Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1421 and GFF1422 are separated by 29 nucleotides GFF1422 and GFF1423 overlap by 8 nucleotides GFF1423 and GFF1424 overlap by 4 nucleotides
GFF1421 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 12,087 to 13,241
GFF1421
GFF1422 - L-lactate dehydrogenase, at 13,271 to 14,431
GFF1422
GFF1423 - Phenylacetate ABC transporter, permease protein 1, at 14,424 to 15,296
GFF1423
GFF1424 - Phenylacetate ABC transporter, permease protein 2, at 15,293 to 16,225
GFF1424
Position (kb) 13
14
15 Strain fitness (log2 ratio) -1
0
1 at 12.804 kb on + strand, within GFF1421 at 12.804 kb on + strand, within GFF1421 at 12.804 kb on + strand, within GFF1421 at 12.896 kb on + strand, within GFF1421 at 12.976 kb on - strand, within GFF1421 at 12.987 kb on + strand, within GFF1421 at 13.149 kb on + strand at 13.337 kb on + strand at 13.830 kb on + strand, within GFF1422 at 14.399 kb on + strand at 14.508 kb on + strand at 14.508 kb on + strand at 14.508 kb on + strand at 14.509 kb on - strand at 15.063 kb on + strand, within GFF1423 at 15.189 kb on + strand, within GFF1423 at 15.190 kb on - strand, within GFF1423 at 15.204 kb on + strand, within GFF1423 at 15.292 kb on + strand at 15.292 kb on + strand at 15.293 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 12,804 + GFF1421 0.62 -0.5 12,804 + GFF1421 0.62 -0.3 12,804 + GFF1421 0.62 +0.5 12,896 + GFF1421 0.70 -0.6 12,976 - GFF1421 0.77 -0.0 12,987 + GFF1421 0.78 -1.2 13,149 + -0.2 13,337 + -0.1 13,830 + GFF1422 0.48 +0.1 14,399 + -0.7 14,508 + -0.1 14,508 + +0.7 14,508 + +0.0 14,509 - +0.2 15,063 + GFF1423 0.73 -1.0 15,189 + GFF1423 0.88 +0.1 15,190 - GFF1423 0.88 +0.1 15,204 + GFF1423 0.89 -1.6 15,292 + -0.2 15,292 + -0.8 15,293 - -0.4
Or see this region's nucleotide sequence