kb on - strand at 298.072 kb on - strand, within GFF2238 at 298.204 kb on - strand, within GFF2238 at 298.243 kb on - strand, within GFF2238 at 298.243 kb on - strand, within GFF2238
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 296,101 - +0.5 296,101 - -0.7 296,158 - -0.1 296,251 - GFF2235 0.20 -1.1 296,251 - GFF2235 0.20 +1.8 296,251 - GFF2235 0.20 +1.3 296,543 + GFF2235 0.60 -0.9 296,772 + +0.1 296,773 - +0.4 296,773 - -0.5 297,075 + GFF2236 0.59 -0.9 297,210 + +0.1 297,210 + -0.1 297,295 - -0.1 297,442 + GFF2237 0.21 +0.2 297,442 + GFF2237 0.21 -1.0 297,442 + GFF2237 0.21 -0.5 297,443 - GFF2237 0.22 -0.7 297,443 - GFF2237 0.22 -0.7 297,620 - GFF2237 0.52 -0.1 297,659 - GFF2237 0.59 +0.4 297,863 - -0.1 297,955 + -0.0 297,956 - +0.4 298,072 - GFF2238 0.11 -0.4 298,204 - GFF2238 0.40 +0.1 298,243 - GFF2238 0.48 +0.3 298,243 - GFF2238 0.48 +0.1
Or see this region's nucleotide sequence