Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF197 and GFF198 overlap by 4 nucleotides GFF198 and GFF199 are separated by 14 nucleotides GFF199 and GFF200 overlap by 1 nucleotides
GFF197 - BUG/TctC family periplasmic protein, at 225,644 to 226,612
GFF197
GFF198 - Isochorismate pyruvate-lyase (EC 4.2.99.21), at 226,609 to 226,977
GFF198
GFF199 - Ortho-halobenzoate 1,2-dioxygenase beta-ISP protein OhbA, at 226,992 to 227,471
GFF199
GFF200 - Ortho-halobenzoate 1,2-dioxygenase alpha-ISP protein OhbB, at 227,471 to 228,727
GFF200
Position (kb) 226
227
228 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 226.068 kb on + strand, within GFF197 at 226.068 kb on + strand, within GFF197 at 226.068 kb on + strand, within GFF197 at 226.068 kb on + strand, within GFF197 at 226.069 kb on - strand, within GFF197 at 226.309 kb on - strand, within GFF197 at 226.309 kb on - strand, within GFF197 at 226.323 kb on + strand, within GFF197 at 226.324 kb on - strand, within GFF197 at 226.324 kb on - strand, within GFF197 at 226.324 kb on - strand, within GFF197 at 226.359 kb on + strand, within GFF197 at 226.359 kb on + strand, within GFF197 at 226.359 kb on + strand, within GFF197 at 226.359 kb on + strand, within GFF197 at 226.359 kb on + strand, within GFF197 at 226.359 kb on + strand, within GFF197 at 226.360 kb on - strand, within GFF197 at 226.360 kb on - strand, within GFF197 at 226.360 kb on - strand, within GFF197 at 226.713 kb on - strand, within GFF198 at 226.916 kb on + strand, within GFF198 at 226.916 kb on + strand, within GFF198 at 226.916 kb on + strand, within GFF198 at 226.916 kb on + strand, within GFF198 at 226.916 kb on + strand, within GFF198 at 226.917 kb on - strand, within GFF198 at 226.917 kb on - strand, within GFF198 at 227.095 kb on + strand, within GFF199 at 227.095 kb on + strand, within GFF199 at 227.096 kb on - strand, within GFF199 at 227.152 kb on + strand, within GFF199 at 227.152 kb on + strand, within GFF199 at 227.153 kb on - strand, within GFF199 at 227.153 kb on - strand, within GFF199 at 227.228 kb on - strand, within GFF199 at 227.228 kb on - strand, within GFF199 at 227.228 kb on - strand, within GFF199 at 227.228 kb on - strand, within GFF199 at 227.230 kb on + strand, within GFF199 at 227.231 kb on - strand, within GFF199 at 227.231 kb on - strand, within GFF199 at 227.263 kb on + strand, within GFF199 at 227.353 kb on + strand, within GFF199 at 227.353 kb on + strand, within GFF199 at 227.354 kb on - strand, within GFF199 at 227.419 kb on + strand, within GFF199 at 227.429 kb on - strand at 227.429 kb on - strand at 227.449 kb on + strand at 227.449 kb on + strand at 227.450 kb on - strand at 227.450 kb on - strand at 227.502 kb on + strand at 227.502 kb on + strand at 227.502 kb on + strand at 227.502 kb on + strand at 227.502 kb on + strand at 227.502 kb on + strand at 227.502 kb on + strand at 227.502 kb on + strand at 227.502 kb on + strand at 227.502 kb on + strand at 227.502 kb on + strand at 227.502 kb on + strand at 227.502 kb on + strand at 227.503 kb on - strand at 227.503 kb on - strand at 227.503 kb on - strand at 227.503 kb on - strand at 227.503 kb on - strand at 227.586 kb on + strand at 228.133 kb on - strand, within GFF200 at 228.178 kb on - strand, within GFF200 at 228.387 kb on + strand, within GFF200 at 228.387 kb on + strand, within GFF200 at 228.388 kb on - strand, within GFF200 at 228.388 kb on - strand, within GFF200 at 228.388 kb on - strand, within GFF200
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 226,068 + GFF197 0.44 +0.3 226,068 + GFF197 0.44 +0.5 226,068 + GFF197 0.44 +0.7 226,068 + GFF197 0.44 -0.3 226,069 - GFF197 0.44 -0.3 226,309 - GFF197 0.69 +0.1 226,309 - GFF197 0.69 +0.8 226,323 + GFF197 0.70 -0.3 226,324 - GFF197 0.70 +0.1 226,324 - GFF197 0.70 -1.0 226,324 - GFF197 0.70 -0.3 226,359 + GFF197 0.74 -1.0 226,359 + GFF197 0.74 -0.7 226,359 + GFF197 0.74 +1.3 226,359 + GFF197 0.74 -2.3 226,359 + GFF197 0.74 -1.3 226,359 + GFF197 0.74 +1.9 226,360 - GFF197 0.74 +0.6 226,360 - GFF197 0.74 -0.7 226,360 - GFF197 0.74 -2.1 226,713 - GFF198 0.28 +0.3 226,916 + GFF198 0.83 -0.7 226,916 + GFF198 0.83 +1.3 226,916 + GFF198 0.83 +1.1 226,916 + GFF198 0.83 +0.6 226,916 + GFF198 0.83 +0.0 226,917 - GFF198 0.83 +0.7 226,917 - GFF198 0.83 -0.7 227,095 + GFF199 0.21 -0.6 227,095 + GFF199 0.21 -0.9 227,096 - GFF199 0.22 -0.3 227,152 + GFF199 0.33 +0.6 227,152 + GFF199 0.33 -0.1 227,153 - GFF199 0.34 -0.6 227,153 - GFF199 0.34 +0.7 227,228 - GFF199 0.49 -1.2 227,228 - GFF199 0.49 -0.3 227,228 - GFF199 0.49 -1.7 227,228 - GFF199 0.49 -0.6 227,230 + GFF199 0.50 -0.3 227,231 - GFF199 0.50 +1.0 227,231 - GFF199 0.50 +2.2 227,263 + GFF199 0.56 +0.2 227,353 + GFF199 0.75 -1.0 227,353 + GFF199 0.75 +0.3 227,354 - GFF199 0.75 -1.0 227,419 + GFF199 0.89 -0.6 227,429 - -2.0 227,429 - +0.3 227,449 + -2.0 227,449 + +0.5 227,450 - -0.1 227,450 - -1.1 227,502 + +3.9 227,502 + +0.7 227,502 + +0.9 227,502 + -0.9 227,502 + +0.1 227,502 + +0.7 227,502 + +0.7 227,502 + -1.1 227,502 + -1.0 227,502 + +0.6 227,502 + -0.3 227,502 + -0.1 227,502 + -0.3 227,503 - -0.3 227,503 - +0.2 227,503 - +0.2 227,503 - +1.1 227,503 - -0.6 227,586 + -0.6 228,133 - GFF200 0.53 -0.8 228,178 - GFF200 0.56 -0.3 228,387 + GFF200 0.73 +1.2 228,387 + GFF200 0.73 +0.7 228,388 - GFF200 0.73 +0.5 228,388 - GFF200 0.73 +0.3 228,388 - GFF200 0.73 -0.2
Or see this region's nucleotide sequence