Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2135 and GFF2136 overlap by 4 nucleotides GFF2136 and GFF2137 overlap by 4 nucleotides GFF2137 and GFF2138 are separated by 36 nucleotides
GFF2135 - D-ribose pyranase (EC 5.4.99.62), at 184,235 to 184,627
GFF2135
GFF2136 - Ribokinase (EC 2.7.1.15), at 184,624 to 185,589
GFF2136
GFF2137 - Ribose operon repressor, at 185,586 to 186,683
GFF2137
GFF2138 - Ribose ABC transporter, permease protein RbsC (TC 3.A.1.2.1), at 186,720 to 187,709
GFF2138
Position (kb) 185
186
187 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 184.741 kb on + strand, within GFF2136 at 184.742 kb on - strand, within GFF2136 at 185.004 kb on - strand, within GFF2136 at 185.203 kb on + strand, within GFF2136 at 185.204 kb on - strand, within GFF2136 at 185.204 kb on - strand, within GFF2136 at 185.448 kb on + strand, within GFF2136 at 185.448 kb on + strand, within GFF2136 at 185.448 kb on + strand, within GFF2136 at 185.828 kb on - strand, within GFF2137 at 186.365 kb on - strand, within GFF2137 at 186.415 kb on + strand, within GFF2137 at 186.466 kb on + strand, within GFF2137 at 186.467 kb on - strand, within GFF2137 at 186.467 kb on - strand, within GFF2137 at 186.467 kb on - strand, within GFF2137 at 186.550 kb on + strand, within GFF2137 at 186.550 kb on + strand, within GFF2137 at 186.550 kb on + strand, within GFF2137 at 186.551 kb on - strand, within GFF2137 at 186.551 kb on - strand, within GFF2137 at 186.701 kb on - strand at 187.270 kb on + strand, within GFF2138 at 187.270 kb on + strand, within GFF2138 at 187.271 kb on - strand, within GFF2138 at 187.582 kb on + strand, within GFF2138 at 187.583 kb on - strand, within GFF2138 at 187.583 kb on - strand, within GFF2138 at 187.642 kb on + strand at 187.642 kb on + strand at 187.642 kb on + strand at 187.642 kb on + strand at 187.642 kb on + strand at 187.642 kb on + strand at 187.643 kb on - strand at 187.643 kb on - strand at 187.643 kb on - strand at 187.643 kb on - strand at 187.643 kb on - strand at 187.643 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 184,741 + GFF2136 0.12 -0.0 184,742 - GFF2136 0.12 +0.2 185,004 - GFF2136 0.39 -2.7 185,203 + GFF2136 0.60 +0.0 185,204 - GFF2136 0.60 +0.8 185,204 - GFF2136 0.60 +0.1 185,448 + GFF2136 0.85 -0.4 185,448 + GFF2136 0.85 -1.3 185,448 + GFF2136 0.85 -0.6 185,828 - GFF2137 0.22 -1.7 186,365 - GFF2137 0.71 -0.2 186,415 + GFF2137 0.76 -1.2 186,466 + GFF2137 0.80 -1.6 186,467 - GFF2137 0.80 -0.6 186,467 - GFF2137 0.80 +1.0 186,467 - GFF2137 0.80 -0.6 186,550 + GFF2137 0.88 -2.6 186,550 + GFF2137 0.88 -0.7 186,550 + GFF2137 0.88 -0.1 186,551 - GFF2137 0.88 -2.5 186,551 - GFF2137 0.88 -1.0 186,701 - +0.8 187,270 + GFF2138 0.56 +0.2 187,270 + GFF2138 0.56 +0.8 187,271 - GFF2138 0.56 -0.7 187,582 + GFF2138 0.87 +0.4 187,583 - GFF2138 0.87 +0.6 187,583 - GFF2138 0.87 -0.1 187,642 + -0.3 187,642 + -3.2 187,642 + -0.7 187,642 + -1.8 187,642 + +0.8 187,642 + -2.1 187,643 - +0.2 187,643 - +0.8 187,643 - +1.4 187,643 - -0.6 187,643 - -0.5 187,643 - -0.3
Or see this region's nucleotide sequence