Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1323 and GFF1324 are separated by 155 nucleotides GFF1324 and GFF1325 are separated by 5 nucleotides GFF1325 and GFF1326 are separated by 63 nucleotides
GFF1323 - Methionine aminopeptidase (EC 3.4.11.18), at 661,241 to 662,065
GFF1323
GFF1324 - Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3), at 662,221 to 666,189
GFF1324
GFF1325 - no description, at 666,195 to 666,506
GFF1325
GFF1326 - Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8), at 666,570 to 667,355
GFF1326
Position (kb) 662
663
664
665
666
667 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 661.389 kb on + strand, within GFF1323 at 661.389 kb on + strand, within GFF1323 at 661.389 kb on + strand, within GFF1323 at 661.389 kb on + strand, within GFF1323 at 661.390 kb on - strand, within GFF1323 at 661.390 kb on - strand, within GFF1323 at 661.390 kb on - strand, within GFF1323 at 661.500 kb on + strand, within GFF1323 at 661.501 kb on - strand, within GFF1323 at 661.501 kb on - strand, within GFF1323 at 661.501 kb on - strand, within GFF1323 at 661.719 kb on + strand, within GFF1323 at 661.719 kb on + strand, within GFF1323 at 661.719 kb on + strand, within GFF1323 at 661.719 kb on + strand, within GFF1323 at 661.719 kb on + strand, within GFF1323 at 661.719 kb on + strand, within GFF1323 at 661.719 kb on + strand, within GFF1323 at 661.719 kb on + strand, within GFF1323 at 661.719 kb on + strand, within GFF1323 at 661.719 kb on + strand, within GFF1323 at 661.719 kb on + strand, within GFF1323 at 661.719 kb on + strand, within GFF1323 at 661.719 kb on + strand, within GFF1323 at 661.719 kb on + strand, within GFF1323 at 661.719 kb on + strand, within GFF1323 at 661.720 kb on - strand, within GFF1323 at 661.720 kb on - strand, within GFF1323 at 661.720 kb on - strand, within GFF1323 at 661.720 kb on - strand, within GFF1323 at 661.720 kb on - strand, within GFF1323 at 661.720 kb on - strand, within GFF1323 at 661.720 kb on - strand, within GFF1323 at 661.720 kb on - strand, within GFF1323 at 661.720 kb on - strand, within GFF1323 at 661.720 kb on - strand, within GFF1323 at 661.720 kb on - strand, within GFF1323 at 661.720 kb on - strand, within GFF1323 at 661.720 kb on - strand, within GFF1323 at 661.720 kb on - strand, within GFF1323 at 661.884 kb on + strand, within GFF1323 at 661.884 kb on + strand, within GFF1323 at 661.884 kb on + strand, within GFF1323 at 661.884 kb on + strand, within GFF1323 at 661.884 kb on + strand, within GFF1323 at 661.885 kb on - strand, within GFF1323 at 661.885 kb on - strand, within GFF1323 at 661.885 kb on - strand, within GFF1323 at 661.885 kb on - strand, within GFF1323 at 661.885 kb on - strand, within GFF1323 at 661.885 kb on - strand, within GFF1323 at 661.885 kb on - strand, within GFF1323 at 661.885 kb on - strand, within GFF1323 at 661.923 kb on + strand, within GFF1323 at 661.923 kb on + strand, within GFF1323 at 661.923 kb on + strand, within GFF1323 at 661.923 kb on + strand, within GFF1323 at 661.923 kb on + strand, within GFF1323 at 661.923 kb on + strand, within GFF1323 at 661.923 kb on + strand, within GFF1323 at 661.923 kb on + strand, within GFF1323 at 661.924 kb on - strand, within GFF1323 at 661.924 kb on - strand, within GFF1323 at 661.924 kb on - strand, within GFF1323 at 661.924 kb on - strand, within GFF1323 at 661.924 kb on - strand, within GFF1323 at 661.924 kb on - strand, within GFF1323 at 661.924 kb on - strand, within GFF1323 at 661.924 kb on - strand, within GFF1323 at 661.924 kb on - strand, within GFF1323 at 661.924 kb on - strand, within GFF1323 at 661.924 kb on - strand, within GFF1323 at 661.924 kb on - strand, within GFF1323 at 661.924 kb on - strand, within GFF1323 at 661.924 kb on - strand, within GFF1323 at 661.924 kb on - strand, within GFF1323 at 661.924 kb on - strand, within GFF1323 at 661.924 kb on - strand, within GFF1323 at 661.986 kb on + strand at 661.986 kb on + strand at 661.986 kb on + strand at 661.986 kb on + strand at 661.986 kb on + strand at 661.987 kb on - strand at 661.987 kb on - strand at 661.987 kb on - strand at 661.987 kb on - strand at 661.987 kb on - strand at 661.987 kb on - strand at 662.052 kb on + strand at 662.052 kb on + strand at 662.052 kb on + strand at 662.053 kb on - strand at 662.053 kb on - strand at 662.053 kb on - strand at 662.053 kb on - strand at 662.053 kb on - strand at 662.053 kb on - strand at 662.056 kb on + strand at 662.056 kb on + strand at 662.056 kb on + strand at 662.056 kb on + strand at 662.056 kb on + strand at 662.056 kb on + strand at 662.057 kb on - strand at 662.057 kb on - strand at 662.057 kb on - strand at 662.057 kb on - strand at 662.057 kb on - strand at 662.057 kb on - strand at 662.057 kb on - strand at 662.140 kb on + strand at 662.140 kb on + strand at 662.141 kb on - strand at 662.141 kb on - strand at 662.216 kb on + strand at 662.216 kb on + strand at 662.216 kb on + strand at 662.216 kb on + strand at 662.216 kb on + strand at 662.392 kb on + strand at 662.393 kb on - strand at 662.427 kb on + strand at 662.428 kb on - strand at 662.542 kb on + strand at 662.542 kb on + strand at 662.543 kb on - strand at 662.797 kb on + strand, within GFF1324 at 662.798 kb on - strand, within GFF1324 at 662.998 kb on + strand, within GFF1324 at 662.998 kb on + strand, within GFF1324 at 662.998 kb on + strand, within GFF1324 at 662.998 kb on + strand, within GFF1324 at 662.999 kb on - strand, within GFF1324 at 663.376 kb on + strand, within GFF1324 at 663.377 kb on - strand, within GFF1324 at 663.388 kb on + strand, within GFF1324 at 663.388 kb on + strand, within GFF1324 at 663.389 kb on - strand, within GFF1324 at 663.434 kb on - strand, within GFF1324 at 663.865 kb on + strand, within GFF1324 at 663.865 kb on + strand, within GFF1324 at 663.866 kb on - strand, within GFF1324 at 664.273 kb on + strand, within GFF1324 at 664.273 kb on + strand, within GFF1324 at 664.273 kb on + strand, within GFF1324 at 665.486 kb on - strand, within GFF1324 at 665.486 kb on - strand, within GFF1324 at 665.599 kb on + strand, within GFF1324 at 665.600 kb on - strand, within GFF1324 at 665.999 kb on - strand at 665.999 kb on - strand at 666.346 kb on + strand, within GFF1325 at 666.346 kb on + strand, within GFF1325 at 666.347 kb on - strand, within GFF1325 at 666.347 kb on - strand, within GFF1325 at 666.347 kb on - strand, within GFF1325 at 666.390 kb on + strand, within GFF1325 at 666.390 kb on + strand, within GFF1325 at 666.390 kb on + strand, within GFF1325 at 666.390 kb on + strand, within GFF1325 at 666.390 kb on + strand, within GFF1325 at 666.390 kb on + strand, within GFF1325 at 666.390 kb on + strand, within GFF1325 at 666.390 kb on + strand, within GFF1325 at 666.390 kb on + strand, within GFF1325 at 666.390 kb on + strand, within GFF1325 at 666.390 kb on + strand, within GFF1325 at 666.390 kb on + strand, within GFF1325 at 666.391 kb on - strand, within GFF1325 at 666.391 kb on - strand, within GFF1325 at 666.391 kb on - strand, within GFF1325 at 666.391 kb on - strand, within GFF1325 at 666.391 kb on - strand, within GFF1325 at 666.572 kb on - strand at 666.607 kb on + strand at 666.607 kb on + strand at 666.607 kb on + strand at 666.607 kb on + strand at 666.607 kb on + strand at 666.607 kb on + strand at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.955 kb on + strand, within GFF1326 at 666.956 kb on - strand, within GFF1326 at 666.956 kb on - strand, within GFF1326 at 666.956 kb on - strand, within GFF1326 at 666.956 kb on - strand, within GFF1326 at 666.956 kb on - strand, within GFF1326 at 666.956 kb on - strand, within GFF1326 at 666.956 kb on - strand, within GFF1326 at 666.956 kb on - strand, within GFF1326 at 666.991 kb on + strand, within GFF1326 at 666.992 kb on - strand, within GFF1326 at 667.036 kb on + strand, within GFF1326 at 667.036 kb on + strand, within GFF1326 at 667.036 kb on + strand, within GFF1326 at 667.036 kb on + strand, within GFF1326 at 667.036 kb on + strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.037 kb on - strand, within GFF1326 at 667.096 kb on + strand, within GFF1326 at 667.096 kb on + strand, within GFF1326 at 667.096 kb on + strand, within GFF1326 at 667.097 kb on - strand, within GFF1326 at 667.097 kb on - strand, within GFF1326 at 667.097 kb on - strand, within GFF1326 at 667.097 kb on - strand, within GFF1326
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 661,389 + GFF1323 0.18 +0.3 661,389 + GFF1323 0.18 -0.5 661,389 + GFF1323 0.18 +0.4 661,389 + GFF1323 0.18 +0.6 661,390 - GFF1323 0.18 -0.5 661,390 - GFF1323 0.18 -1.4 661,390 - GFF1323 0.18 -1.0 661,500 + GFF1323 0.31 +1.1 661,501 - GFF1323 0.32 +0.3 661,501 - GFF1323 0.32 -0.9 661,501 - GFF1323 0.32 +0.1 661,719 + GFF1323 0.58 -0.4 661,719 + GFF1323 0.58 -0.4 661,719 + GFF1323 0.58 +1.2 661,719 + GFF1323 0.58 -0.7 661,719 + GFF1323 0.58 +1.8 661,719 + GFF1323 0.58 -0.9 661,719 + GFF1323 0.58 -1.5 661,719 + GFF1323 0.58 -0.2 661,719 + GFF1323 0.58 +0.3 661,719 + GFF1323 0.58 +0.0 661,719 + GFF1323 0.58 +0.6 661,719 + GFF1323 0.58 -0.1 661,719 + GFF1323 0.58 +0.1 661,719 + GFF1323 0.58 -0.6 661,719 + GFF1323 0.58 -0.1 661,720 - GFF1323 0.58 -0.4 661,720 - GFF1323 0.58 -0.8 661,720 - GFF1323 0.58 +0.1 661,720 - GFF1323 0.58 -1.4 661,720 - GFF1323 0.58 +0.3 661,720 - GFF1323 0.58 -1.0 661,720 - GFF1323 0.58 +0.5 661,720 - GFF1323 0.58 -0.7 661,720 - GFF1323 0.58 -0.1 661,720 - GFF1323 0.58 -2.0 661,720 - GFF1323 0.58 -0.6 661,720 - GFF1323 0.58 -0.4 661,720 - GFF1323 0.58 +1.4 661,720 - GFF1323 0.58 -0.8 661,884 + GFF1323 0.78 -0.2 661,884 + GFF1323 0.78 -0.3 661,884 + GFF1323 0.78 -1.0 661,884 + GFF1323 0.78 +0.6 661,884 + GFF1323 0.78 -0.6 661,885 - GFF1323 0.78 -0.6 661,885 - GFF1323 0.78 -0.7 661,885 - GFF1323 0.78 +0.8 661,885 - GFF1323 0.78 -0.1 661,885 - GFF1323 0.78 +2.4 661,885 - GFF1323 0.78 -0.2 661,885 - GFF1323 0.78 +0.8 661,885 - GFF1323 0.78 +0.5 661,923 + GFF1323 0.83 -0.2 661,923 + GFF1323 0.83 +1.4 661,923 + GFF1323 0.83 -1.4 661,923 + GFF1323 0.83 +0.3 661,923 + GFF1323 0.83 -0.6 661,923 + GFF1323 0.83 +0.2 661,923 + GFF1323 0.83 +0.3 661,923 + GFF1323 0.83 -0.6 661,924 - GFF1323 0.83 +0.8 661,924 - GFF1323 0.83 +1.2 661,924 - GFF1323 0.83 +0.6 661,924 - GFF1323 0.83 -0.1 661,924 - GFF1323 0.83 +0.8 661,924 - GFF1323 0.83 +2.8 661,924 - GFF1323 0.83 +0.7 661,924 - GFF1323 0.83 -0.7 661,924 - GFF1323 0.83 -0.4 661,924 - GFF1323 0.83 -0.2 661,924 - GFF1323 0.83 +0.6 661,924 - GFF1323 0.83 -0.2 661,924 - GFF1323 0.83 +0.4 661,924 - GFF1323 0.83 -2.7 661,924 - GFF1323 0.83 -0.7 661,924 - GFF1323 0.83 +1.8 661,924 - GFF1323 0.83 -0.6 661,986 + +0.2 661,986 + -0.5 661,986 + +0.3 661,986 + -0.0 661,986 + -0.4 661,987 - -1.4 661,987 - +0.5 661,987 - -0.2 661,987 - -0.9 661,987 - -0.1 661,987 - -0.1 662,052 + -1.0 662,052 + +0.2 662,052 + -0.2 662,053 - +1.2 662,053 - +0.1 662,053 - -0.2 662,053 - +0.1 662,053 - +0.6 662,053 - +0.1 662,056 + +0.2 662,056 + -0.9 662,056 + -1.4 662,056 + +1.3 662,056 + -0.0 662,056 + -0.3 662,057 - -2.2 662,057 - +0.5 662,057 - -0.2 662,057 - -0.6 662,057 - +0.9 662,057 - -0.4 662,057 - +0.4 662,140 + +0.8 662,140 + +0.1 662,141 - +0.2 662,141 - -1.3 662,216 + +0.4 662,216 + -0.2 662,216 + +0.6 662,216 + -1.0 662,216 + +0.6 662,392 + -1.9 662,393 - -1.4 662,427 + -0.2 662,428 - -0.9 662,542 + -1.4 662,542 + -0.7 662,543 - +0.4 662,797 + GFF1324 0.15 -1.6 662,798 - GFF1324 0.15 -0.2 662,998 + GFF1324 0.20 -1.4 662,998 + GFF1324 0.20 +0.8 662,998 + GFF1324 0.20 -1.2 662,998 + GFF1324 0.20 -0.6 662,999 - GFF1324 0.20 -0.9 663,376 + GFF1324 0.29 +0.4 663,377 - GFF1324 0.29 -2.3 663,388 + GFF1324 0.29 +0.4 663,388 + GFF1324 0.29 -2.0 663,389 - GFF1324 0.29 -0.2 663,434 - GFF1324 0.31 -0.9 663,865 + GFF1324 0.41 -0.2 663,865 + GFF1324 0.41 +0.4 663,866 - GFF1324 0.41 -0.6 664,273 + GFF1324 0.52 -0.9 664,273 + GFF1324 0.52 +1.4 664,273 + GFF1324 0.52 -1.6 665,486 - GFF1324 0.82 +0.4 665,486 - GFF1324 0.82 -1.7 665,599 + GFF1324 0.85 -0.2 665,600 - GFF1324 0.85 -0.2 665,999 - +0.4 665,999 - -1.6 666,346 + GFF1325 0.48 +0.3 666,346 + GFF1325 0.48 -1.0 666,347 - GFF1325 0.49 -0.5 666,347 - GFF1325 0.49 -0.2 666,347 - GFF1325 0.49 -0.5 666,390 + GFF1325 0.62 +0.6 666,390 + GFF1325 0.62 -1.4 666,390 + GFF1325 0.62 -0.2 666,390 + GFF1325 0.62 -0.2 666,390 + GFF1325 0.62 -0.0 666,390 + GFF1325 0.62 +0.1 666,390 + GFF1325 0.62 +0.2 666,390 + GFF1325 0.62 +0.2 666,390 + GFF1325 0.62 +0.7 666,390 + GFF1325 0.62 -0.8 666,390 + GFF1325 0.62 -2.2 666,390 + GFF1325 0.62 +1.4 666,391 - GFF1325 0.63 +0.5 666,391 - GFF1325 0.63 +2.1 666,391 - GFF1325 0.63 -0.3 666,391 - GFF1325 0.63 +0.4 666,391 - GFF1325 0.63 -0.7 666,572 - -0.9 666,607 + -0.6 666,607 + -0.1 666,607 + -0.3 666,607 + -0.2 666,607 + +0.7 666,607 + +0.0 666,955 + GFF1326 0.49 +0.0 666,955 + GFF1326 0.49 +2.1 666,955 + GFF1326 0.49 -0.2 666,955 + GFF1326 0.49 +0.7 666,955 + GFF1326 0.49 +0.6 666,955 + GFF1326 0.49 +0.8 666,955 + GFF1326 0.49 -0.4 666,955 + GFF1326 0.49 -0.2 666,955 + GFF1326 0.49 +1.4 666,955 + GFF1326 0.49 -1.2 666,956 - GFF1326 0.49 -0.8 666,956 - GFF1326 0.49 -1.4 666,956 - GFF1326 0.49 -1.0 666,956 - GFF1326 0.49 -0.2 666,956 - GFF1326 0.49 -2.6 666,956 - GFF1326 0.49 -1.9 666,956 - GFF1326 0.49 -0.2 666,956 - GFF1326 0.49 -1.1 666,991 + GFF1326 0.54 -1.9 666,992 - GFF1326 0.54 -0.2 667,036 + GFF1326 0.59 -0.6 667,036 + GFF1326 0.59 -0.4 667,036 + GFF1326 0.59 +0.3 667,036 + GFF1326 0.59 -0.5 667,036 + GFF1326 0.59 -1.6 667,037 - GFF1326 0.59 +1.2 667,037 - GFF1326 0.59 -0.9 667,037 - GFF1326 0.59 -0.3 667,037 - GFF1326 0.59 +0.4 667,037 - GFF1326 0.59 -0.3 667,037 - GFF1326 0.59 -0.4 667,037 - GFF1326 0.59 +0.4 667,037 - GFF1326 0.59 -0.6 667,037 - GFF1326 0.59 -0.4 667,037 - GFF1326 0.59 -0.5 667,096 + GFF1326 0.67 -0.4 667,096 + GFF1326 0.67 -0.2 667,096 + GFF1326 0.67 -0.3 667,097 - GFF1326 0.67 -0.8 667,097 - GFF1326 0.67 -1.3 667,097 - GFF1326 0.67 -0.9 667,097 - GFF1326 0.67 -1.5
Or see this region's nucleotide sequence