Experiment: M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1307 and GFF1308 are separated by 6 nucleotides GFF1308 and GFF1309 are separated by 4 nucleotides GFF1309 and GFF1310 overlap by 4 nucleotides GFF1310 and GFF1311 are separated by 160 nucleotides
GFF1307 - Homoserine dehydrogenase (EC 1.1.1.3), at 637,112 to 638,476
GFF1307
GFF1308 - hypothetical protein, at 638,483 to 638,881
GFF1308
GFF1309 - Phosphoglucomutase (EC 5.4.2.2), at 638,886 to 640,541
GFF1309
GFF1310 - hypothetical protein, at 640,538 to 641,215
GFF1310
GFF1311 - no description, at 641,376 to 641,711
GFF1311
Position (kb)
638
639
640
641 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 638.054 kb on + strand, within GFF1307 at 638.054 kb on + strand, within GFF1307 at 638.054 kb on + strand, within GFF1307 at 638.054 kb on + strand, within GFF1307 at 638.054 kb on + strand, within GFF1307 at 638.054 kb on + strand, within GFF1307 at 638.054 kb on + strand, within GFF1307 at 638.054 kb on + strand, within GFF1307 at 638.054 kb on + strand, within GFF1307 at 638.055 kb on - strand, within GFF1307 at 638.055 kb on - strand, within GFF1307 at 638.055 kb on - strand, within GFF1307 at 638.055 kb on - strand, within GFF1307 at 638.055 kb on - strand, within GFF1307 at 638.055 kb on - strand, within GFF1307 at 638.055 kb on - strand, within GFF1307 at 638.055 kb on - strand, within GFF1307 at 638.055 kb on - strand, within GFF1307 at 638.057 kb on + strand, within GFF1307 at 638.057 kb on + strand, within GFF1307 at 638.057 kb on + strand, within GFF1307 at 638.058 kb on - strand, within GFF1307 at 638.058 kb on - strand, within GFF1307 at 638.231 kb on + strand, within GFF1307 at 638.231 kb on + strand, within GFF1307 at 638.231 kb on + strand, within GFF1307 at 638.232 kb on - strand, within GFF1307 at 638.232 kb on - strand, within GFF1307 at 638.514 kb on + strand at 638.514 kb on + strand at 638.515 kb on - strand at 638.974 kb on + strand at 638.974 kb on + strand at 638.975 kb on - strand at 638.983 kb on + strand at 638.983 kb on + strand at 638.983 kb on + strand at 638.983 kb on + strand at 638.983 kb on + strand at 638.983 kb on + strand at 638.983 kb on + strand at 638.984 kb on - strand at 638.984 kb on - strand at 638.984 kb on - strand at 638.984 kb on - strand at 638.984 kb on - strand at 638.984 kb on - strand at 638.984 kb on - strand at 638.984 kb on - strand at 638.984 kb on - strand at 638.984 kb on - strand at 638.984 kb on - strand at 639.230 kb on - strand, within GFF1309 at 639.427 kb on + strand, within GFF1309 at 639.427 kb on + strand, within GFF1309 at 639.428 kb on - strand, within GFF1309 at 639.428 kb on - strand, within GFF1309 at 639.428 kb on - strand, within GFF1309 at 639.428 kb on - strand, within GFF1309 at 639.428 kb on - strand, within GFF1309 at 639.428 kb on - strand, within GFF1309 at 639.428 kb on - strand, within GFF1309 at 639.428 kb on - strand, within GFF1309 at 639.428 kb on - strand, within GFF1309 at 639.428 kb on - strand, within GFF1309 at 639.529 kb on + strand, within GFF1309 at 639.529 kb on + strand, within GFF1309 at 639.530 kb on - strand, within GFF1309 at 639.535 kb on + strand, within GFF1309 at 639.535 kb on + strand, within GFF1309 at 639.535 kb on + strand, within GFF1309 at 639.535 kb on + strand, within GFF1309 at 639.536 kb on - strand, within GFF1309 at 639.536 kb on - strand, within GFF1309 at 639.556 kb on + strand, within GFF1309 at 639.557 kb on - strand, within GFF1309 at 639.781 kb on + strand, within GFF1309 at 639.782 kb on - strand, within GFF1309 at 639.782 kb on - strand, within GFF1309 at 639.782 kb on - strand, within GFF1309 at 639.806 kb on - strand, within GFF1309 at 639.806 kb on - strand, within GFF1309 at 639.901 kb on + strand, within GFF1309 at 639.901 kb on + strand, within GFF1309 at 639.901 kb on + strand, within GFF1309 at 639.901 kb on + strand, within GFF1309 at 639.902 kb on - strand, within GFF1309 at 639.902 kb on - strand, within GFF1309 at 639.902 kb on - strand, within GFF1309 at 639.902 kb on - strand, within GFF1309 at 639.902 kb on - strand, within GFF1309 at 639.902 kb on - strand, within GFF1309 at 639.902 kb on - strand, within GFF1309 at 639.902 kb on - strand, within GFF1309 at 639.902 kb on - strand, within GFF1309 at 639.913 kb on + strand, within GFF1309 at 639.913 kb on + strand, within GFF1309 at 639.914 kb on - strand, within GFF1309 at 640.063 kb on + strand, within GFF1309 at 640.063 kb on + strand, within GFF1309 at 640.063 kb on + strand, within GFF1309 at 640.063 kb on + strand, within GFF1309 at 640.063 kb on + strand, within GFF1309 at 640.064 kb on - strand, within GFF1309 at 640.064 kb on - strand, within GFF1309 at 640.064 kb on - strand, within GFF1309 at 640.064 kb on - strand, within GFF1309 at 640.064 kb on - strand, within GFF1309 at 640.064 kb on - strand, within GFF1309 at 640.148 kb on - strand, within GFF1309 at 640.186 kb on + strand, within GFF1309 at 640.186 kb on + strand, within GFF1309 at 640.187 kb on - strand, within GFF1309 at 640.187 kb on - strand, within GFF1309 at 640.187 kb on - strand, within GFF1309 at 640.456 kb on + strand at 640.456 kb on + strand at 640.456 kb on + strand at 640.456 kb on + strand at 640.456 kb on + strand at 640.456 kb on + strand at 640.457 kb on - strand at 640.457 kb on - strand at 640.457 kb on - strand at 640.457 kb on - strand at 640.457 kb on - strand at 640.459 kb on + strand at 640.459 kb on + strand at 640.460 kb on - strand at 640.605 kb on + strand at 640.605 kb on + strand at 640.605 kb on + strand at 640.635 kb on + strand, within GFF1310 at 640.635 kb on + strand, within GFF1310 at 640.635 kb on + strand, within GFF1310 at 640.635 kb on + strand, within GFF1310 at 640.635 kb on + strand, within GFF1310 at 640.635 kb on + strand, within GFF1310 at 640.636 kb on - strand, within GFF1310 at 640.636 kb on - strand, within GFF1310 at 640.636 kb on - strand, within GFF1310 at 640.636 kb on - strand, within GFF1310 at 640.636 kb on - strand, within GFF1310 at 640.636 kb on - strand, within GFF1310 at 640.728 kb on + strand, within GFF1310 at 640.728 kb on + strand, within GFF1310 at 640.728 kb on + strand, within GFF1310 at 640.729 kb on - strand, within GFF1310 at 640.729 kb on - strand, within GFF1310 at 640.729 kb on - strand, within GFF1310 at 640.729 kb on - strand, within GFF1310 at 640.729 kb on - strand, within GFF1310 at 640.785 kb on + strand, within GFF1310 at 640.785 kb on + strand, within GFF1310 at 640.785 kb on + strand, within GFF1310 at 640.785 kb on + strand, within GFF1310 at 640.786 kb on - strand, within GFF1310 at 640.786 kb on - strand, within GFF1310 at 640.786 kb on - strand, within GFF1310 at 640.786 kb on - strand, within GFF1310 at 640.871 kb on + strand, within GFF1310 at 640.871 kb on + strand, within GFF1310 at 640.871 kb on + strand, within GFF1310 at 640.872 kb on - strand, within GFF1310 at 640.872 kb on - strand, within GFF1310 at 640.872 kb on - strand, within GFF1310 at 640.872 kb on - strand, within GFF1310 at 640.872 kb on - strand, within GFF1310 at 640.872 kb on - strand, within GFF1310 at 641.020 kb on - strand, within GFF1310 at 641.040 kb on + strand, within GFF1310 at 641.041 kb on - strand, within GFF1310 at 641.041 kb on - strand, within GFF1310 at 641.049 kb on + strand, within GFF1310 at 641.050 kb on - strand, within GFF1310 at 641.050 kb on - strand, within GFF1310 at 641.050 kb on - strand, within GFF1310 at 641.261 kb on + strand at 641.261 kb on + strand at 641.261 kb on + strand at 641.261 kb on + strand at 641.261 kb on + strand at 641.262 kb on - strand at 641.262 kb on - strand at 641.262 kb on - strand at 641.262 kb on - strand at 641.262 kb on - strand at 641.262 kb on - strand at 641.262 kb on - strand at 641.262 kb on - strand at 641.262 kb on - strand at 641.300 kb on + strand at 641.300 kb on + strand at 641.300 kb on + strand at 641.300 kb on + strand at 641.301 kb on - strand at 641.301 kb on - strand at 641.301 kb on - strand at 641.301 kb on - strand at 641.301 kb on - strand at 641.301 kb on - strand at 641.327 kb on + strand at 641.327 kb on + strand at 641.327 kb on + strand at 641.327 kb on + strand at 641.328 kb on - strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.382 kb on - strand at 641.382 kb on - strand at 641.382 kb on - strand at 641.382 kb on - strand at 641.382 kb on - strand at 641.382 kb on - strand at 641.382 kb on - strand at 641.382 kb on - strand at 641.382 kb on - strand at 641.398 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus and calcium pantothenate 10 mg/mL remove 638,054 + GFF1307 0.69 -0.7 638,054 + GFF1307 0.69 +0.5 638,054 + GFF1307 0.69 -0.6 638,054 + GFF1307 0.69 -0.2 638,054 + GFF1307 0.69 -0.2 638,054 + GFF1307 0.69 -0.6 638,054 + GFF1307 0.69 +0.1 638,054 + GFF1307 0.69 +0.8 638,054 + GFF1307 0.69 +0.1 638,055 - GFF1307 0.69 +0.4 638,055 - GFF1307 0.69 -0.7 638,055 - GFF1307 0.69 -0.4 638,055 - GFF1307 0.69 -0.6 638,055 - GFF1307 0.69 +0.6 638,055 - GFF1307 0.69 -0.9 638,055 - GFF1307 0.69 -1.6 638,055 - GFF1307 0.69 -0.3 638,055 - GFF1307 0.69 -0.4 638,057 + GFF1307 0.69 -0.3 638,057 + GFF1307 0.69 +0.0 638,057 + GFF1307 0.69 +2.8 638,058 - GFF1307 0.69 -0.4 638,058 - GFF1307 0.69 -1.6 638,231 + GFF1307 0.82 -0.9 638,231 + GFF1307 0.82 -2.3 638,231 + GFF1307 0.82 +0.2 638,232 - GFF1307 0.82 -0.0 638,232 - GFF1307 0.82 -1.2 638,514 + -1.7 638,514 + -0.1 638,515 - +0.8 638,974 + -0.0 638,974 + -0.6 638,975 - +0.4 638,983 + +0.1 638,983 + -2.3 638,983 + -0.2 638,983 + -0.9 638,983 + +0.8 638,983 + -1.0 638,983 + -0.5 638,984 - +0.1 638,984 - -0.6 638,984 - +1.4 638,984 - -1.9 638,984 - -0.9 638,984 - -1.0 638,984 - +0.2 638,984 - +1.7 638,984 - -0.4 638,984 - -1.4 638,984 - +0.2 639,230 - GFF1309 0.21 -1.2 639,427 + GFF1309 0.33 -0.8 639,427 + GFF1309 0.33 -0.3 639,428 - GFF1309 0.33 +0.4 639,428 - GFF1309 0.33 -0.2 639,428 - GFF1309 0.33 -0.2 639,428 - GFF1309 0.33 -0.4 639,428 - GFF1309 0.33 -0.2 639,428 - GFF1309 0.33 -0.2 639,428 - GFF1309 0.33 -0.5 639,428 - GFF1309 0.33 -1.1 639,428 - GFF1309 0.33 +0.3 639,428 - GFF1309 0.33 +0.3 639,529 + GFF1309 0.39 +0.3 639,529 + GFF1309 0.39 +1.7 639,530 - GFF1309 0.39 -0.3 639,535 + GFF1309 0.39 -0.0 639,535 + GFF1309 0.39 -0.9 639,535 + GFF1309 0.39 +0.3 639,535 + GFF1309 0.39 +0.1 639,536 - GFF1309 0.39 -0.3 639,536 - GFF1309 0.39 +0.1 639,556 + GFF1309 0.40 +0.6 639,557 - GFF1309 0.41 +1.7 639,781 + GFF1309 0.54 -0.4 639,782 - GFF1309 0.54 -0.1 639,782 - GFF1309 0.54 +1.1 639,782 - GFF1309 0.54 -0.1 639,806 - GFF1309 0.56 -0.8 639,806 - GFF1309 0.56 -0.5 639,901 + GFF1309 0.61 -1.6 639,901 + GFF1309 0.61 +0.1 639,901 + GFF1309 0.61 -0.3 639,901 + GFF1309 0.61 -1.9 639,902 - GFF1309 0.61 +0.7 639,902 - GFF1309 0.61 -2.2 639,902 - GFF1309 0.61 -0.6 639,902 - GFF1309 0.61 -0.6 639,902 - GFF1309 0.61 -0.6 639,902 - GFF1309 0.61 -0.1 639,902 - GFF1309 0.61 +1.0 639,902 - GFF1309 0.61 -0.7 639,902 - GFF1309 0.61 +0.4 639,913 + GFF1309 0.62 +0.1 639,913 + GFF1309 0.62 +0.8 639,914 - GFF1309 0.62 +0.7 640,063 + GFF1309 0.71 -0.1 640,063 + GFF1309 0.71 -0.4 640,063 + GFF1309 0.71 -0.9 640,063 + GFF1309 0.71 +0.2 640,063 + GFF1309 0.71 +0.0 640,064 - GFF1309 0.71 +0.2 640,064 - GFF1309 0.71 -0.2 640,064 - GFF1309 0.71 -1.2 640,064 - GFF1309 0.71 -2.5 640,064 - GFF1309 0.71 +0.1 640,064 - GFF1309 0.71 +0.2 640,148 - GFF1309 0.76 +0.6 640,186 + GFF1309 0.79 -0.4 640,186 + GFF1309 0.79 -0.5 640,187 - GFF1309 0.79 -0.8 640,187 - GFF1309 0.79 +0.1 640,187 - GFF1309 0.79 -0.6 640,456 + -0.6 640,456 + +0.9 640,456 + -0.2 640,456 + +0.1 640,456 + +0.7 640,456 + +0.8 640,457 - -0.4 640,457 - -1.1 640,457 - -0.2 640,457 - -0.3 640,457 - -0.9 640,459 + -0.5 640,459 + -0.9 640,460 - -0.1 640,605 + +0.1 640,605 + -0.7 640,605 + -2.7 640,635 + GFF1310 0.14 -0.6 640,635 + GFF1310 0.14 -1.3 640,635 + GFF1310 0.14 -0.5 640,635 + GFF1310 0.14 -0.3 640,635 + GFF1310 0.14 +1.0 640,635 + GFF1310 0.14 -0.2 640,636 - GFF1310 0.14 -0.7 640,636 - GFF1310 0.14 -1.2 640,636 - GFF1310 0.14 -1.4 640,636 - GFF1310 0.14 +0.1 640,636 - GFF1310 0.14 +1.2 640,636 - GFF1310 0.14 -1.8 640,728 + GFF1310 0.28 -0.1 640,728 + GFF1310 0.28 +0.1 640,728 + GFF1310 0.28 -0.1 640,729 - GFF1310 0.28 +0.3 640,729 - GFF1310 0.28 +1.4 640,729 - GFF1310 0.28 -0.7 640,729 - GFF1310 0.28 +1.1 640,729 - GFF1310 0.28 +0.2 640,785 + GFF1310 0.36 -0.2 640,785 + GFF1310 0.36 +0.5 640,785 + GFF1310 0.36 +0.4 640,785 + GFF1310 0.36 -0.6 640,786 - GFF1310 0.37 +0.0 640,786 - GFF1310 0.37 -0.0 640,786 - GFF1310 0.37 -0.1 640,786 - GFF1310 0.37 +1.4 640,871 + GFF1310 0.49 +2.3 640,871 + GFF1310 0.49 +0.2 640,871 + GFF1310 0.49 -0.6 640,872 - GFF1310 0.49 +1.0 640,872 - GFF1310 0.49 -0.2 640,872 - GFF1310 0.49 -0.6 640,872 - GFF1310 0.49 +0.2 640,872 - GFF1310 0.49 +0.4 640,872 - GFF1310 0.49 +0.5 641,020 - GFF1310 0.71 -1.5 641,040 + GFF1310 0.74 +0.0 641,041 - GFF1310 0.74 -1.2 641,041 - GFF1310 0.74 +0.9 641,049 + GFF1310 0.75 -1.6 641,050 - GFF1310 0.76 +0.7 641,050 - GFF1310 0.76 -0.4 641,050 - GFF1310 0.76 -0.3 641,261 + +0.1 641,261 + -0.4 641,261 + -1.4 641,261 + +1.7 641,261 + -0.1 641,262 - +1.1 641,262 - +1.4 641,262 - -2.7 641,262 - -1.2 641,262 - -0.6 641,262 - -0.1 641,262 - +0.0 641,262 - +0.2 641,262 - -0.9 641,300 + -0.7 641,300 + -0.5 641,300 + +0.1 641,300 + -0.7 641,301 - -0.2 641,301 - +0.4 641,301 - -0.2 641,301 - +0.4 641,301 - +0.2 641,301 - +0.2 641,327 + +0.1 641,327 + +1.0 641,327 + +1.2 641,327 + -0.6 641,328 - -0.6 641,381 + +0.5 641,381 + +0.6 641,381 + -0.3 641,381 + +0.4 641,381 + -0.2 641,381 + -0.4 641,381 + +0.4 641,381 + -0.1 641,381 + +0.3 641,381 + +0.3 641,381 + -0.0 641,381 + -1.8 641,381 + +0.5 641,381 + +0.4 641,382 - +0.4 641,382 - -1.3 641,382 - -1.4 641,382 - +0.9 641,382 - -0.0 641,382 - -0.7 641,382 - +0.0 641,382 - -0.5 641,382 - -0.6 641,398 - -0.3
Or see this region's nucleotide sequence