Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2222 and GFF2223 are separated by 139 nucleotides GFF2223 and GFF2224 overlap by 8 nucleotides GFF2224 and GFF2225 overlap by 4 nucleotides
GFF2222 - ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 284,450 to 285,169
GFF2222
GFF2223 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 285,309 to 286,025
GFF2223
GFF2224 - ABC transporter, permease protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 286,018 to 286,884
GFF2224
GFF2225 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 286,881 to 287,924
GFF2225
Position (kb) 286
287 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 285.149 kb on + strand at 285.150 kb on - strand at 285.349 kb on - strand at 285.469 kb on + strand, within GFF2223 at 285.828 kb on + strand, within GFF2223 at 285.829 kb on - strand, within GFF2223 at 285.868 kb on + strand, within GFF2223 at 285.921 kb on + strand, within GFF2223 at 285.922 kb on - strand, within GFF2223 at 285.922 kb on - strand, within GFF2223 at 285.922 kb on - strand, within GFF2223 at 285.922 kb on - strand, within GFF2223 at 285.922 kb on - strand, within GFF2223 at 285.922 kb on - strand, within GFF2223 at 285.922 kb on - strand, within GFF2223 at 285.982 kb on + strand at 285.996 kb on + strand at 285.996 kb on + strand at 285.997 kb on - strand at 286.060 kb on + strand at 286.060 kb on + strand at 286.061 kb on - strand at 286.061 kb on - strand at 286.244 kb on - strand, within GFF2224 at 286.586 kb on - strand, within GFF2224 at 286.889 kb on + strand at 286.889 kb on + strand at 286.889 kb on + strand at 287.046 kb on + strand, within GFF2225 at 287.046 kb on + strand, within GFF2225 at 287.046 kb on + strand, within GFF2225 at 287.047 kb on - strand, within GFF2225 at 287.047 kb on - strand, within GFF2225 at 287.137 kb on - strand, within GFF2225 at 287.137 kb on - strand, within GFF2225 at 287.189 kb on - strand, within GFF2225 at 287.262 kb on + strand, within GFF2225 at 287.263 kb on - strand, within GFF2225 at 287.400 kb on + strand, within GFF2225 at 287.400 kb on + strand, within GFF2225 at 287.401 kb on - strand, within GFF2225 at 287.445 kb on + strand, within GFF2225 at 287.446 kb on - strand, within GFF2225 at 287.446 kb on - strand, within GFF2225 at 287.553 kb on + strand, within GFF2225 at 287.553 kb on + strand, within GFF2225 at 287.553 kb on + strand, within GFF2225 at 287.553 kb on + strand, within GFF2225 at 287.553 kb on + strand, within GFF2225 at 287.554 kb on - strand, within GFF2225 at 287.554 kb on - strand, within GFF2225 at 287.595 kb on + strand, within GFF2225 at 287.595 kb on + strand, within GFF2225 at 287.596 kb on - strand, within GFF2225 at 287.767 kb on - strand, within GFF2225 at 287.767 kb on - strand, within GFF2225
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 285,149 + +0.2 285,150 - +0.2 285,349 - -0.2 285,469 + GFF2223 0.22 +1.2 285,828 + GFF2223 0.72 -3.4 285,829 - GFF2223 0.73 -3.0 285,868 + GFF2223 0.78 +0.1 285,921 + GFF2223 0.85 +0.3 285,922 - GFF2223 0.85 -0.1 285,922 - GFF2223 0.85 +0.8 285,922 - GFF2223 0.85 +0.3 285,922 - GFF2223 0.85 -1.9 285,922 - GFF2223 0.85 +0.7 285,922 - GFF2223 0.85 +0.2 285,922 - GFF2223 0.85 +1.1 285,982 + -0.3 285,996 + +0.4 285,996 + +0.0 285,997 - +0.2 286,060 + +0.8 286,060 + -0.0 286,061 - +0.8 286,061 - -0.9 286,244 - GFF2224 0.26 +1.5 286,586 - GFF2224 0.66 -0.4 286,889 + +0.4 286,889 + -2.0 286,889 + -0.6 287,046 + GFF2225 0.16 -1.4 287,046 + GFF2225 0.16 +0.9 287,046 + GFF2225 0.16 +0.1 287,047 - GFF2225 0.16 +0.1 287,047 - GFF2225 0.16 -0.1 287,137 - GFF2225 0.25 +0.2 287,137 - GFF2225 0.25 -0.2 287,189 - GFF2225 0.30 -1.1 287,262 + GFF2225 0.36 +0.2 287,263 - GFF2225 0.37 -0.6 287,400 + GFF2225 0.50 -0.2 287,400 + GFF2225 0.50 -2.3 287,401 - GFF2225 0.50 +0.6 287,445 + GFF2225 0.54 -1.2 287,446 - GFF2225 0.54 -0.2 287,446 - GFF2225 0.54 +0.8 287,553 + GFF2225 0.64 +0.1 287,553 + GFF2225 0.64 -0.2 287,553 + GFF2225 0.64 +0.1 287,553 + GFF2225 0.64 -0.2 287,553 + GFF2225 0.64 -0.6 287,554 - GFF2225 0.64 -1.5 287,554 - GFF2225 0.64 -0.3 287,595 + GFF2225 0.68 +0.2 287,595 + GFF2225 0.68 +0.1 287,596 - GFF2225 0.68 -1.5 287,767 - GFF2225 0.85 -0.7 287,767 - GFF2225 0.85 +0.2
Or see this region's nucleotide sequence