Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1473 and GFF1474 are separated by 114 nucleotides GFF1474 and GFF1475 are separated by 34 nucleotides GFF1475 and GFF1476 are separated by 89 nucleotides
GFF1473 - Amidase family protein BBta_1912, at 64,651 to 66,060
GFF1473
GFF1474 - Transcriptional regulator, LysR family, at 66,175 to 67,074
GFF1474
GFF1475 - no description, at 67,109 to 67,747
GFF1475
GFF1476 - hypothetical protein, at 67,837 to 68,583
GFF1476
Position (kb)
66
67
68 Strain fitness (log2 ratio)
-2
-1
0
1 at 65.441 kb on + strand, within GFF1473 at 65.442 kb on - strand, within GFF1473 at 65.442 kb on - strand, within GFF1473 at 65.447 kb on + strand, within GFF1473 at 65.447 kb on + strand, within GFF1473 at 65.448 kb on - strand, within GFF1473 at 65.448 kb on - strand, within GFF1473 at 65.805 kb on - strand, within GFF1473 at 65.815 kb on + strand, within GFF1473 at 65.815 kb on + strand, within GFF1473 at 65.816 kb on - strand, within GFF1473 at 65.938 kb on + strand at 66.049 kb on + strand at 66.163 kb on + strand at 66.163 kb on + strand at 66.175 kb on - strand at 66.206 kb on - strand at 66.490 kb on + strand, within GFF1474 at 66.490 kb on + strand, within GFF1474 at 66.490 kb on + strand, within GFF1474 at 66.553 kb on + strand, within GFF1474 at 67.073 kb on - strand at 67.073 kb on - strand at 67.073 kb on - strand at 67.175 kb on - strand, within GFF1475 at 67.318 kb on - strand, within GFF1475 at 67.807 kb on + strand at 67.807 kb on + strand at 67.808 kb on - strand at 67.808 kb on - strand at 67.849 kb on + strand at 67.849 kb on + strand at 67.850 kb on - strand at 67.953 kb on - strand, within GFF1476 at 67.953 kb on - strand, within GFF1476 at 68.068 kb on + strand, within GFF1476 at 68.069 kb on - strand, within GFF1476 at 68.069 kb on - strand, within GFF1476 at 68.069 kb on - strand, within GFF1476 at 68.069 kb on - strand, within GFF1476 at 68.072 kb on + strand, within GFF1476 at 68.072 kb on + strand, within GFF1476 at 68.073 kb on - strand, within GFF1476
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 65,441 + GFF1473 0.56 -1.0 65,442 - GFF1473 0.56 +1.3 65,442 - GFF1473 0.56 +0.7 65,447 + GFF1473 0.56 +0.4 65,447 + GFF1473 0.56 +0.5 65,448 - GFF1473 0.57 -0.5 65,448 - GFF1473 0.57 +0.1 65,805 - GFF1473 0.82 -1.8 65,815 + GFF1473 0.83 -0.1 65,815 + GFF1473 0.83 +0.3 65,816 - GFF1473 0.83 -0.2 65,938 + -2.3 66,049 + -0.3 66,163 + +0.2 66,163 + +1.7 66,175 - -0.3 66,206 - -1.2 66,490 + GFF1474 0.35 -1.4 66,490 + GFF1474 0.35 -0.3 66,490 + GFF1474 0.35 -0.3 66,553 + GFF1474 0.42 -0.9 67,073 - +0.2 67,073 - -0.3 67,073 - +0.2 67,175 - GFF1475 0.10 -1.3 67,318 - GFF1475 0.33 -1.0 67,807 + -0.3 67,807 + +0.6 67,808 - +0.4 67,808 - -0.6 67,849 + +0.7 67,849 + -0.3 67,850 - +1.5 67,953 - GFF1476 0.16 +0.6 67,953 - GFF1476 0.16 -0.6 68,068 + GFF1476 0.31 +0.0 68,069 - GFF1476 0.31 -0.8 68,069 - GFF1476 0.31 +0.5 68,069 - GFF1476 0.31 -0.3 68,069 - GFF1476 0.31 +0.7 68,072 + GFF1476 0.31 -0.0 68,072 + GFF1476 0.31 -1.5 68,073 - GFF1476 0.32 +0.2
Or see this region's nucleotide sequence